Prednisolone induces osteoporosis-like phenotype in regenerating zebrafish scales

Vrieze, Erik de; Kessel, Maartje A. H. J. van; Peters, Hans; Spanings, F.A.T.; Flik, G.; Metz, Juriaan R.

doi:10.1007/s00198-013-2441-3

Cited by 70 publications

(76 citation statements)

References 47 publications

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“…Beyond advancing our understanding of normal development, postembryonic zebrafish provide valuable models of human diseases and pathologies, including gastrointestinal (Marjoram & Bagnat, 2015; Yang, Tomkovich, & Jobin, 2014) and metabolic disorders (Asaoka, Terai, Sakaida, & Nishina, 2013; Schlegel & Gut, 2015; Seth, Stemple, & Barroso, 2013); neurodegenerative (Babin, Goizet, & Raldúa, 2014; Lee & Freeman, 2014; Schmid & Haass, 2013), behavioral, and neurological pathologies (Kalueff, Stewart, & Gerlai, 2014; Nguyen et al, 2013; Stewart, Braubach, Spitsbergen, Gerlai, & Kalueff, 2014); cancers (White, Rose, & Zon, 2013; Zhao, Huang, & Ye, 2015); osteoporosis (De Vrieze et al, 2014; Laizé, Gavaia, & Cancela, 2014); and numerous other diseases (Ablain & Zon, 2013; Löhr & Hammerschmidt, 2011; Phillips & Westerfield, 2014; Santoriello & Zon, 2012; Shin & Fishman, 2002). Larval and adult zebrafish are further used to model wound healing (Godwin, 2014; Richardson et al, 2013), regeneration (Coffin, Brignull, Raible, & Rubel, 2014; Gemberling, Bailey, Hyde, & Poss, 2013; Wehner & Weidinger, 2015), and behavior (Fero, Yokogawa, & Burgess, 2011; Norton & Bally-Cuif, 2010; Norton et al, 2011), and are frequently used in pharmacological screening and drug discovery (Ali, Champagne, Spaink, & Richardson, 2011; de Esch, Slieker, Wolterbeek, Woutersen, & de Groot, 2012; Jung et al, 2012; Rennekamp & Peterson, 2015).…”

Section: Introductionmentioning

confidence: 99%

Working with zebrafish at postembryonic stages

McMenamin

Chandless

Parichy

2016

Methods in Cell Biology

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As the processes of embryogenesis become increasingly well understood, there is growing interest in the development that occurs at later, postembryonic stages. Postembryonic development holds tremendous potential for discoveries of both fundamental and translational importance. Zebrafish, which are small, rapidly and externally developing, and which boast a wealth of genetic resources, are an outstanding model of vertebrate post-embryonic development. Nonetheless, there are specific challenges posed by working with zebrafish at these stages, and this chapter is meant to serve as a primer for those working with larval and juvenile zebrafish. Since accurate staging is critical for high-quality results and experimental reproducibility, we outline best practices for reporting postembryonic developmental progress. Emphasizing the importance of accurate staging, we present new data showing that rates of growth and size–stage relationships can differ even between wild-type strains. Finally, since rapid and uniform development is particularly critical when working at postembryonic stages, we briefly describe methods that we use to achieve high rates of growth and developmental uniformity through postembryonic stages in both wild-type and growth-compromised zebrafish.

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Section: Introductionmentioning

confidence: 99%

Working with zebrafish at postembryonic stages

McMenamin

Chandless

Parichy

2016

Methods in Cell Biology

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“…Further located on the episquamal side are mono-and multinucleated osteoclasts (De Vrieze et al, 2011). Scale-associated osteoclasts express and show cellular activity of proteolytic enzymes typically involved in resorption: tartrate-resistant acid phosphatase (TRAcP), cathepsin K (CTSK) and matrix metalloproteinase (MMP)-2 and -9 (De Vrieze et al, 2011, 2014Metz et al, 2012). Expression and activity of MMP-2 and MMP-9 have been shown to increase during early scale regeneration, indicative of a role in remodelling of the scale plate (De Vrieze et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Zebrafish (Danio rerio) in calcium-poor water mobilise calcium and phosphorus from scales

et al. 2014

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SummaryCalcium is of vital importance in vertebrates and plasma levels are tightly regulated. Terrestrial vertebrates depend for their calcium uptake exclusively on the diet, while fish have an essentially infinite directly accessible source of calcium in the surrounding water. The need to store calcium seems thus less evident in fish. It was the goal of the present study to investigate the possible role of the scale compartment of zebrafish (Danio rerio) as an accessible pool for calcium and phosphorus. We restricted the calcium availability to zebrafish both in water and diet. Following 12 days restriction, we found that scalar contents of calcium and phosphorus had declined, while magnesium was unaffected, indicating resorption of calcium hydroxyapatite. Osteoclast activity on scales of calcium-restricted fish increased, as we conclude from enlarged demineralized areas on scales, increased TRAcP activity staining, and higher ctsk gene expression. Osteoblasts respond to calcium limitation by increasing expression of col1a2 and alpl. In the presence of water and dietary calcium, removal of scales from one flank of the fish does not affect scalar mineral contents, scale demineralization and osteoclastic TRAcP activity; expression of sp7, alpl and ctsk are up-regulated. Apparently, a high demand for calcium in zebrafish is preferentially complemented with exogenous calcium, while calcium is recruited from the scales when environmental calcium is limited. We conclude that the scales represent an important deposit of available calcium and phosphorus from which these minerals can be recruited in periods of high demand. This finding contributes to a niche for scales as model in bone research.

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“…Besides the amputation model, a fin bone crush model exists [176]. An osteoporosis-like situation can be induced by exposure to prednisolone [177].…”

Section: Zebrafishmentioning

confidence: 99%