Prednisolone and vitamin D(3) modulate oxidative metabolism and cell death pathways in blood and bone marrow mononuclear cells

Shymanskyy, I.; Lisakovska, Olha; Mazanova, Anna; Labudzynskyi, D. О.; Khomenko, Anna; Veliky, M. M.

doi:10.15407/ubj88.05.038

Cited by 8 publications

(7 citation statements)

References 19 publications

(32 reference statements)

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“…VD supplementation significantly decreased MDA levels in women with polycystic ovary syndrome [33]. In addition, VD has been described as responsible for protection against cell damage associated with the CG by regulating cell death pathways and regulating the ROS and NOS synthesis [34]. This information corroborates with our findings.…”

Section: Discussionsupporting

confidence: 92%

The relationship between vitamin D deficiency and oxidative stress can be independent of age and gender

Câmara

Brandão

2021

International Journal for Vitamin and Nutrition Research

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Abstract. The active vitamin D (1,25(OH)2D) acts through a nuclear receptor to perform several functions in cellular metabolism. 1,25(OH)2D participates directly in calcium homeostasis, regulates the immune system, nervous system, blood pressure, insulin secretion, among others. Vitamin D deficiency could also be associated with several diseases and increased cellular oxidative damage. The present study aimed to investigate whether lipid peroxidation and/or protein oxidation are affected by vitamin D deficiency and whether sunlight exposure/diet, gender, and age might influence this relationship. Vitamin D concentrations were obtained from the Heart Hospital database and a questionnaire was applied among the 212 participants. We used the inactive vitamin D (25(OH)2) in the analyses since 1,25(OH)2D has a short half-life and a low blood concentration. Lipid peroxidation and protein oxidation analyses were performed using spectrophotometry. Multivariate analyses suggested the participation of vitamin D deficiency (<30 ng/mL) and sunlight/diet in oxidative stress (p <0.05; R2 MDA: 0.562; R2 CG: 0.429). Multiple linear regression test show that the age and gender of patients are not interfering in the analyses (p>0.05). Therefore, we suggest that the relationship between vitamin D deficiency and oxidative stress can be independent of age and gender.

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Section: Discussionsupporting

confidence: 92%

The relationship between vitamin D deficiency and oxidative stress can be independent of age and gender

Câmara

Brandão

2021

International Journal for Vitamin and Nutrition Research

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“…A close association between reactive oxygen/nitrogen species and apoptosis was suggested, and also reduced complex I activity was demonstrated to have a link to ROS and apoptosis; this may explain the apoptotic effect of prednisolone recorded in this study and supported by others . Meanwhile, MTK attenuated these changes by improvement of mitochondrial dysfunction, oxidative/nitrosative stress, in addition to the antiapoptotic effect of MTK reported by earlier studies …”

Section: Discussionsupporting

confidence: 88%

Montelukast abrogates prednisolone‐induced hepatic injury in rats: Modulation of mitochondrial dysfunction, oxidative/nitrosative stress, and apoptosis

Hegab

El‐Horany

ElBatsh

et al. 2018

J Biochem & Molecular Tox

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The aim of this study was to investigate the protective effect of montelukast (MTK) against prednisolone‐induced hepatic injury in rats. Twenty‐eight male albino rats were categorized into four equal groups. Group I served as the control group; group II: rats orally received prednisolone (5 mg·kg−1·d−1) for 30 days; groups III and IV: rats orally received MTK at 10 and 20 mg·kg−1·d−1, respectively, simultaneously with prednisolone for 30 days. Serum liver enzymes, hepatic mitochondrial function, oxidative/nitrosative stress, and inflammatory and apoptotic markers were evaluated, and the results were confirmed by histopathological examination. MTK showed significant hepatic protection evidenced by alleviated histological lesion and improvement of mitochondrial function, oxidative/nitrosative stress, and inflammatory and apoptotic changes induced by prednisolone, with more profound protection in higher MTK dose (20 mg·kg−1). In view of these findings, we can conclude that MTK may have hepatoprotective potential, beyond its therapeutic value for asthmatic patients during their course of corticosteroid therapy.

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“… 56 This could account for the apoptotic impact of prednisolone observed in this study and others. 57 In a study by Qiu et al , 58 the highest dose of boldine prevented or reduced the apoptotic index of the brain cells, which is associated with a reduction in DNA fragmentation and improved antioxidative status. Several studies have shown that boldine favors anti-inflammatory activities through interfering with the production of free radicals.…”

Section: Discussionmentioning

confidence: 98%

Potential Effects of Boldine on Oxidative Stress, Apoptosis, and Inflammatory Changes Induced by the Methylprednisolone Hepatotoxicity in Male Wistar Rats

et al. 2022

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Background Synthetic glucocorticoid therapeutic agent methylprednisolone (MPL), when used for an extended period of time at high dose, promotes the development of reactive oxygen species (ROS)-induced liver toxicity. This study investigated the role of boldine, a natural antioxidant with anti-apoptotic and anti-inflammatory properties, against MPL-induced hepatoxicity in male Wistar rats. Methods 120 rats were divided into eight equal groups: G1 (control), G2, 3, and 4 (rats orally administered 5, 10, and 50 mg boldine/kg b.w./day; respectively, for 28 days), G5 (rats intramuscularly injected with 100 mg MPL/kg b.w. only on the last three days), G6, 7, and 8 (rats administered boldine + MPL). After the last MPL injection, rats were sacrificed at intervals of 1, 24, and 48 h. Results There was a significant decrease in WBCs, RBCs count, and HGB levels, as well as an increase in PLT count, ALT, AST, TG, and LDL levels, and a decrease in HDL level in serum. Oxidative stress markers levels increased at all times, and gene expression of antioxidant enzymes increased at 24h. Immunohistochemical analysis revealed that cytochrome c levels significantly increased after MPL treatment. The COMET assay revealed detectable DNA lesions. There was no immune reactivity of IL-6 expressions as an inflammatory response marker. Conclusions Oral administration of boldine has a modulatory protective, antioxidant, and anti-apoptotic effect against free radicals.

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Prednisolone and vitamin D(3) modulate oxidative metabolism and cell death pathways in blood and bone marrow mononuclear cells

Abstract: e x p e r i m e n t a l w o r k s e x p e r i m e n t a l w o r k s prednisolone and vitamin d 3 modulate oxidative metabolism and cell death pathways in blood and bone marrow mononuclear cells I. O. ShymaNSkyy, O. O. LISakOVSka, a. O. mazaNOVa, D. O. LaBUDzyNSkyI, a. V. khOmeNkO, m. m

Cited by 8 publications

References 19 publications

The relationship between vitamin D deficiency and oxidative stress can be independent of age and gender

The relationship between vitamin D deficiency and oxidative stress can be independent of age and gender

Montelukast abrogates prednisolone‐induced hepatic injury in rats: Modulation of mitochondrial dysfunction, oxidative/nitrosative stress, and apoptosis

Potential Effects of Boldine on Oxidative Stress, Apoptosis, and Inflammatory Changes Induced by the Methylprednisolone Hepatotoxicity in Male Wistar Rats

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