Predictors of surgical sperm retrieval in non-obstructive azoospermia: summary of current literature

Arshad, Muhammad; Majzoub, Ahmad; Esteves, Sandro C.

doi:10.1007/s11255-020-02529-4

Cited by 47 publications

(42 citation statements)

References 104 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…FSH levels also relate to the proportion of seminiferous tubules exhibiting Sertoli cell-only on testicular biopsies [ 39 ]. Nevertheless, for patients subjected to sperm retrieval, FSH levels do not precisely predict whether spermatogenesis is present [ 40 ]. It is, therefore, possible to find focal sperm-producing areas in the testes of men with NOA-STF and elevated FSH levels during testicular sperm extraction [ 5 , 40 , 41 , 42 , 43 ].…”

Section: Azoospermia Differential Diagnosis: An Overviewmentioning

confidence: 99%

“…Inhibin-B levels reflect Sertoli cell integrity and spermatogenesis status [ 45 ]. However, its diagnostic value seems to be no better than that of FSH, and its use in clinical practice for azoospermia differentiation or sperm retrieval success prediction has not been broadly advocated [ 30 , 40 ].…”

Section: Azoospermia Differential Diagnosis: An Overviewmentioning

confidence: 99%

“…Two karyotypic patterns are typically noticed: non-mosaic (47,XXY; ~85% of cases) and mosaic (47,XXY/46,X; ~15% of cases) [ 12 ]. Residual foci of active spermatogenesis is found on microdissection testicular sperm extraction (micro-TESE) in about 30–50% of KS men [ 12 , 40 , 50 ]. The retrieved sperm may be used for ICSI and generate a healthy child [ 28 , 40 ].…”

Section: Azoospermia Differential Diagnosis: An Overviewmentioning

confidence: 99%

See 2 more Smart Citations

Differential Diagnosis of Azoospermia in Men with Infertility

Andrade

Viana

Esteves

2021

JCM

Self Cite

View full text Add to dashboard Cite

The differential diagnosis between obstructive and nonobstructive azoospermia is the first step in the clinical management of azoospermic patients with infertility. It includes a detailed medical history and physical examination, semen analysis, hormonal assessment, genetic tests, and imaging studies. A testicular biopsy is reserved for the cases of doubt, mainly in patients whose history, physical examination, and endocrine analysis are inconclusive. The latter should be combined with sperm extraction for possible sperm cryopreservation. We present a detailed analysis on how to make the azoospermia differential diagnosis and discuss three clinical cases where the differential diagnosis was challenging. A coordinated effort involving reproductive urologists/andrologists, geneticists, pathologists, and embryologists will offer the best diagnostic path for men with azoospermia.

show abstract

Section: Azoospermia Differential Diagnosis: An Overviewmentioning

confidence: 99%

Section: Azoospermia Differential Diagnosis: An Overviewmentioning

confidence: 99%

Section: Azoospermia Differential Diagnosis: An Overviewmentioning

confidence: 99%

See 1 more Smart Citation

Differential Diagnosis of Azoospermia in Men with Infertility

Andrade

Viana

Esteves

2021

JCM

Self Cite

View full text Add to dashboard Cite

show abstract

“…A 54.5% − 72% sperm retrieval rate was reported in Klinefelter syndrome patients after microTESE [18][19][20] . However, Klinefelter patients with NOA still had a lower sperm retrieval rate than non-Klinefelter patients 14 . In the current study, spermatozoa were harvested from 0% of Klinefelter syndrome patients, which is not comparable with previous research; this is probably due to the low number of Klinefelter syndrome patients included.…”

Section: Discussionmentioning

confidence: 82%

“…One study reported no predictors of successful outcomes, whereas tobacco usage was a predictor factor for patients with a negative TESE 2 . Another recent study concluded that there were no de nite predictors for sperm retrieval, except for chromosome disease; however, Sertoli cell-only histopathology was associated with a reduced chance of harvesting spermatozoa 14 . Our univariate analysis revealed that lower levels of FSH, LH and prolactin, and higher testicular volume and better pathology were predictors of successful sperm retrieval.…”

Section: Discussionmentioning

confidence: 99%

Predictive Factors for Sperm Retrieval in Males With Non-obstructive Azoospermia

Hsu¹,

Chen²,

Chiang³

et al. 2021

Preprint

View full text Add to dashboard Cite

The aim of the current study was to investigate the predictive markers for males with non-obstructive azoospermia (NOA) before they received conventional testicular sperm extraction (cTESE) or microdissection testicular sperm extraction (microTESE). Between January 2010 and December 2020, a total of 56 patients who received cTESE or microTESE surgery at the Urology department of the MacKay Memorial Hospital were included. Our univariate analysis revealed that the following parameters was associated with sperm retrieval: Follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin, testicular volume, histopathology of maturation arrest and Sertoli cell-only. The multivariate analysis showed that Sertoli cell-only was significantly less likely to harvest spermatozoa than normal spermatogenesis (OR = 0.03 (0.002-0.42); p = 0.01). A comparison of cTESE and microTESE revealed that the overall successful sperm retrieval rate was not significantly different between the two methods (74.1% vs. 58.6, p = 0.22). This study demonstrated that lower levels of FSH, LH and prolactin, and a higher testicular volume and better histopathology were associated with a higher sperm retrieval rate in the univariate analysis. In the multivariable analysis, only Sertoli cell-only syndrome appeared to have a significantly negative effect on the successful harvesting of sperm when compared with normal spermatogenesis.

show abstract

Microarray and in silico analysis of DNA repair genes between human testis of patients with nonobstructive azoospermia and normal cells

Karoii

Azizi

Skutella

2022

Cell Biochemistry & Function

View full text Add to dashboard Cite

DNA repair processes are critical to maintaining genomic integrity. As a result, dysregulation of repair genes is likely to be linked with health implications, such as an increased prevalence of infertility and an accelerated rate of aging. We evaluated all the DNA repair genes (322 genes) by microarray. This study has provided insight into the connection between DNA repair genes, including RAD23B, OBFC2A, PMS1, UBE2V1, ERCC5, SMUG1, RFC4, PMS2L5, MMS19, SHFM1, INO80, PMS2L1, CHEK2, TRIP13, and POLD4. The microarray analysis of six human cases with different nonobstructive azoospermia revealed that RAD23B, OBFC2A, PMS1, UBE2V1, ERCC5, SMUG1, RFC4, PMS2L5, MMS19, SHFM1, and INO80 were upregulated, and expression of PMS2L1, CHEK2, TRIP13, and POLD4 was downregulated versus the normal case. For this purpose, Enrich Shiny GO, STRING, and Cytoscape online evaluation was applied to predict proteins’ functional and molecular interactions and then performed to recognize the master pathways. Functional enrichment analysis revealed that the biological process (BP) terms “base‐excision repair, AP site formation,” “nucleotide‐excision repair, DNA gap filling,” and “nucleotide‐excision repair, preincision complex assembly” was significantly overexpressed in upregulated differentially expressed genes (DEGs). BP analysis of downregulated DEGs highlighted “histone phosphorylation,” “DNA damage response, detection DNA response,” “mitotic cell cycle checkpoint signaling,“ and “double‐strand break repair.” Overrepresented molecular function (MF) terms in upregulated DEGs included “Oxidized base lesion DNA N‐glycosylase activity,” “uracil DNA N‐glycosylase activity,” “bubble DNA binding” and “DNA clamp loader activity.” Interestingly, MF investigation of downregulated DEGs showed overexpression in “heterotrimeric G‐protein complex,” “5′‐deoxyribose‐5‐phosphate lyase activity,” “minor groove of adenine‐thymine‐rich DNA binding,” and “histone kinase activity.” Our findings suggest that these genes and their interacting hub proteins could help determine the pathophysiology of germ cell abnormalities and infertility.

show abstract

Predictors of surgical sperm retrieval in non-obstructive azoospermia: summary of current literature

Cited by 47 publications

References 104 publications

Differential Diagnosis of Azoospermia in Men with Infertility

Differential Diagnosis of Azoospermia in Men with Infertility

Predictive Factors for Sperm Retrieval in Males With Non-obstructive Azoospermia

Microarray and in silico analysis of DNA repair genes between human testis of patients with nonobstructive azoospermia and normal cells

Contact Info

Product

Resources

About