Predictive value of the number of frozen blastocysts in live birth rates of the transferred fresh embryos

Abstract

Background Blastocyst development by extended culture in vitro allows the embryos to ‘select’ themselves, thus successful growth to the blastocyst stage is a reflection of the developmental competence of cleavage stage embryos in a cohort. The study aims to determine whether the number of frozen blastocysts is associated with live birth rates of the transferred fresh embryos. Method The retrospective study included 8676 cycles of first fresh embryo transfer from January 2016 to June 2019 at a fertility center of a university hospital. The patients with ≥ 10 oocytes retrieved were divided into three groups according to the number of frozen blastocysts: 0 (group 1), 1–2 (group 2), and ≥ 3 (group 3). The primary outcome measure was the live birth. The secondary outcome measures included clinical pregnancy rates and implantation rates. Logistic regression analysis was also performed. Results Live birth rates in patients with ≥ 3 and 1–2 frozen blastocysts were 47.6% and 46.1%, respectively, which were significantly higher than that in patients without blastocyst (36.0%). The clinical pregnancy rate in group 3 was 65.1%, which was also significantly higher than the other two groups (47.0% and 59.2%). The implantation rates were 35.5%, 47.6%, and 56.0% in the three groups, respectively (P < 0.001). Compared with groups of frozen blastocysts, 0 frozen blastocyst yielded a lower rate of live birth (the adjusted odds ratio: 0.526, 95% CI: 0.469, 0.612). Conclusion In patients with optimal ovarian response that retrieved ≥ 10 oocytes, fresh embryos transfer followed by having blastocysts frozen is a strong indicator of pregnancy achievement, but the number of frozen blastocysts (if not = 0) has limited value in predicting live birth rates.

Retrospective analysis of GnRH-a prolonged protocol for in vitro fertilization in 18,272 cycles in China

“…And the result from 2,226 patients of US suggested that the pregnancy rate was higher when the retrieved oocytes number was ≥ 15 [ 22 ]. A recent study of 8676 cycles of first fresh embryo transfer in the Chinese population detected that the optimal ovarian response for retrieved oocytes was ≥ 10 [ 23 ]. Our results suggest that an oocyte yield of 9 ~ 17 is ideal, giving the highest LBR per OPU cycle (54.0%-60.3%), as well as moderating the risk of OHSS.…”

The effect of preparatory therapy with myo-inositol on the outcome of infertility treatment by ART in men with low reproductive function

“…Генетичні порушення в сперматозоїдах, зокрема фрагментація ДНК, можуть бути причиною зниження чоловічої фертильності. Важливим питанням є визначення здатності сперми низької якості до нормального запліднення ооцитів і подальшого розвитку ембріонів з високим імплантаційним потенціалом [4,5]. Один із головних критеріїв нормального розвитку ембріона -формування бластоцисти на п'яту добу культивування.…”