Predictive value of platelet aggregation rate in postpartum deep venous thrombosis and its possible mechanism

Abstract

The present study investigated the predictive value of the platelet aggregation rate in postpartum deep venous thrombosis and its possible mechanism. From January 2014 to January 2016, 23 patients with postpartum deep vein thrombosis of lower extremity treated in the Department of Obstetrics of Beijing Chaoyang Hospital were as assigned as the observation group. At the same time, 25 cases with normal recovery were assigned as the control group. Blood samples were collected from all the subjects. The platelet aggregation rate was measured using a platelet aggregation apparatus. Plasma platelet activating factor (PAF) levels were measured by ELISA. The positive rate of platelet P-selectin (CD62p) and lysosomal membrane glycoprotein (CD63) was measured by flow cytometry. PI3K expression and AKt phosphorylation levels were measured by western blot analysis. The ROC curve was used to evaluate the value of the platelet aggregation rate in predicting postpartum deep vein thrombosis of lower extremity. The correlation between the platelet aggregation rate and PAF and PI3K/AKt expression was also analyzed. The cesarean section rate, platelet 5-min maximum aggregation rate, PAF level and the positive rate of CD62p and CD63 were significantly higher in the control than those in the observation group (P<0.05). Furthermore, the platelet aggregation rate was positively correlated with the expression of PAF, CD62p and CD63 (r=0.389, 0.451, and 0.452; all P<0.05). The platelet 5-min maximum aggregation rate for predicting postpartum deep vein thrombosis of lower extremity was reflected by the area under the ROC curve (AUC=0.797, P=0.000). The PI3Kp110β/β-actin and p-AKt/AKt ratio was significantly higher in the observation compared with the control group (P<0.05). In addition, the platelet aggregation rate was positively correlated with the expression of PI3K and phosphorylation level of AKt (r=0.441, 0.430; all P<0.05). The results suggested that platelet aggregation activity is elevated in postpartum deep vein thrombosis patients. It has a certain predictive value for the occurrence of postpartum deep vein thrombosis of lower extremity. Thuss, the PI3K/AKt signaling pathway may be one of the mechanisms of platelet aggregation.

Postpartum extracorporeal membrane oxygenation of a woman with COVID-19-related acute respiratory distress syndrome

“…Due to the physiological hypercoagulability postpartum, [ 6 , 7 ] in order to achieve the target ACT value, we used a relatively large initial dose of heparin (approximately 12 U/kg). The ACT increased to 170 s, resulting in bleeding at multiple puncture points, which made it difficult to achieve hemostasis during the surgery.…”

Effect of the expression of CD62P and thrombin generation on patients using central venous catheters for hemodialysis

“…Due to the importance of GPIIb/IIIa platelet receptors in hemostasis, it is critical to investigate the effects of exposure of this glycoprotein while a CVC remains in the vein. P‐selectin is not expressed by platelets at rest in the circulation but is abundantly expressed by activated platelets located in the thrombus, as reported in several studies focusing on platelet activation . Consequently, the investigation of platelet activation and its reflexes in the activation of hemostasis as a whole deserve further study.…”

“…P-selectin is not expressed by platelets at rest in the circulation but is abundantly expressed by activated platelets located in the thrombus, as reported in several studies focusing on platelet activation. [9][10][11][12] Consequently, the investigation of platelet activation and its reflexes in the activation of hemostasis as a whole deserve further study.…”

The Role of Platelet-Selectin as a Marker of Thrombocyte Aggregation on Cerebral Sinus Venous Thrombosis

“…A thrombus or blood clot can form in a vein, artery, heart, or microcirculation and cause complications by causing obstruction or embolism. 10 , 11 A thrombus is an abnormal clot in a vessel with or without leakage. Thrombus is divided into three types, red thrombus (coagulation thrombus), white thrombus (agglutination thrombus), and mixed thrombus.…”

“…In the final stage there is a process of formation of a platelet plug that involves fibrinogen and von Willebrand factor called platelet aggregation. 9 , 10 Light transmission aggregometry is a method used to examine platelet aggregation. This method is the gold standard for examining platelet function.…”