Predictive Value of Plasma MicroRNA-216a/b in the Diagnosis of Esophageal Squamous Cell Carcinoma

Dong, Shuling; Yin, Hang; Dong, Cuicui; Sun, Kang; Lv, Pin; Meng, Weiwei; Ming, Liang; He, Fucheng

doi:10.1155/2016/1857067

Cited by 20 publications

(19 citation statements)

References 36 publications

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“…Accumulative evidences suggested the close involvement of miR-216b in diverse human malignancies. For instance, Dong et al [2016] proposed the predictive value of plasma miR-216a/b in the diagnosis of oesophageal squamous cell carcinoma. Dluzen et al (2016) demonstrated the important role of miR-216b-5p in liver cancer cell lines via regulation of UGT2B expression and activity.…”

Section: Research Articlementioning

confidence: 99%

Long non‐coding RNA Linc00518 promotes paclitaxel resistance of the human prostate cancer by sequestering miR‐216b‐5p

Sun

Geng

et al. 2018

Biology of the Cell

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Background Information To characterise Linc00518 expression in prostate cancer and elucidate the potential mechanistic involvement in paclitaxel resistance, the relative expression of Linc00518 and miR‐216b‐5p was determined by real‐time PCR. The regulatory effect of miR‐216b‐5p on either Linc00518 or GATA6 was interrogated with luciferase reporter assay. The endogenous GATA6 protein was analysed by Western blotting. The cell viability was measured by MTT assay and IC50 of paclitaxel was calculated through cell counting. Results Linc00518 was highly expressed in prostate tumour both in vivo and in vitro. High level of Linc00518 transcripts associated with paclitaxel resistance. Linc00518 competitively inhibited miR‐216b‐5p through sponging mechanism. Linc00518 deficiency compromised the paclitaxel resistance in the acquired resistance cell lines. Conclusions and Significance We demonstrated that overexpression of Linc00518 contributed to the paclitaxel resistance in prostate cancer via sequestering miR‐216b‐5p.

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Section: Research Articlementioning

confidence: 99%

Long non‐coding RNA Linc00518 promotes paclitaxel resistance of the human prostate cancer by sequestering miR‐216b‐5p

Sun

Geng

et al. 2018

Biology of the Cell

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“…In particular, published studies have demonstrated that dysregulated miRNA patterns (upregulation and/or downregulation) contribute to tumor initiation and progression in various malignancies via the modulation of target genes involved in proliferation, apoptosis, angiogenesis, invasion, and migration 5,6. In addition, from a clinical practice perspective, the value of miRNAs as diagnostic and prognostic markers has been revealed in recent years 7,8…”

Section: Introductionmentioning

confidence: 99%

The prognostic value of decreased miR-101 in various cancers: a meta-analysis of 12 studies

Hu¹,

Wu²,

Zhao³

et al. 2017

OTT

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BackgroundA consensus regarding the prognostic value of decreased miR-101 in human cancers has not been reached. This study aimed to comprehensively investigate the internal associations between loss of miR-101 expression and prognostic implications in patients with cancer.Materials and methodsAll relevant literature in electronic databases, including PubMed, ISI Web of Science, and Embase, up to March 1, 2017 were searched. Correlations between decreased miR-101 and clinicopathological parameters were defined by odds ratios (ORs). The degree of association between reduced miR-101 and survival outcome was evaluated by pooled hazard ratios (HRs) and relevant 95% CIs.ResultsTwelve eligible studies with 2,088 patients were included in this meta-analysis. Decreased miR-101 expression was closely connected with poor overall survival, with a pooled HR of 2.15 (95% CI 1.71–2.7, P<0.001). This correlation was also revealed when stratified analysis was conducted with respect to ethnicity, cancer type, sample size, specimen source, and analysis model. However, decreased miR-101 was not associated with disease-free survival, recurrence-free survival, or progression-free survival, with a pooled HR of 1.59 (95% CI 0.83–3.03, P=0.128), despite a positive trend. In addition, reduced miR-101 was intimately related to poorer tumor differentiation (OR 2.17, 95% CI 1.14–4.13; P=0.019), advanced tumor classification (OR 5.25, 95% CI 3.39–8.12; P<0.001), and higher TNM stage (OR 6.18, 95% CI 3.79–10.09; P<0.001).ConclusionOur findings suggest that loss of miR-101 expression is correlated with worse overall survival in a variety of cancers, and could serve as a predictive indicator for clinicopathological features. Furthermore, miR-101 may become a feasible therapeutic target in most human cancers.

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“…Moreover, our findings showed less expression of hsa-miR-216 in achalasia. Reduced expression of miR-216 has also been shown in other diseases, such as nonsmall cell lung cancer [31], ESCC [32], nasopharyngeal carcinoma [33] and hepatocellular carcinoma [34]. Down-regulation of miR-217 and miR-216 that act as tumor suppressors, may also explain the high risk of esophageal cancer in achalasia patients.…”

Section: Discussionmentioning

confidence: 95%

Alterations in esophageal microRNAs expression in primary esophageal achalasia by next-generation sequencing

Gholipour

Mikaeli

Mowla

et al. 2020

Preprint

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Background The molecular knowledge of primary esophageal achalasia is essential for the early diagnosis and treatment of this neurodegenerative motility disorder. So it is substantial to find the main microRNAs (miRNAs), which are related to mechanisms of achalasia. Methods This study aimed to determine some patterns of deregulated miRNAs in achalasia. This case-control study was performed on 52 achalasia patients and 50 non-achalasia controls. The miRNA expression profiling was conducted on esophageal tissue samples from achalasia and non-achalasia patients, via next-generation sequencing (NGS). Differential expression of miRNAs was analyzed by edgeR software. The selected dysregulated miRNAs were additionally confirmed using RT-qPCR. Potential target genes of the down-regulated and up-regulated miRNAs were also predicted to understand the putative role of the miRNAs in achalasia. Results We identified 15 miRNAs that were significantly altered in the achalasia tissues compared to controls. Among these miRNAs, three; miR-133a-5p, miR-143-3p and miR-6507-5p were up-regulated. Also we found six miRNAs; miR-215-5p, miR-216a-5p, miR-216b-5p, miR-217, miR-7641 and miR-194-5p were down-regulated significantly. The predicted targets for the dysregulated miRNAs showed significant disease-associated pathways like neuron cell apoptosis, neuromuscular balance, neuron growth factor signaling and immune response regulation. Gene expression analysis confirmed significant downregulation of hsa-miR-217 (p-value =0.004) in LES of achalasia patients with significant enrichment in myelination process ontology. This study provides the first integrated miRNA expression profile using NGS in achalasia. Our findings introduced 15 candidate microRNAs as achalasia associated non-coding RNAs genes showing confirmed downregulation of the hsa-miR-217 in Achalasia disease. Conclusions Our results may serve as a basis for more future functional studies to discover the role of candidate miRNAs in the etiology of achalasia and their application in diagnosis and probably treatment.

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Predictive Value of Plasma MicroRNA-216a/b in the Diagnosis of Esophageal Squamous Cell Carcinoma

Cited by 20 publications

References 36 publications

Long non‐coding RNA Linc00518 promotes paclitaxel resistance of the human prostate cancer by sequestering miR‐216b‐5p

Long non‐coding RNA Linc00518 promotes paclitaxel resistance of the human prostate cancer by sequestering miR‐216b‐5p

The prognostic value of decreased miR-101 in various cancers: a meta-analysis of 12 studies

Alterations in esophageal microRNAs expression in primary esophageal achalasia by next-generation sequencing

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