Predictive value of human papillomavirus type for histological diagnosis of women with cervical cytological abnormalities

Burger, M. P. M.; Hollema, Harry; Pieters, W. J. L. M.; Quint, Wim

doi:10.1136/bmj.310.6972.94

Cited by 23 publications

(15 citation statements)

References 6 publications

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“…An improvement in the ability to predict highgrade lesions could also have important clinical implications. 6 In this study HPV testing afforded an additional 7% to 20% benefit, increasing the probability of highgrade lesions from 29% to 36% to 49%.…”

Section: Commentmentioning

confidence: 79%

Human papillomavirus deoxyribonucleic acid detection in mildly or moderately dysplastic smears: A possible method for selecting patients for colposcopy

Bollen

Tjong-A-Hung

Velden

et al. 1997

American Journal of Obstetrics and Gynecology

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Human papillomavirus deoxyribonucleic acid detection in mildly or moderately dysplastic smears: a possible method for selecting patients for colposcopy Bollen, L.J.M.; Tjong-a-Hung, S.P.; van der Velden, J.; Brouwer, K.; Mol, B.W.J.; ten Kate, F.J.W.; ter Schegget, J. Published in:American journal of obstetrics and gynecology DOI:10.1016/S0002-9378(97)70144-3 Link to publicationCitation for published version (APA): Bollen, L. J. M., Tjong-a-Hung, S. P., van der Velden, J., Brouwer, K., Mol, B. W. J., ten Kate, F. J. W., & ter Schegget, J. (1997). Human papillomavirus deoxyribonucleic acid detection in mildly or moderately dysplastic smears: a possible method for selecting patients for colposcopy. American journal of obstetrics and gynecology, 177, 548-553. https://doi.org/10.1016/S0002-9378(97)70144-3 General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Amsterdam and Zaandam, The Netherlands OBJECTIVE: Current screening protocols for cervical cancer dictate that patients with smears read as mild or moderate dysplasia of the uterine cervix undergo colposcopy, although approximately half these women do not prove to have high-grade squamous intraepithelial lesions. The aim of this study was to determine whether human papillomavirus testing is capable of discriminating between high-and Iowgrade squamous intraepithelial lesions so as to be useful in reducing the number of colposcopic examinations. STUDY DESIGN: We tested 190 consecutive patients with smears read as mild or moderate dysplasia for the presence of human papillomavirus deoxyribonucleic acid by use of two different polymerase chain reactions with the consensus primer pairs CPI/IIG and MY09/11. Typing was carried out by direct sequence analysis of the CPI/IIG amplimers. The MY09/11 amplimers were detected in enzyme-linked immunosorbent assay format with the SHARP (Solution Hybridization Assay for PCR Products) Signal System with two probe mixtures (A and B) to detect nononcogenic and oncogenic human papillomavirus types. The human papillomavirus test results were compared with the histologic diagnosis, which was regarded as the reference standard. RESULTS" Fifty-six of the 190 patients had high-grade squamous intraepithelial lesions. The sensitivity was 96% for the CPI/IIG test and 95% for the MY09/11 polymerase...

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Section: Commentmentioning

confidence: 79%

Human papillomavirus deoxyribonucleic acid detection in mildly or moderately dysplastic smears: A possible method for selecting patients for colposcopy

Bollen

Tjong-A-Hung

Velden

et al. 1997

American Journal of Obstetrics and Gynecology

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“…Therefore, 265 newly diagnosed patients with CIN were included in the study. Some of these patients were also included in previous studies on HPV and CIN (Burger et al, , 1995 6, 11, 16, 18, 31 and 33 separately (Melchers et al, 1989;Claas et al, 1989). If the general primer-mediated reaction was positive but the reactions for types 6,11,16,18,31 and 33 were negative, the type remained unknown.…”

Section: Materials and Methods Patientsmentioning

confidence: 99%

Epidemiological evidence of cervical intraepithelial neoplasia without the presence of human papillomavirus

Burger¹,

Hollema²,

Pieters³

et al. 1996

Br J Cancer

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Summary The aim of this paper was to provide epidemiological evidence to support the notion that cervical intraepithelial neoplasia (CIN) without human papillomavirus (HPV) is a true entity. If a diagnosis of HPVnegative cervical neoplasia is erroneous, one would not expect there to be any differences in risk factors between HPV-positive and HPV-negative patients. Patients at a gynaecological outpatient clinic of a university hospital [a total of 265 consecutive women with dyskaryotic cervical smears who were subsequently diagnosed with CIN I (n = 37), CIN II (n = 48) or CIN III (n = 180)] completed a structured questionnaire regarding smoking habits and sexual history. Analysis of an endocervical swab for Chlamydia trachomatis, analysis of a cervical scrape for HPV, and morphological examination of cervical biopsy specimens were also performed. HPV was found in 205 (77.4%) out of the 265 women. Univariate analysis showed that current age (P= 0.02), current smoking behaviour (P = 0.002) and the number of sexual partners (P = 0.02) were significantly associated with the presence of HPV. Age at first sexual intercourse, a past history of venereal disease or genital warts, and current infection with Chiamydia trachomatis were not associated with the presence of HPV. Using multivariate logistic regression analysis, the number of sexual partners and current smoking behaviour showed an independent significant association with HPV. HPV-negative and HPV-positive CIN patients differ with respect to the risk factors for HPV. These findings suggest that HPV-negative CIN is a separate true entity.Keywords: cervix dysplasia; papillomavirus; risk factor Cervical intraepithelial neoplasia (CIN) is a morphologically defined lesion associated with the development of cervical carcinoma. In the conventional morphogenetic model CIN is separated into three grades according to the degree of cellular atypia and disturbance of the epithelial architecture (Richart, 1973). The infection with human papillomavirus (HPV) is strongly associated with cervical neoplasia. HPV shows considerable genetic heterogeneity (De Villiers, 1989), and a great diversity of HPV types is found in CIN Lungu et al., 1992; Bergeron et al., 1992). Known risk factors for cervical HPV infection are a comparatively young age and an increased lifetime number of sexual partners (Schiffman, 1994;Woodman, 1994). Smoking has also been identified as a risk factor 95%. Franco (1994) reported that this figure was still being scrutinised by the authors and it was likely that additional HPV-positive samples would be declared, raising the detection rate to very close to 100%. Such a result would be at odds with previous observations that HPV-negative and HPV-positive patients with squamous cell cancer of the cervix differ with respect to age and prognosis (Higgins et al., 1991;Riou et al., 1990). Opinions regarding the prevalence of HPV in CIN are fairly consistent. Schiffman (1994) mentioned that in their studies the prevalence of HPV in definite cases of CIN approached ...

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“…The most important types causing cervical cancers are referred to as high-risk types, which include 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 73, and 82 (3)(4)(5)(6)(7)(8)(9). The types 26, 23, 66 have also been found to be included in high risks types (9,10). Two high-risk types 16 & 18 are the most causative agents of cervical cancer in Iran.…”

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confidence: 99%

“…Two high-risk types 16 & 18 are the most causative agents of cervical cancer in Iran. High-risk HPV diagnosis in women susceptible to progressive uterine lesions is of great importance (10,11). The high prevalent of HPV types causing cervical cancer necessitate, developing a reliable method for detecting different types of HPV for the management and preventing the disease.…”

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confidence: 99%

Qualitative-based Multiplex PCR: Diagnosis of Papilloma virus Types 16 & 18 in Samples Taken from Patients with Malignant Lesions

Shafaati¹,

Akhavan²,

Yazdani³

et al. 2015

Iran J Virol

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Background and Aims: The human papilloma virus was introduced as the major etiological agent in outbreak of cervical cancer in 1970. Since it is very difficult to recognize these viruses and their types using the serological tests and cell culture, molecular methods such as PCR are of great importance. Therefore, in this study, our goal was to use a multiple specific PCR assay on L1 and E6 genes of HPV for molecular detection of this virus and its common type's detection in the society. Materials and Methods: After collecting the samples from malignant lesions of various patients, the viral DNA was extracted from paraffin blocks of 50 clinical samples and the PCR method was performed on the mentioned samples by specific primers for L1 and E6 genes together with β-globin (as internal control). The PCR product was analyzed on 2℅ agarose gel and the sensitivity of this test was examined. Results: from among 50 samples of the patients, 33 cases were HPV positive and 17 ones were negative. The sensitivity of this test was 20 copies from recombinant construct containing target genes for each reaction. Conclusion: this study confirmed that the designed PCR with specific primers on L1 and E6 genes of HPV proved to be an accurate method for detecting and determining the HPV types.

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Predictive value of human papillomavirus type for histological diagnosis of women with cervical cytological abnormalities

Cited by 23 publications

References 6 publications

Human papillomavirus deoxyribonucleic acid detection in mildly or moderately dysplastic smears: A possible method for selecting patients for colposcopy

Human papillomavirus deoxyribonucleic acid detection in mildly or moderately dysplastic smears: A possible method for selecting patients for colposcopy

Epidemiological evidence of cervical intraepithelial neoplasia without the presence of human papillomavirus

Qualitative-based Multiplex PCR: Diagnosis of Papilloma virus Types 16 & 18 in Samples Taken from Patients with Malignant Lesions

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