Predictive value of follicular fluid insulin like growth factor-1 in IVF outcome of normo-ovulatory women

Faraj, Noura; Alhalabi, Marwan; Quobaili, Faizeh Al

doi:10.1016/j.mefs.2017.01.002

Cited by 3 publications

(3 citation statements)

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“…Results of our study in which we analysed pure samples of mural granulosa cells in different-sized follicles of ovaries which were all in the same developmental stage, indicate that large differences between smaller and larger follicles in the healthy antral follicle pool already exist at the start of the follicular phase, as smaller follicles still show high levels of granulosa cell proliferation for follicle growth, while larger follicles are already differentiated into more mature follicles. This is exemplified by the higher ANGPT1 expression, which improves vascularity, and higher IGF1 expression in granulosa cells of larger follicles, which have both been associated with improved human IVF outcomes (Faraj et al, 2017;Vural et al, 2016). This implies that including smaller follicles for oocyte aspiration might have negative consequences for IVF outcome.…”

Section: Discussionmentioning

confidence: 99%

“…IGF1 is considered a follicular maturation marker (May and Schomberg, 1984) and ANGPT1 is an angiogenic factor that is necessary for blood vessel maturation and its expression increases in granulosa cells in late stages of follicular development in preparation of ovulation and luteinization (Robinson et al, 2009;Hayashi et al, 2003;Hazzard et al, 1999). In addition, higher follicular ANGPT1 and IGF1 expression have both been associated with improved human in vitro fertilization (IVF) outcomes (Faraj et al, 2017;Vural et al, 2016). Together this indicates that larger follicles in the pool are more mature and have a higher developmental competence as compared to the smaller follicles in the pool.…”

Section: Characteristics Associated With Follicle Sizementioning

confidence: 99%

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Physiological and molecular aspects of ovarian follicular developmental competence in sows

Costermans¹

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Chapter 1 General IntroductionChapter 2 Follicular development of sows at weaning in relation to estimated breeding value for within-litter variation in piglet birth weight Chapter 3 In ovaries with high or low variation in follicle size, granulosa cells of antral follicles exhibit distinct size-related processes Chapter 4 Follicular fluid steroid profile in sows: relation to follicle size and 77 oocyte quality Chapter 5 Steroid profile of porcine follicular fluid and blood serum: relation with follicular development Chapter 6 Consequences of negative energy balance on follicular development and oocyte quality in primiparous sows Chapter 7 Influence of the metabolic state during lactation on milk production in modern sows Chapter 8 General Discussion Appendices Summary of main findings Acknowledgements About the author Curriculum vitae List of publications 208 Education statement 209 General IntroductionGeneral Introduction 9 Research gapsTo conclude, reproductive problems in sows, such as the previously described trend towards lower piglet birth weights and associated pre-weaning mortality in highly prolific sows, may originate (at least partly) from impairments in the antral follicle pool from which follicles are selected to become ovulatory follicles, potentially giving rise to the next litter. The underlying processes, which determine, and influence follicle developmental competence remain poorly understood. Although previous studies have attempted to identify physiological characteristics of follicular developmental competence, these studies had variable outcomes. The main physiological characteristics that were studied are antral follicle size, where larger antral follicles either might yield better quality oocytes with improved blastocyst formation and implantation rates (

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Section: Discussionmentioning

confidence: 99%

Section: Characteristics Associated With Follicle Sizementioning

confidence: 99%

Physiological and molecular aspects of ovarian follicular developmental competence in sows

Costermans¹

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“…The beneficial effects of IGF-1 exposure during oocyte maturation will also influence subsequent embryo development. Studies on human IVF patients have shown the concentration of IGF-1 in FF at oocyte recovery significantly correlated with embryo quality and clinical pregnancy rate (Mehta et al 2013;Faraj et al 2017). Clearly IGF-1 has an important role in ensuring full developmental competency of oocytes and embryos.…”

Section: Lactationmentioning

confidence: 99%

Factors affecting oocyte quality in New Zealand dairy cows

Reed¹

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Oocyte quality is an important factor affecting the reproductive performance of dairy cows. Good oocyte quality is essential for embryonic survival through early pregnancy as maternal transcripts, cellular machinery, and regulatory factors required by the embryo are derived from the oocyte. Early embryonic development coincides with a high rate of pregnancy loss in New Zealand dairy cows, suggesting that poor oocyte quality is limiting their reproductive efficiency (Berg et al. 2017). The research presented in this thesis investigated the effect of genetic merit for fertility and uterine infections on oocyte quality in New Zealand dairy cows. I also sought to determine the characteristics of a good follicular microenvironment that is capable of supporting the development of a high-quality oocyte, and potentially identify biomarkers associated with fertility. To achieve the objective of this thesis, I assessed oocyte quality and the corresponding follicular microenvironment in dairy cattle with divergent fertility breeding values (FBV) across multiple life stages. To this end, cumulus-oocyte complexes (COC) and follicular fluid (FF) were recovered from heifers, lactating cows, and mature non-lactating cows of High-and Low-FBV. Molecular biomarkers within individual cumulus cell masses and oocytes were used to assess oocyte quality. Follicular concentrations of amino acids, metabolites, and steroids were quantified to elucidate the follicular microenvironment. While oocyte quality and follicular microenvironment appeared to be subtly different between the High-and Low-FBV animals throughout their life, these differences were at their greatest during lactation when oocyte quality is clearly superior in High-FBV cows. This result was not unexpected as FBV is calculated solely from phenotypes measured in lactating cows. Furthermore, previous research has indicated that the advantage of a higher FBV is greatest when the cow’s fertility is challenged, as occurs during lactation (Craig et al. 2018). Another objective of this thesis was to identify biological predictors of fertility, particularly those apparent in early life. Altered adrenal function appeared to be related to the sub-fertility of Low-FBV cows at all life stages investigated. As such, differences in circulating adrenal steroids could potentially provide a marker of poor fertility. I also compared oocyte quality and the follicular microenvironment between lactating cows that did or did not conceive on the subsequent oestrus, regardless of FBV. This facilitated the identification of novel markers of fertility that would not have been captured by the current FBV. The preliminary evidence of biological markers associated with fertility presented in this thesis may direct future research. This thesis also investigated the contribution of oocyte quality to the sub-fertility of cows experiencing an acute uterine infection. Oocyte quality was not different between cows with or without a uterine infection despite changes in the follicular microenvironment. This may have been partially due to the follicular stage sampled, the model of uterine infection, or the small number of animals sampled. It is possible that had the oocyte been allowed to fully mature within the altered follicular microenvironment of the infected cows, differences in oocyte quality would have been identified. Overall, the results presented in this thesis extend our understanding of oocyte quality and the follicular microenvironment of growing dominant and preovulatory follicles in pasture-fed dairy cows.

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In ovaries with high or low variation in follicle size, granulosa cells of antral follicles exhibit distinct size-related processes

Costermans

Keijer

Schothorst

et al. 2019

Molecular Human Reproduction

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Antral follicle size might be a valuable additive predictive marker for IVF outcome. To better understand consequences of antral follicle size as a marker for reproductive outcome, we aimed to obtain insight in follicle size-related granulosa cell processes, as granulosa cells play an essential role in follicular development via the production of growth factors, steroids and metabolic intermediates. Using the pig as a model, we compared gene expression in granulosa cells of smaller and larger follicles in the healthy antral follicle pool of sows, which had a high variation versus low variation in follicle size. Selected gene expression was confirmed at the protein level. Granulosa cells of smaller antral follicles showed increased cell proliferation, which was accompanied by a metabolic shift towards aerobic glycolysis (i.e. the Warburg effect), similar to other highly proliferating cells. High granulosa cell proliferation rates in smaller follicles might be regulated via increased granulosa cell expression of the androgen receptor and the epidermal growth factor receptor, which are activated in response to locally produced mitogens. While granulosa cells of smaller follicles in the pool are more proliferative, granulosa cells of larger follicles express more maturation markers such as insulin-like growth factor-1 (IGF1) and angiopoietin 1 (ANGPT1) and are therefore more differentiated. As both higher IGF1 and ANGPT1 have been associated with better IVF outcomes, the results of our study imply that including smaller follicles for oocyte aspiration might have negative consequences for IVF outcome.

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Predictive value of follicular fluid insulin like growth factor-1 in IVF outcome of normo-ovulatory women

Cited by 3 publications

References 21 publications

Physiological and molecular aspects of ovarian follicular developmental competence in sows

Physiological and molecular aspects of ovarian follicular developmental competence in sows

Factors affecting oocyte quality in New Zealand dairy cows

In ovaries with high or low variation in follicle size, granulosa cells of antral follicles exhibit distinct size-related processes

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