Predictive role of histological features and Ki67 pattern on high‐risk HPV presence in atypical cervical lesions

Evaluation of cervical cone biopsies for coexpression of p16INK4a and Ki‐67 in epithelial cells

“…3,6,10 Ki-67 expression alone has been found in previous studies to be associated with the grade of dysplasia. 28,29 There are few studies that evaluated p16 INK4a and Ki-67 immunohistochemistry in HPV-induced dysplastic lesions and showed correlation with progression grade of the lesion. 30,31 We used a cocktail of both antibodies in one staining procedure simultaneously, thus allowing to evaluate whether p16 INK4a and Ki-67 are coexpressed within exactly the same cell and to link these findings to the grade of histopathologic abnormality of the cervical squamous epithelium.…”

“…1) Ki-67 expression alone has been found in previous studies to be associated with the grade of dysplasia. 28,29 There are few studies that evaluated p16…”

Cervical Neoplasia: Papillomavirus Infection and Resistance to Anti Tumor Immunity

“…different basal cells) can be present in different ways, either localized or diffuse. This difference in proportion raises the question of the differential diagnosis between the changes associated with human papillomavirus infection and those relational or metaplastic [67]. The different proportions observed in the different studies may also be due to the different techniques used to identify these alterations.…”

“…These authors observe that: koilocytosis is absent in 55.5% of CIN1 and 5.5% CIN2 and in no case classified as CIN3 [66]. Analyzing the histological criteria for the diagnosis of human papillomavirus infection, the authors find that koilocytosis is observed in 34% of patients with grade 1 cervical intraepithelial neoplasia [67]. In the prognostic and reproducibility study of koilocytosis in cervical intraepithelial neoplasms, koilocytosis was found in 62% of CIN 1s and 70% of CIN 2s [68].…”

The role of cytopathology in diagnosing HPV induced oropharyngeal lesions

“…High prevalence of koilocytes has been reported in tissue sections of OSCC and it has been found to be equally distributed among the different tumor grades 14. Cabibi et al reported that the sensitivity of detection of HPV infection by identification of koilocytes was 74% and the specificity was 72% 15. Many studies have further confirmed this histologic evidence of HPV by performing molecular biology techniques like in situ hybridization (ISH) and polymerase chain reaction (PCR), to demonstrate HPV DNA in those lesions 16.…”