Predictive power of the Triticum root elongation test for the assessment of novel anti‑proliferative therapies

Olaru, Octavian Tudorel; Zanfirescu, Anca; Nițulescu, George Mihai; Dinu-Pîrvu, Cristina-Elena; Anuța, Valentina; Tsatsakis, Aristidis; Spandidos, Demetrios Α.; Margină, Denisa; Șeremet, Oana Cristina

doi:10.3892/ijmm.2019.4192

Cited by 4 publications

(6 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cytological assessments and root growth inhibition measurements are practical methods for assessing the biological activity of novel antineoplastic agents and acute toxicity in preliminary non-clinical studies (2,17).…”

Section: Discussionmentioning

confidence: 99%

“…The inhibition of the root growth and the observation of the cytological parameters is a simple, and yet efficient method to evaluate new potential anticancer agents and can provide useful information about the genotoxicity risks (1). The Triticum root elongation test can be used to detect new compounds with anti-proliferative effects on cancer cells, particularly those compounds targeting tubulin and may be an effective tool with which to identify novel spindle inhibitors (2). The use of phytobiological tests has major advantages, such as a more rapid outcome, a simplified operative procedure and lower costs (3).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of ultrasound exposure on the Triticum aestivum root elongation test

et al. 2019

Self Cite

View full text Add to dashboard Cite

The Triticum aestivum root elongation test can be used as a simple and effective method for the identification of novel potential anti-proliferative agents. The employment of this method is based on the fact that a significant number of oncogenic signal transduction pathways are highly conserved in living organisms. Moreover, the use of phytobiological assays has several advantages, such as rapid outcomes, simple procedure and reduced costs. Previous studies have demonstrated that ultrasound exposure can have lethal effects on small organisms, medium and high frequency exposure, leading to cell destruction. In the present study, the effects of ultrasound exposure on the radicular length of Triticum caryopses were analyzed as a potential tool for further experimentation using ultrasounds as a stress model in phytobiological tests. The influence of the exposure time was determined at different intervals and was proven to exert significant effects. Short intervals of <5 min produced no statistically significant effects on root elongation, and the maximum of inhibition was achieved following 30 min of exposure. The experimental results highlighted that the inhibitory effect of ultrasound irradiation was lower following 15 min of exposure than after a shorter interval of 10 min, emphasizing a complex, non-linear biological response. On the whole, the findings of this study indicated a complex mechanism for the inhibition of the radicular growth by ultrasound exposure. The ultrasound exposure of Triticum seeds may be used as a tool to either assess the effects of toxic agents following an increase in cell permeability, or to examine the synergistic action between ultrasounds and exposure to chemical mixtures that can promote physiological disruptions.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of ultrasound exposure on the Triticum aestivum root elongation test

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…They may represent an alternative to reduce the number of animals used in anticancer research. Olaru et al (2019) conducted a study to validate the Triticum aestivum root elongation assay as a simple and efficient alternative tool for the assessment of the potential of novel anti-proliferative agents. They tested 20 anti-proliferative compounds in the Triticum aestivum root elongation assay and compared the results with the NCI60 human tumor cell line anti-proliferative profile.…”

Section: Genotoxicity Assessment Using Triticum Aestivum Assaymentioning

confidence: 99%

“…They tested 20 anti-proliferative compounds in the Triticum aestivum root elongation assay and compared the results with the NCI60 human tumor cell line anti-proliferative profile. The results of the study pointed out that the Triticum test used alone has significant limitations, due to false-negative results, but in combination with other bioassays can be a useful tool to detect novel antiproliferative agents, particularly those targeting tubulin (Olaru et al, 2019).…”

Section: Genotoxicity Assessment Using Triticum Aestivum Assaymentioning

confidence: 99%

Triticum aestivum Assay - A Useful Tool for Environmental Monitoring and Toxicity Assessment

Jităreanu

Caba

Trifan

et al. 2019

Not Bot Horti Agrobo

View full text Add to dashboard Cite

The present review summarizes the literature data regarding the application of Triticum aestivum assay as an alternative method for toxicity assessment of environmental pollutants or potential therapeutic agents. Plant bioassays present several advantages among other biological assays (simplicity, low cost, rapid test activation, a wide array of assessment endpoints). They present a good correlation with animal and human cells models, and are a reliable tool for genotoxicity assessment. Furthermore, in the context of toxicology guidelines that promote the substitution of assays using animal models with other bioassays, genotoxicity assays using higher plants models have gained in popularity. The present review focuses on three major aspects regarding Triticum aestivum assay - its utility in environmental pollution monitoring, its application in genotoxicity assessment studies, and its application in phytotoxicity evaluation of nanomaterials. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

show abstract

“…Data analysis tools such as the NCI's Developmental Therapeutics Program (DTP)'s COMPARE algorithm use these outputs on all 60 cancer cell lines to create a fingerprint profile that allows classification and can predict the mechanism of action [29,30]. The fingerprint of cellular response in the NCI-60 assay can be used to determine similar prototype compounds, and the usefulness of this data-mining approach has been demonstrated in various studies [31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

In Search of Outliers. Mining for Protein Kinase Inhibitors Based on Their Anti-Proliferative NCI-60 Cell Lines Profile

Ion

Nițulescu

2020

Molecules

View full text Add to dashboard Cite

Protein kinases play a pivotal role in signal transduction, protein synthesis, cell growth and proliferation. Their deregulation represents the basis of pathogenesis for numerous diseases such as cancer and pathologies with cardiovascular, nervous and inflammatory components. Protein kinases are an important target in the pharmaceutical industry, with 48 protein kinase inhibitors (PKI) already approved on the market as treatments for different afflictions including several types of cancer. The present work focuses on facilitating the identification of new PKIs with antitumoral potential through the use of data-mining and basic statistics. The National Cancer Institute (NCI) granted access to the results of numerous previously tested compounds on 60 tumoral cell lines (NCI-60 panel). Our approach involved analyzing the NCI database to identify compounds that presented similar growth inhibition (GI) profiles to that of existing PKIs, but different from approved oncologic drugs with other mechanisms of action, using descriptive statistics and statistical outliers. Starting from 34,000 compounds present in the database, we filtered 400 which displayed selective inhibition on certain cancer cell lines similar to that of several already-approved PKIs.

show abstract

Predictive power of the Triticum root elongation test for the assessment of novel anti‑proliferative therapies

Cited by 4 publications

References 40 publications

Effect of ultrasound exposure on the Triticum aestivum root elongation test

Effect of ultrasound exposure on the Triticum aestivum root elongation test

Triticum aestivum Assay - A Useful Tool for Environmental Monitoring and Toxicity Assessment

In Search of Outliers. Mining for Protein Kinase Inhibitors Based on Their Anti-Proliferative NCI-60 Cell Lines Profile

Contact Info

Product

Resources

About