Predictive kinetic optimisation of hydrophilic interaction chromatography × reversed phase liquid chromatography separations: Experimental verification and application to phenolic analysis

Recent Developments in Two-Dimensional Liquid Chromatography: Fundamental Improvements for Practical Applications

“…The chromatographic method was optimized using predictive Pareto optimization. 79 Reproduced from Venter, P.; Muller, M.; Vestner, J.; Stander, M.A. ; Tredoux, A.G.J.…”

Recent applications of retention modelling in liquid chromatography

“…Muller et al. tried to model the effect of the dilution factor, which is often applied to reduce the solvent strength of the 1D effluent, when coupling HILIC to RPLC [68]. The NK and the LSS model were used to calculate the retention factor at the time of elution () and the retention factor in the sample solvent ().…”

Recent applications of chemometrics in one‐ and two‐dimensional chromatography

“…No other point in the Pareto plot will provide a better resolution in a shorter analysis time. Muller et al [11] recently published methods for the kinetic optimization of HILIC × RPLC separations. They considered not only modifier gradients, but also the F I G U R E 21 Example of a Pareto plot.…”

“…Method development using one-segment-per-component optimization strategies LC 2014 [238] Optimization of HPLC with ANNs LC 2005 [216] Predictive kinetic optimization of HILIC × RP-LC separations LC × LC 2018 [11] Program for the interpretive optimization of 2D resolution LC × LC 2016 [286] Optimization of conditions 2D-RP-LC LC × LC 2015 [184] Pareto-optimality study into the comparison of LC × LC in the column and spatial mode LC × LC 2012 [296] Challenges in LC × LC Review 2018 [5] Optimizing separations in LC × LC Review 2018 [6] Optimization of GC × GC Review 2012 [182] Likelihood of total resolution in sLC × LC with parallel processing sLC × LC 2018 [297] Orthogonality Title…”

“…However, when applied to highly complex samples even with 2D chromatography, it can still be difficult to extract accurate and correct information from the obtained results. Indeed, samples such as copolymer formulations [9,10], food [11,12], protein digests [13,14], metabolic mixtures [15], and oil mixtures [16][17][18] may easily contain thousands of different components. To resolve these, powerful separation systems are needed, often equipped with sophisticated detectors such as high-resolution mass spectrometers that are able to generate huge amounts of higher-order data [19].…”