Predictive ability of reconstructed human epidermis equivalents for the assessment of skin irritation of cosmetics

Faller, Claudine; Bracher, Marguerite; Dami, Nadia; Roguet, R.

doi:10.1016/s0887-2333(02)00053-x

Cited by 118 publications

(63 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…29,35 Indeed, epidermal equivalents, such as EpiDerm, are currently employed as diagnostic models for in vitro toxicology testing. 2,9,18,17,33 In these experimental studies, IL-1a release values were comparable with those found in the current study. Furthermore, the observed tissue damage and the release of cytokines into the medium after 24 h of mechanical loading is comparable with an earlier study by Bronneberg et al, 6 indicating that reproducible results can be obtained using the current model system.…”

Section: Discussionsupporting

confidence: 90%

“…41 However, chemical exposure, UV irradiation, tape stripping, as well as sustained mechanical loading are known to disrupt the cell membrane and release a large amount of IL-1a from the keratinocytes, which normally retain prodigious intracellular stores of IL-1a in the epidermis. 2,5,6,18,19,30,32,43,44 It was shown that mechanical straining of keratinocyte monolayers promotes the expression and release of IL-1a and IL-1RA. 25,40 Recently, IL-1a, IL-1RA, IL-8, and TNF-a have been identified as promising markers for pressure ulcer risk detection.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Transport Profile of Cytokines in Epidermal Equivalents Subjected to Mechanical Loading

et al. 2009

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

The Transport Profile of Cytokines in Epidermal Equivalents Subjected to Mechanical Loading

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Although the induced irritation can be reversible in some cases, there are others in which it can evolve into permanent damage. Reconstructed models of human epidermis have been used to mimic human skin and then assess the tolerance of human skin to potential irritants in cosmetic formulations [65,66]. In Figure 7, we can observe that the viability of keratinocytes was not significantly affected by the presence of codfish collagen.…”

Section: Effect Over Viability Of Keratinocytes On Reconstructed Skinmentioning

confidence: 99%

Cosmetic Potential of Marine Fish Skin Collagen

et al. 2017

View full text Add to dashboard Cite

Many cosmetic formulations have collagen as a major component because of its significant benefits as a natural humectant and moisturizer. This industry is constantly looking for innovative, sustainable, and truly efficacious products, so marine collagen based formulations are arising as promising alternatives. A solid description and characterization of this protein is fundamental to guarantee the highest quality of each batch. In the present study, we present an extensive characterization of marine-derived collagen extracted from salmon and codfish skins, targeting its inclusion as component in cosmetic formulations. Chemical and physical characterizations were performed using several techniques such as sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), Fourier Transformation Infrared (FTIR) spectroscopy rheology, circular dichroism, X-ray diffraction, humidity uptake, and a biological assessment of the extracts regarding their irritant potential. The results showed an isolation of type I collagen with high purity but with some structural and chemical differences between sources. Collagen demonstrated a good capacity to retain water, thus being suitable for dermal applications as a moisturizer. A topical exposure of collagen in a human reconstructed dermis, as well as the analysis of molecular markers for irritation and inflammation, exhibited no irritant potential. Thus, the isolation of collagen from fish skins for inclusion in dermocosmetic applications may constitute a sustainable and low-cost platform for the biotechnological valorization of fish by-products.

show abstract

“…The tissue exhibits organized basal, spinous and granular layers, and a multi-layered stratum corneum containing intercellular lamellar lipid layers arranged in patterns analogous to those found in vivo (Cannon et al, 1994). Because it closely mimics the human epidermis, it has proven its scientific relevancy in studies where dermal exposure and toxicity is anticipated: skin corrosion testing of chemicals, skin irritation studies of cosmetic products and raw materials, phototoxicity, and skin penetration studies (Liebsch et al, 1997(Liebsch et al, , 2000Faller et al, 2002;Schäfer-Korting et al, 2008). Cell viability was measured by the MTT reduction assay and MTT Effective Time 50 (ET50) was determined after topical application of the products for different exposure times (2, 5, and 18 h), according to manufacturer's instructions.…”

Section: Reconstructed Human Skin Modelmentioning

confidence: 99%

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

Letsiou¹,

Kalliampakou

Gardikis³

et al. 2017

Front. Mar. Sci.

View full text Add to dashboard Cite

Nowadays, there is huge interest in natural products obtained from marine organisms that can promote a state of health and well-being for humans. Microalgae represent a primary source of bioactive compounds that could be used as functional ingredients in cosmetic formulations. The aim of the present study is to evaluate, for the first time, the effects of Nannochloropsis gaditana extract against oxidative stress in human primary fibroblasts so as to investigate the potential applications of it in cosmetics. To gain an insight into the molecular mechanisms of N. gaditana bioactivity, we developed a new RT-qPCR platform for studying transcript accumulation for an array of selected genes (up to 100) involved in many skin-related processes including anti-aging, hydration, oxidative stress response, and DNA damage. For the oxidative stress evaluation, H 2 O 2 was used as a stressor. The study of the transcript accumulation of genes revealed that N. gaditana extract exhibits skin protection properties by mediating oxidative responses and apoptosis (including SOD1, GPX1, BID), positively regulates genes involves in skin texture and hydration (including AQP3, Col6A1, FBN1) and modulates the expression of genes involved in skin irritation, DNA damage and aging (including IL1R, PCNA, FOXO3). These findings indicate that the specific N. gaditana extract possesses significant in vitro skin protection activity against induced oxidative stress, and provide new insights into the beneficial role of microalgae bioactive compounds in cosmetic formulations protecting skin from oxidative stress.

show abstract

Predictive ability of reconstructed human epidermis equivalents for the assessment of skin irritation of cosmetics

Cited by 118 publications

References 24 publications

The Transport Profile of Cytokines in Epidermal Equivalents Subjected to Mechanical Loading

The Transport Profile of Cytokines in Epidermal Equivalents Subjected to Mechanical Loading

Cosmetic Potential of Marine Fish Skin Collagen

Skin Protective Effects of Nannochloropsis gaditana Extract on H2O2-Stressed Human Dermal Fibroblasts

Contact Info

Product

Resources

About