Prediction of total body lipid from total body water in rats. Part 1. Relations between directly measured major body components

Zahn, Laura M.; Freund, Rudolf J.; Hoppe, Karl; Pisarchuk, K. L.; Augustin, Wolfgang

doi:10.1002/food.19910350605

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…Rozman and Klaassen (1995) contended that the concentration of a toxicant in blood depends largely on its volume of distribution, indicating that body water volume could influence the measured toxin concentrations and may have been a factor in the higher blood concentration of some alkaloids in these animals. It is unlikely however, that body water volume affected this study because body water increases as body fat decreases (Scholz et al, 1990;Zahn et al, 1991), and thinner ewes had higher serum concentrations of alkaloids than did fatter ewes. Foley et al (1995) proposed that toxin breakdown during detoxification requires energy and substrates for biotransformation and elimination.…”

Section: Discussionmentioning

confidence: 95%

The effect of body condition on disposition of alkaloids from silvery lupine (Lupinus argenteus Pursh) in sheep1

López-Ortíz

Panter

Pfister³

et al. 2004

Journal of Animal Science

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Several species of lupine (Lupinus spp.) are poisonous to livestock, producing death in sheep and "crooked calf disease" in cattle. Range livestock cope with poisonous plants through learned foraging strategies or mechanisms affecting toxicant disposition. When a toxic plant is eaten, toxicant clearance may be influenced by the animal's nutritional and/or physiological status. This research was conducted to determine whether differences in body condition or short-term nutritional supplementation of sheep altered the disposition of lupine alkaloids given as a single oral dose of ground silvery lupine (Lupinus argenteus) seed. Ewes in average body condition (ABC, n = 9) and low body condition (LBC, n = 10) received a single dose of ground lupine seeds including pods (8.5 g/kg BW) via gavage on the first day of the experiment, and were then randomly assigned to one of two nutritional supplement treatments. Blood samples were taken 0 to 60 h after dosing to compare blood alkaloid concentration and to evaluate alkaloid absorption and elimination profiles. Concentrations of total alkaloid and anagyrine, 5,6 dehydrolupanine, lupanine, and alkaloid E were measured in serum. These four alkaloids constituted 78 and 75% of the total alkaloid concentration in serum for LBC vs. ABC groups, respectively. Initial analysis indicated that short-term supplementation had no effect on alkaloid disposition, and supplementation was removed from the statistical model. The highest concentration of total alkaloids was observed 2 h after dosing. Overall, serum total alkaloid and anagyrine levels (area under the curve) were higher (P < 0.01) for sheep in the LBC group. Serum peak concentrations of total alkaloid and anagyrine were higher in LBC vs. ABC groups (P < 0.05). Serum elimination of anagyrine, unknown alkaloid E, and lupanine was decreased in LBC vs. ABC treatments (P < 0.05). These results demonstrate that body condition is important in the disposition of lupine alkaloids; however, further research is needed to determine the potential benefit, if any, that short-term nutritional supplementation might have on alkaloid disposition.

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Section: Discussionmentioning

confidence: 95%

The effect of body condition on disposition of alkaloids from silvery lupine (Lupinus argenteus Pursh) in sheep1

López-Ortíz

Panter

Pfister³

et al. 2004

Journal of Animal Science

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Prediction of total body lipid from total body water in rats. Part 2. In vivo estimation of total body lipid by tritium water dilution

Zahn

Freund

Noack

et al. 1991

Nahrung

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Body composition data estimated for normal young, normal adult, and adult obese Wistar rats by a tritiated water (THO) dilution procedure (b. wt. range 119-237 g, n = 31; 305-597 g, n = 43; 274-381 g, n = 6) were compared with the results of whole body analysis. As for the predicted total body lipid (TBL), the content was calculated from the virtual THO-dilution space estimated by a simple micro-method and a regression equation between TBL [%] and DM [%] (dry matter) content (Part 1. Relations between directly measured major body components. Nahrung 35 (1991) 581. Due to different degrees of overestimations in the animal groups examined, the THO-space can be corrected via subtraction of 3.8%, 2.2% and 1.1% in the sequence young, obese, and adult groups. The derived TBL [%] data show a non-significant deviation of less than 1 g/100 g b. wt. in comparison to the three group means after direct lipid determinations. In the best case, i. e. after the additional elimination of extremely overestimated values for total body water (outliers) the combined tritium-derived lipid values (y) are correlated with the lipid data obtained analytically (x), both as proportion of the body weight, by the regression equation y = 0.848 + 0.963 x, r = 0.985, Sy.x = 1.77, n = 58. For a group of young rats (n = 14) after a pre-experimental supplementation with a sucrose solution, TBL values, not significantly different from analytically determined ones, could be calculated without any corrections.

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Prediction of total body lipid from total body water in rats. Part 1. Relations between directly measured major body components

Cited by 2 publications

References 23 publications

The effect of body condition on disposition of alkaloids from silvery lupine (Lupinus argenteus Pursh) in sheep1

The effect of body condition on disposition of alkaloids from silvery lupine (Lupinus argenteus Pursh) in sheep1

Prediction of total body lipid from total body water in rats. Part 2. In vivo estimation of total body lipid by tritium water dilution

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