Prediction of the optimal time for insemination using frozen-thawed semen in a multi-sire insemination trial in bitches

Steckler, Daniela; Nöthling, J.O.; Harper, Cindy Kim

doi:10.1016/j.anireprosci.2013.09.013

Cited by 17 publications

(20 citation statements)

References 19 publications

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“…The levels of progesterone and cortisol were determined in each plasma sample, using the Coat-A-Count® radioimmunoassay (Siemens Health Care Diagnostics Inc. Los Angeles, CA 90045 USA) for each hormone. This assay has been validated for dog plasma [22] and used to determine the progesterone level (PL or PLs for progesterone levels) in the serum or plasma of bitches for decades [23][24][25][26][27][28] and for cortisol assays [29][30][31].…”

Section: Collection Of Specimens and Datamentioning

confidence: 99%

The precision of predicting the time of onset of parturition in the bitch using the level of progesterone in plasma during the preparturient period

Cramer

Nöthling

2018

Theriogenology

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Precise prediction of the time of onset of parturition in the bitch is of clinical importance. Many parturition management cases in clinical practice are presented in the last two weeks of pregnancy without a reliable estimate of the parturition date. The aim of this study was to assess the value of a single progesterone level in the blood plasma from a preparturient bitch to predict the time of onset of parturition. The temporal relationship between the decrease in the plasma progesterone levels and the time of cervical dilatation (TCD)-which correlates to the onset of stage 1 of parturition-was evaluated in 25 bitches in the preparturient period. Among bitches destined to reach TCD within 12 h there is a 2% probability of having a plasma progesterone level of 15.8 nmoL/L or above and a 6% probability of having a level of 8.7 nmoL/L or above. Conversely, if the level is below 8.7 nmoL/L there is a 99% probability of reaching TCD within 48 h and if the level is below 3.18 nmoL/L there is a 100% probability of reaching TCD within 24 h. These results allow the veterinary obstetrician to make prompt decisions in the management of parturition.

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Section: Collection Of Specimens and Datamentioning

confidence: 99%

The precision of predicting the time of onset of parturition in the bitch using the level of progesterone in plasma during the preparturient period

Cramer

Nöthling

2018

Theriogenology

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“…; Steckler et al. ). Pregnancy length from the day of ovulation has been described to range 62–64 days (Concannon et al.…”

Section: Introductionmentioning

confidence: 98%

Laboratory and Clinical Evaluation of a Feia Method for Canine Serum Progesterone Assay

Rota

Vannozzi

Marianelli

et al. 2015

Reprod Domestic Animals

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The evaluation of progesterone (P4) concentration is a valuable tool in assessing physiological reproductive events and reproductive disorders in bitches. A reliable and rapid (preferable, point of care) determination of P4 is advisable in most cases. Aims of this study were to evaluate a fluorescence enzyme immunoassay (FEIA) for canine serum P4 concentration by (i) the agreement with liquid chromatography-tandem mass spectrometry (LC/MS/MS), (ii) the association with vaginal cytology and (iii) the accuracy in the prediction of the parturition date calculated from the estimated day of ovulation. Serum samples were collected from client-owned bitches presented between 2011 and 2014 for the evaluation of their oestrous cycle, pregnancy or reproductive disorders. The agreement between FEIA and LC/MS/MS, evaluated on 19 samples, was statistically significant (R(2) = 95.7%, p < 0.001), although FEIA showed significantly higher values than LC/MS/MS (p < 0.05). In the different phases of oestrous cycle, as determined by vaginal cytology, P4 concentrations (by FEIA) were statistically different (p < 0.05): anoestrus (n = 7) 0.38 ± 0.14 ng/ml, proestrus (n = 14) 1.04 ± 0.67 ng/ml and oestrus (n = 72) 6.8 ± 7.26 ng/ml. Mean pregnancy length from the estimated day of ovulation was 62.9 ± 1.8 days. In 13 of 22 (59.1%), 19 of 22 (86.3%) and 21 of 22 (95.5%) bitches pregnancy lasted 63 ± 1, 63 ± 2 and 63 ± 3 days, respectively. Three pregnancies were outside the 61-65 days range (60, 60 and 67 days). In conclusion, the FEIA method employed can be considered reliable and, in association with vaginal cytology, effective in evaluating the canine oestrous cycle.

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“…Ovariohysterectomy of the pregnant bitch enables the researcher to precisely and accurately determine the number of corpora lutea and post-implantation conceptuses [2,3,[11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Estimated impact of multiple conceptuses per follicle on fecundity in the bitch

Steckler

Cramer²,

Nöthling

2017

Theriogenology

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Multiple conceptuses from one follicle due to multi-ovular follicles or monozygotic twins inflate the ratio between the numbers of conceptuses and corpora lutea and possibly the effect ascribed to experimental treatment. The aim of this study was to estimate the impact of multiple conceptuses per follicle on fecundity in the bitch. The numbers of conceptuses and corpora lutea of 105 bitches originated from 7 published experimental studies and of 132 from ovariohysterectomies done in a private practice and a welfare organisation. Ninety five bitches, producing 597 conceptuses, had at least as many conceptuses as corpora lutea; 83 had as many, 10 had one more and 2 had 2 more. Bootstrap resampling on these 95 bitches let us to conclude that 12.6% of bitches are expected to produce more oocytes that are each capable of yielding a conceptus than the number of follicles that ovulate and that 2.35% of conceptuses are expected to be in excess of the number of follicles that ovulate. Applying the results of the current study to 2 earlier studies showed that multiple conceptuses from one follicle may, at maximum, have increased the effect ascribed to prostatic fluid by 0.022 and 0.024 conceptuses per corpus luteum, which is minor in relation to the magnitude of the ascribed effects of 0.34 and 0.22 conceptuses per corpus luteum. The number of conceptuses relative to the number of corpora lutea provides a valid and precise measurement of fertility in the bitch.

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Prediction of the optimal time for insemination using frozen-thawed semen in a multi-sire insemination trial in bitches

Cited by 17 publications

References 19 publications

The precision of predicting the time of onset of parturition in the bitch using the level of progesterone in plasma during the preparturient period

The precision of predicting the time of onset of parturition in the bitch using the level of progesterone in plasma during the preparturient period

Laboratory and Clinical Evaluation of a Feia Method for Canine Serum Progesterone Assay

Estimated impact of multiple conceptuses per follicle on fecundity in the bitch

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