Prediction of the digestibility of ruminant feeds from their solubility in enzyme solutions

Clark, Jennifer; Beard, Janet

doi:10.1016/0377-8401(77)90016-5

Cited by 16 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inoculum from cattle fed barley-dehy resulted in the least variation (r=0.88; Sy.x ± 2.49; P<O.Ol ). The standard error of this relationship is smaller than that of relationships for predicting forage digestibility from chemical composition or solubility in enzyme solutions (Minson et al, 1976;Clark and Beard, 1977).…”

Section: Resultsmentioning

confidence: 99%

In Vitro Fermentation of Forages with Inoculum from Cattle and Sheep Fed Different Diets¹

Horton¹,

Christensen²,

Steacy

1980

Agronomy Journal

View full text Add to dashboard Cite

Inoculum is the single most important source of variation in in vitro rumen fermentation systems used to predict forage quality. This investigation was carried out to study the effects of donor animal diet and donor animal species on the activity of the inoculum (whole rumen fluid) used in the two stage in vitro fermentation technique for estimating forage digestibility. Two wethers and two steers were fed either wheat straw (Triticum aestivum L.) smooth bromegrass (Bromus inermis Leyss.) alfalfa (Medicago sativa L.) hay (hay) or barley (Hordeum vulgare L.) and dehydrated alfalfa (1:l) pellets (barleydehy) in a double switchback arrangement with three periods of 28 days. Nineteen forages including alfalfa, smooth bromegrass, crested wheatgrass [Agropyron desertorurm (Fisch ex Link) Schult.] and oat (Avena sativa L.), wheat and barley straws were digested in vitro and in vivo by steers. Each substrate was digested in duplicate during three successive periods with inoculum from sheep and cattle consuming each of the three diets. Donor diet had a significant effect on in vitro dry matter (IVDMD) and acid‐detergent fiber digestibility (IVADFD) with inoculum from sheep and cattle. Values were lowest (P<0.001) with inoculum from animals fed straw and were highest when hay was fed. In vitro digestibilities were higher (P<0.001) with sheep inoculum than with cattle inoculum on straw and barley‐dehy diets; values were highest (P<0.001) with cattle inoculum on the hay diet. Estimates of in vivo digestibility using IVADFD were not significant. Linear regression equations, correlations, and standard errors showed a significant relationship (P<0.01) between in vivo dry matter digestibility and IVDMD for inoculum from both sheep and cattle fed either straw, hay, or barley‐dehy. The best fit of cattle in vivo dry matter digestibility was to IVDMD using inoculum from cattle fed the pelleted barley‐dehy diet (r=0.88; Sy.x, ± 2 2.49; P<0.01).

show abstract

Section: Resultsmentioning

confidence: 99%

In Vitro Fermentation of Forages with Inoculum from Cattle and Sheep Fed Different Diets¹

Horton¹,

Christensen²,

Steacy

1980

Agronomy Journal

View full text Add to dashboard Cite

show abstract

“…pH 4.5 40 °C 72 h 70 mg pepsin in 1 mL of 1 M acetate. pH 4.5 40 °C 24 h 0.80 – Rumen jus [ 2 ] HCl 0.1 N–2 g L −1 pepsin [ 2 ] 0.95 – [ 35 ] Adegbola and Paladines (1977) Tropical grassesLegumes Sheep HCl 0.05 NPepsin 48 h 40 °C Cellulase Trichoderma (Worthinton Biochemical Corporation) 48 h 40 °C 0.98 – [ 36 ] Clark and Beard (1977) Green SilageHaysStraw Sheep or cattle HCl 0.1 NPepsin 24 h 40 °C Cellulase ( Aspergillus niger ) 48 h 40 °C 0.80 y = 39.1 + 0.76x [ 37 ] Dowman and Collins (1977) Grass silage Sheep HCl 0.1 NPepsin 24 h 40 °C Cellulase “BDH” 48 h 40 °C 0.89 y = 0.58x + 31.57 [ 38 ] Goto and Minson (1977) Tropical and temperate grasses Sheep HCl 0.1 NPepsin 48 h 40 °C Cellulase “Onozuka SS—P” 48 h 40 °C 0.94 y = 0.69x + 20.3 [ 39 ] Roughan and Holland (1977) Temperate grasses and legumes – NDF solution Cellulase Trichoderma viride 5 h and 18 h 50 °C 0.98 …”

Section: Introductionmentioning

confidence: 99%

High throughput accurate method for estimating in vitro dry matter digestibility of maize silage

et al. 2021

View full text Add to dashboard Cite

Background Since the introduction of studies on maize silage digestibility at the end of the nineteenth century, protocols to estimate dry matter digestibility have not stopped evolving. Since the early 1980s, the protocol developed by Aufrère became a benchmark in many laboratories to estimate in vitro dry matter digestibility. In order to increase its throughput, to facilitate its execution and to decipher the impact of the different parameters of the protocol we decided to test the combination of 7 parameters in 21 different protocols. Results We thus tested the impact of (1) the presence or absence of pepsin in HCl solution, (2) the temperature of incubation during enzymatic hydrolysis, (3) the presence or absence of a gelatinization step, (4) washing/rinsing versus neutralization step, (5) the presence or absence of α-amyloglucosidase in enzymatic solution, (6) the duration of cellulase incubation, and (7) the concentration of the cellulase solution. The major result of our work highlighted that it was essential to carry out a gelatinization step to correctly estimate the in vitro dry matter digestibility of maize silage. Conclusions The proposed protocol in this paper is innovative, reliable, highthroughput and easy to implement in many laboratories to accurately quantity in vitro dry matter digestibility.

show abstract

“…Fungal cellulase methods have potential for predicting in vivo dry matter digestibility (DMD) of forages with great accuracy. Clark and Beard (1977) reported that a fungal cellulase digestion procedure showed less variation than in vitro DMD using rumen liquor. In addition, cellulase digestion procedures allow researchers who are unable to maintain donor animals the opportunity to obtain good estimates ofDMD inexpensively.…”

mentioning

confidence: 99%

Digestion of Several Temperate Forage Species by a Prepared Cellulase Solution¹

Bughrara¹,

Sleper

1986

Agronomy Journal

View full text Add to dashboard Cite

Rapid, inexpensive, and accurate procedures are required for predicting in vivo dry matter digestibility (DMD) offorages, particularly in a forage breeding program when many samples may be analyzed simultaneously. The primary objective of this study was to investigate the relationships of several cellulase digestion procedures to in vivo DMD, in vitro DMD using rumen liquor, neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and hemicellulose for several temperate forage species. A cellulase solution was produced by culturing the conidia from Trichoderma reesei Simmons in a mineral medium containing 10 g L -• cellulose. Apparent activity of the cellulase solution was determined by digesting cool season forages of known DMD and solka floc substrate. Fortytwo dried cool season forages which previously had been evaluated for per cent NDF, ADF, ADL, in vitro DMD using rumen liquor, and in vivo DMD using sheep were subjected to different cellulase digestion procedures. Correlations between the various cellulase DMD techniques and in vivo DMD were highly significant (p < 0.01) and ranged from 0.89 to 0.97. Correlations of the cellulase in vitro DMD techniques and NDF, ADF, ADL, and hemicellulose were negative and similar in magnitude to the same parameters ,when correlated to in vitro DMD using rumen liquor. The data suggested that the in vitro DMD obtained by using a prepared cellulase solution was an excellent predictor of in vivo DMD and the digestion was similar to that when digesting forages with rumen liquor. AdditioiUII index words: Trichoderma reesei Simmons, Orchardgrass (Dactylis glomerata L.), Smooth bromegrass (Bromus inermisLeyss.), Timothy (Phleum pratense L.), Alfalfa (Medicago sativa L.), In vivo dry matter digestibility, In vitro dry matter digestibility, Neutral detergent fiber, Acid detergent fiber, Acid detergent lignin, Hemicellulose.~~ontribution from the Missouri Agric. Exp. Stn., Journal S1:ries no. 9785.

show abstract

Prediction of the digestibility of ruminant feeds from their solubility in enzyme solutions

Cited by 16 publications

References 8 publications

In Vitro Fermentation of Forages with Inoculum from Cattle and Sheep Fed Different Diets¹

In Vitro Fermentation of Forages with Inoculum from Cattle and Sheep Fed Different Diets¹

High throughput accurate method for estimating in vitro dry matter digestibility of maize silage

Digestion of Several Temperate Forage Species by a Prepared Cellulase Solution¹

Contact Info

Product

Resources

About

Prediction of the digestibility of ruminant feeds from their solubility in enzyme solutions

Cited by 16 publications

References 8 publications

In Vitro Fermentation of Forages with Inoculum from Cattle and Sheep Fed Different Diets1

In Vitro Fermentation of Forages with Inoculum from Cattle and Sheep Fed Different Diets1

High throughput accurate method for estimating in vitro dry matter digestibility of maize silage

Digestion of Several Temperate Forage Species by a Prepared Cellulase Solution1

Contact Info

Product

Resources

About

In Vitro Fermentation of Forages with Inoculum from Cattle and Sheep Fed Different Diets¹

In Vitro Fermentation of Forages with Inoculum from Cattle and Sheep Fed Different Diets¹

Digestion of Several Temperate Forage Species by a Prepared Cellulase Solution¹