Prediction of the Coding Sequences of Unidentified Human Genes. XIV. The Complete Sequences of 100 New cDNA Clones from Brain Which Code for Large Proteins in vitro

Abstract

To extend our cDNA project for accumulating basic information on unidentified human genes, we newly determined the sequences of 100 cDNA clones from a set of size-fractionated human adult and fetal brain cDNA libraries, and predicted the coding sequences of the corresponding genes, named KIAA1019 to KIAA1118. The sequencing of these clones revealed that the average size of the inserts and corresponding open reading frames were 5.0 kb and 2.6 kb (880 amino acid residues), respectively. Database search of the predicted amino acid sequences classified 58 predicted gene products into the five functional categories, such as cell signaling/communication, cell structure/motility, nucleic acid management, protein management and cell division. It was also found that, for 34 gene products, homologues were detected in the databases, which were similar in sequence through almost the entire regions. The chromosomal locations of the genes were determined by using human-rodent hybrid panels unless their mapping data were already available in the public databases. The expression profiles of all the genes among 10 human tissues, 8 brain regions (amygdala, corpus callosum, cerebellum, caudate nucleus, hippocampus, substania nigra, subthalamic nucleus, and thalamus), spinal cord, fetal brain and fetal liver were also examined by reverse transcription-coupled polymerase chain reaction, products of which were quantified by enzyme-linked immunosorbent assay.

PDZRN3 (LNX3, SEMCAP3) is required for the differentiation of C2C12 myoblasts into myotubes

“…The final three amino acids at the C-terminus of the protein encoded by the insert were found to be Thr-Thr-Val, which match the consensusbinding motif for class I PDZ domains. A search of the GenBank database revealed that the insert corresponded to the partial sequence of KIAA1095, which was isolated in a random cloning strategy (Kikuno et al, 1999). The predicted protein PDZRN3 contains a RING-finger motif in its N-terminal region, two PDZ domains in its central region and a consensus-binding motif for PDZ domains at its Cterminus.…”

Development of cDNA microarray for expression profiling of estrogen-responsive genes

“…2·1 (Takara Shuzo, Otsu, Japan). The sequences of the primers used in PCR amplification were as follows: 5 -GAG GCA ATG GCC ACC ATG G-3 and 5 -GTA GTC AAA GTC AGA GCA GTC-3 for pS2 (Jakowlew et al 1984); 5 -GTG GGG GCA AGA TGA AGG TC-3 and 5 -TTA CCC CAA GGG CAC ACC C-3 for insulin-like growth factor (IGF)-binding protein-4 (IGFBP4) (Kiefer et al 1991b); 5 -TTC GAG AGC AAG TGG AAC CC-3 and 5 -AGC TCC TCC TGA ATG TGG TC-3 for KIAA1051 (Kikuno et al 1999); 5 -AGC TGT GGA AGC CCT AAC TC-3 and 5 -TCG TAG CCG GTT AAC GCC AG-3 for retinoblastoma-binding protein 8 (Fusco et al 1998); 5 -AAT GGC GGT TCT CAT GCT GG-3 and 5 -ATC TGG TTG ACT TTG AGC AGG-3 for c-myc promoterbinding protein 1 (Ray & Miller 1991); 5 -ACG AAA AGA GCT ACC GCG AG-3 and 5 -TTG CTG CTG TCG AAG GTG TG-3 for insulin-like growth factor-binding protein-5 (IGFBP5) (Kiefer et al 1991a); 5 -TGG AGG CAC GGA CCA CTG C-3 and 5 -AGA CAG TCC CCT GCG GTG G-3 for solute carrier family 7 member 5 (Gaugitsch et al 1992); 5 -GCA CAG AGC CTC GCC TTT G and 5 -CAT CAC GAT GCC AGT GGT A-3 for -actin (Nakajima-Iijima et al 1985). Twenty-five nanograms of cDNA fragments were labeled with [ -32 P]dCTP using Megaprime DNA labeling systems (Amersham Pharmacia Biotech), and hybridization was carried out using ExpressHyb Hybridization solution (Clontech) according to the manufacturer's instructions.…”

Expression and significance of the imprinted gene PEG10 in placenta of patients with preeclampsia

“…Kikuno et al (1999) cloned it in 1999, which is located on human chromosome 7q21 and mouse chromosome 6A1. PEG10 encodes at least two proteins such as PEG10 RF1 and PEG10 RF1/2 (Clark et al, 2007).…”