Prediction of the Coding Sequences of Unidentified Human Genes. XVII. The Complete Sequences of 100 New cDNA Clones from Brain Which Code for Large Proteins in vitro

Abstract

To provide information regarding the coding sequences of unidentified human genes, we have conducted a sequencing project of human cDNAs which encode large proteins. We herein present the entire sequences of 100 cDNA clones of unknown human genes, named KIAA1444 to KIAA1543, from two sets of size-fractionated human adult and fetal brain cDNA libraries. The average sizes of the inserts and corresponding open reading frames of cDNA clones analyzed here were 4.4 kb and 2.6 kb (856 amino acid residues), respectively. Database searches of the predicted amino acid sequences classified 53 predicted gene products into the following five functional categories: cell signaling/communication, nucleic acid management, cell structure/motility, protein management and metabolism. It was also revealed that homologues for 32 KIAA gene products were detected in the databases, which were similar in sequence through almost their entire regions. Additionally, the chromosomal loci of the genes were determined by using human-rodent hybrid panels unless their chromosomal loci were already assigned in the public databases. The expression levels of the genes were monitored in spinal cord, fetal brain and fetal liver, as well as in 10 human tissues and 8 brain regions, by reverse transcription-coupled polymerase chain reaction, products of which were quantified by enzyme-linked immunosorbent assay.

Genetic variation in the 22q11 locus and susceptibility to schizophrenia

“…2c): the DGCR8 gene (which encodes for a protein of undetermined function), the FLJ22127 gene (which encodes for a protein with a double-strand RNA-binding domain), the HTF9C gene (28) (which likely encodes for a methyltransferase), and the RANBP1 gene (29) (which encodes for a protein that interacts with the cell-cycle regulatory protein Ran͞TC4). Two more genes predicted by analysis of the syntenic region on mouse chromosome 16 and supported by available EST and mRNA databases are located either within BLOCK I (KIAA1292) (30) or immediately outside it (T10) (31) (Fig. 2c).…”

Interaction of folliculin (Birt-Hogg-Dubé gene product) with a novel Fnip1-like (FnipL/Fnip2) protein

“…KIAA1450 was originally identified in a cDNAsequencing project carried out by the Kazusa DNA Research Institute (Nagase et al, 2000). KIAA1450 is localized on human chromosome 4q, whereas FNIP1 is on chromosome 5q (HUGE data base, Kazasa DNA Research Institute; http://www.kazusa.or.jp/huge/) (Nagase et al, 2001).…”

Cross GTPase-activating protein (CrossGAP)/Vilse links the Roundabout receptor to Rac to regulate midline repulsion

“…CrGAP encodes a protein with at least one highly conserved homologue in human and in mouse. The human homologue of CrGAP, KIAA1688, was identified as a cDNA with large protein product from the brain (22), and shares 54.4% sequence similarity with Drosophila CrGAP. The gene contains at least three highly conserved signaling motifs: two WW domains, a MyTh4 (myosin tail homology) domain, and a Rho GAP domain (Fig.…”