Prediction of the best signal peptide for periplasmic expression of melittin peptide in Escherichia coli
Amir Asghari Baghkheirati,
Elnaz Karbaschian,
Seyedah Zahra Mousavi
et al.
Abstract:Periplasmic expression of antimicrobial peptides is one of the most important issues in cloning and protein expression systems. In the present study, bioinformatics methods were used to predict the best signal peptides for periplasmic expression of the melittin peptide in Escherichia coli. Therefore, the sequence of 12 signal peptides was prepared from signal peptide databases. In order to choose the best signal peptide, periplasmic expression, sub-cellular localization site, solubility, physical, and chemical… Show more
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