Prediction of porcine semen fertility by homologous in vitro penetration (hIVP) assay

Gadea, J.; Matás, Carmen; Lucas, Xiomara

doi:10.1016/s0378-4320(98)00144-4

Cited by 71 publications

(60 citation statements)

References 23 publications

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“…For example, in a field trial with three boars of known different in vivo fertilizing capacity (low, intermediate, and high), the sperm penetration rate and the number of spermatozoa differed significantly between the groups, with the lowest numbers in the low-fertility boar, and the highest numbers in the high-fertility boar [Gadea et al 1998]. Gil et al [2005] performed an IVF study on frozen-thawed boar semen, in which they divided the boars into two groups, ''good'' or ''bad'' boars, according to the freezability of their semen (with regard to, e.g., motility, motion characteristics and sperm viability post-thaw).…”

Section: Resultsmentioning

confidence: 99%

Pre-pubertal Di(2-ethylhexyl) Phthalate (DEHP) Exposure of Young Boars Did Not Affect SpermIn vitroPenetration Capacity of Homologous Oocytes Post-puberty

Spjuth

Gil

Caballero

et al. 2007

Archives of Andrology

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Di(2-ethylhexyl) phthalate (DEHP), a plastic softener used in polyvinylchloride (PVC) products (e.g., plastic bags and medical equipment), has been reported to have toxic effects on animal reproduction and is considered an environmental hazard based, mostly, on rodent studies. However, the doses used in these studies are often considerably higher than that presumed in human exposure. In the present study we used young boars as model animals to assess the effects of pre-pubertal DEHP exposure on the ability of spermatozoa to penetrate homologous oocytes in vitro. Eight pairs of cross-bred male boar siblings were used. One brother in each pair became, at random, the test animal exposed to DEHP per os, three times a week, from 3 to 7 weeks of age while the other acted as the control, i.e., placebo-exposed. Semen was collected and frozen between 8 and 9 months of age and stored until spermatozoa were evaluated for their ability to in vitro penetrate in vitro-matured homologous oocytes post-thaw. Both the penetration rate and the number of spermatozoa per oocyte were considered within expected ranges for frozen boar semen of good quality. Penetration rate did not significantly differ (p > 0.05) between the groups with DEHP-exposed: 50%; control: 59%, which could be owing to a large variation between boars, and between replicates. The number of spermatozoa in the ooplasm was low and similar (p > 0.05) between the groups with DEHP-exposed: 1.5 and the control: 1.7. Under the conditions of the present experiment, pre-pubertal exposure to DEHP does not seem to cause a deleterious effect on the in vitro fertilizing ability of frozen spermatozoa post-puberty.

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Section: Resultsmentioning

confidence: 99%

Pre-pubertal Di(2-ethylhexyl) Phthalate (DEHP) Exposure of Young Boars Did Not Affect SpermIn vitroPenetration Capacity of Homologous Oocytes Post-puberty

Spjuth

Gil

Caballero

et al. 2007

Archives of Andrology

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“…Several authors have emphasized the suitability of the HOS test for assessing the quality of human semen (Moskovtsev et al, 2005;Cincik et al, 2007) and also that of various domestic animals including cattle (Correa and Zavos, 1994;Mandal et al, 2003;Hu et al, 2010), horses (Neild et al, 1999), and pigs (Gadea et al, 1998). As the test gives a consistent estimate of the percentage of spermatozoa with a physiologically active membrane, it can be used to predict the fertilizing capacity of spermatozoa in animals (Rota et al, 2000;Brito et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls

Padrik¹,

Hallap²,

Kaart³

et al. 2012

CZECH J ANIM SCI

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ABSTRACT:As an attempt to find an inexpensive and simple laboratory method for artificial insemination (AI) bull semen quality assessment, the osmotic resistance of spermatozoa was measured using the hypoosmotic swelling (HOS) test, developed by Jeyendran et al. (1984) (labelled HOS-1), and its modifications (HOS-2, HOS-3), with decreased osmotic pressure aimed at challenging sperm survival ability. The test results were benchmarked against sperm viability measurements performed using the Computerized Motility Analyzer (CMA), and field fertility was calculated as non-return rate (NRR). Two age groups of Estonian Holstein bull sires were included in this study to test possible age effect on semen quality parameters. The HOS-1 test in fresh bull semen correlated well with sperm general motility (GMot) (r = 0.63, P < 0.001 at batch level and r = 0.77, P < 0.001 at bull level) as well as with progressive motility (PMot) in frozen-thawed (FT) semen (r = 66, P < 0.001 at batch level and r = 0.81, P < 0.001 at bull level), which makes the test suitable for the prediction of post-thaw semen quality. However, the HOS-2 and HOS-3 values in FT semen had high correlations with NRR (r = 0.65, r = 0.66, P < 0.001 at batch level and r = 0.63, r = 0.71, P < 0.01 at bull level), which was comparable to those between GMot and NRR or PMot and NRR. A combination of motility parameters and the results of the HOS-1 and HOS-3 tests provided a good model for predicting the potential fertility of bull semen. Values of sperm membrane post-thaw intactness, assessed using HOS-2, as well as of sperm motility measurements were higher in mature bulls compared to those in young bulls. Short conclusion: different modifications of the hypo-osmotic swelling test are useful for routine bovine semen quality assessment at AI stations.Keywords: bull fertility; semen quality; sperm membrane intactness; bull age Laboratory analyses have been used over many decades to evaluate semen quality. Despite the fact that none of the single assays developed provide results that consistently and highly correlate with fertility, the laboratory evaluation of semen samples remains an important procedure for the artificial insemination (AI) industry to eliminate low fertility bulls or semen from being used in artificial insemination programmes (Graham and Mocé, 2005).Post-thaw viability and fertility of cryopreserved sperm are often reduced due to accumulated cellular damage during the cryopreservation phases (Muiño et al., 2008). Decrease in temperature, cold shock, and intracellular ice formation can affect sperm plasma membrane, acrosomal and mitochondrial membrane integrity (Thomas et al., 1998;Defoin et al., 2008), and cause the loss of intracellular components (Graham and Mocé, 2005), which can initiate cell death.Sperm membrane integrity can be evaluated by several methods, e.g. light or fluorescent microscopy combined with vital stains (Brito et al., 2003), and flow cytometry (Januskauskas et al.,

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“…Testes que incluem a interação dos gametas masculino e feminino, tais como o de ligação à zona pelúcida (Ivanova & Mollova, 1993;Fazelli et al, 1995;Ferreira, 1998), de penetração no ovócito (Gadea et al, 1998) e de fecundação (Martinez et al, 1993;Xu et al, , 1998 in vitro têm sido avaliados quanto à possibilidade de melhorar a predição da fertilidade, embora o número de estudos, na espécie suína, ainda seja pequeno.…”

Section: Boar Semen Fertility Evaluated By a Sperm-binding Assay To Aunclassified

Fertilidade de sêmen suíno avaliada pelo teste de ligação dos espermatozóides a um substrato sintético

Reis

Bernardi

Wentz

et al. 2003

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a fertilidade de sêmen suíno pelo teste de ligação de espermatozóides a um substrato sintético. A motilidade (MOT) e o porcentual de espermatozóides ligados (PEL) foram avaliados após 5, 24, 48 e 72 horas de armazenamento a 17ºC. O PEL foi determinado em soluções contendo 6,25 ou 12,5 milhões de espermatozóides/mL, com ou sem albumina sérica bovina (BSA), preparadas a partir de dois a cinco ejaculados de cada um dos quatro machos. Cinqüenta e oito leitoas foram inseminadas, uma vez, 24 horas após o início do estro. Houve correlação positiva (P = 0,0001; r = 0,33) entre a MOT e o PEL. O PEL foi maior com 12,5 milhões de espermatozóides/mL e na presença de BSA (P<0,05). Após 72 horas, o macho 3 apresentou PEL inferior ao dos outros três (P<0,05). As taxas de clivagem (TC) e de embriões morfologicamente normais não diferiram entre indivíduos, mas o macho 3 apresentou menos de 70,0% de TC no quartil superior, enquanto os outros tiveram mais de 75,0%. Os machos diferem quanto à capacidade de ligação de seus espermatozóides ao substrato sintético, a partir de 24 horas de armazenamento do sêmen. A ligação dos espermatozóides ao substrato sintético é maior com a inclusão de BSA e com o aumento da concentração espermática.Termos para indexação: albumina sérica, inseminação artificial, ligação espermatozóide-ovócito. Boar semen fertility evaluated by a sperm-binding assay to a synthetic substrateAbstract -The objective of this work was to evaluate boar semen fertility by a sperm-binding assay to a synthetic substrate. Motility (MOT) and percentage of bound sperm (PSB) were evaluated after 5, 24, 48 and 72 hours of storage at 17°C. PSB was analyzed in solutions containing 6.25 or 12.5 million of spermatozoa/mL, with or without bovine serum albumin (BSA), processed from two to five ejaculates of four boars. Fifty eight gilts were inseminated, a single time, 24 hours after the beginning of estrus. There was a positive correlation (P = 0.0001; r = 0.33) between MOT and PSB. Higher percentages of PSB were observed with 12.5 million of spermatozoa/mL and in the presence of BSA (P<0.05). After 72 hours, boar 3 showed lower PSB (P<0.05) than the other boars. The cleavage (TC) and normal embryo rates did not differ among boars, but boar 3 showed less than 70.0% of TC belonging to the superior quartile while boars 1, 2 and 4 had more than 75.0%. After 24 hours of sperm storage, boars differ in their sperm binding to the synthetic substrate. Binding of swine spermatozoa to the synthetic substrate is higher in the presence of BSA and with the increase of spermatic concentration.

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Prediction of porcine semen fertility by homologous in vitro penetration (hIVP) assay

Cited by 71 publications

References 23 publications

Pre-pubertal Di(2-ethylhexyl) Phthalate (DEHP) Exposure of Young Boars Did Not Affect SpermIn vitroPenetration Capacity of Homologous Oocytes Post-puberty

Pre-pubertal Di(2-ethylhexyl) Phthalate (DEHP) Exposure of Young Boars Did Not Affect SpermIn vitroPenetration Capacity of Homologous Oocytes Post-puberty

Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls

Fertilidade de sêmen suíno avaliada pelo teste de ligação dos espermatozóides a um substrato sintético

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