Prediction of optimum reaction conditions for the thermo‐tolerant acetylxylan esterase from <i>Neocallimastix patriciarum</i> using the response surface methodology

Pai, Cheng-Kang; Zeng, Yi‐Fang; Yueh, Pei-Ying; Chen, Ming‐Ju; Tung, Li-Chu; Liu, Je-Ruei

doi:10.1002/jctb.2322

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…Response surface methodology (RSM) was successfully used for optimizing the hot water extraction of polysaccharides from the roots of Codonopsis pilosula [9] and Prediction of optimum reaction conditions for the thermotolerant acetylxylan esterase from Neocallimastix patriciarum [10]. The advantage of RSM was that it could reduce the number of experimental trials and evaluate the interactions between multiple parameters.…”

Section: Introductionmentioning

confidence: 99%

Optimisation of Ultrasonic-Microwave-Assisted Extraction Conditions for Polysaccharides from Mulberry（Morus atropurpurea Roxb) Leaves and Evaluation of Antioxidant Activities in vitro

Liu¹

2015

Med chem

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IntroductionDuring the past decades, the polysaccharides from medicinal plants have attracted great attentions, due to their diverse and potentially significant pharmacological activities. The leaves of mulberry, as a folk medicine, has been traditionally used in Asian countries for purposes to treat fever, protect the liver, improve eyesight, strengthen joints, facilitate the discharge of urine, reduce blood glucose and lower blood pressure [1]. Mulberry leaves extracts have shown multi-direction biological activities, such as the antioxidant, antimicrobial, anti-inflammatory, anti-allergic, anti-atherosclerotic, and significant hypoglycemic effect [2][3][4][5][6][7]. A large amount of polysaccharides was contained in mulberry leaves, and their useful pharmacological effects, such as hypoglycemic and lipid-lowering activities, have been verified [8]. Polysaccharides from the leaves of mulberry (PLM) have displayed a broad application prospect in medicine and functional food.Extraction of polysaccharides is an important processing for its application or further research and development, which has prompted many research papers on the extraction technology of polysaccharides from plentiful of plants or fungus in recent years. Response surface methodology (RSM) was successfully used for optimizing the hot water extraction of polysaccharides from the roots of Codonopsis pilosula [9] and Prediction of optimum reaction conditions for the thermotolerant acetylxylan esterase from Neocallimastix patriciarum [10]. The advantage of RSM was that it could reduce the number of experimental trials and evaluate the interactions between multiple parameters. It is more effective and precise than many approaches. Ultrasonic technology and microwave technology were respectively applied to extract polysaccharides from Poria cocos [11] and cultured Cordyceps militaris [12], and the optimal extracted conditions were both obtained by RSM.However, there were few reports about optimization of ultrasonicmicrowave-assisted extraction (UMAE) of polysaccharides, especially Abstract Background: In this study, ultrasonic-microwave-assisted extraction (UMAE) was first applied to extract polysaccharides from the leaves of mulberry (PLM). The optimal conditions for polysaccharides extraction were determined by response surface methodology (RSM) along with central composite design (CCD) based on the singlefactor experiments. Extraction temperature (°C), extraction time (s), pH value, and ratio of water to raw material were investigated. Also, the antioxidant activities of PLM with UMAE were evaluated.

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Section: Introductionmentioning

confidence: 99%

Optimisation of Ultrasonic-Microwave-Assisted Extraction Conditions for Polysaccharides from Mulberry（Morus atropurpurea Roxb) Leaves and Evaluation of Antioxidant Activities in vitro

Liu¹

2015

Med chem

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Molecular cloning and characterization of a β-glucanase from Piromyces rhizinflatus

Chu

Tseng

Yueh

et al. 2011

Journal of Bioscience and Bioengineering

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The Construction of Bifunctional Fusion Xylanolytic Enzymes and the Prediction of Optimum Reaction Conditions for the Enzyme Activity

Pai¹,

Wang²,

Guo³

et al. 2012

BioResources

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Four chimeric xylanolytic enzymes were formed by fusion of a thermally stable xylanase XynCDBFV either to the N-terminus or C-terminus of a thermally stable acetylxylan esterase AxeS20E, with or without a Gly-rich flexible linker (S2). The three-dimensional (3D) structures of the chimeric enzymes were predicted using the I-TASSER server, and the results indicated that the structures of Axe-S2-Xyn and Xyn-S2-Axe were more similar to the native structures than were those of Axe-Xyn and Xyn-Axe. Axe-S2-Xyn and Xyn-S2-Axe were expressed in Escherichia coli and purified by means of affinity chromatography. Response surface modeling (RSM), combined with central composite design (CCD) and regression analysis, was then employed to optimize the xylanase activities of the chimeric enzymes. Under the optimal conditions, Xyn-S2-Axe had greater hydrolytic activities on natural xylans and rice straw than did the parental enzymes. These results suggested that the chimeric enzyme Xyn-S2-Axe could be effective at hydrolyzing xylan in biomass and that it has potential to be used in a range of biotechnological applications.

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Prediction of optimum reaction conditions for the thermo‐tolerant acetylxylan esterase from Neocallimastix patriciarum using the response surface methodology

Cited by 3 publications

References 38 publications

Optimisation of Ultrasonic-Microwave-Assisted Extraction Conditions for Polysaccharides from Mulberry（Morus atropurpurea Roxb) Leaves and Evaluation of Antioxidant Activities in vitro

Optimisation of Ultrasonic-Microwave-Assisted Extraction Conditions for Polysaccharides from Mulberry（Morus atropurpurea Roxb) Leaves and Evaluation of Antioxidant Activities in vitro

Molecular cloning and characterization of a β-glucanase from Piromyces rhizinflatus

The Construction of Bifunctional Fusion Xylanolytic Enzymes and the Prediction of Optimum Reaction Conditions for the Enzyme Activity

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