Prediction of non-alcoholic fatty-liver disease and liver fat content by serum molecular lipids

Orešič, Matej; Hyötyläinen, Tuulia; Kotronen, Anna; Gopalacharyulu, Peddinti; Nygrén, Heli; Arola, Johanna; Castillo, Sandra; Mattila, Ismo; Hakkarainen, Antti; Borra, Ronald; Honka, Miikka-Juhani; Verrijken, An; Francque, Sven; Iozzo, Patricia; Leivonen, Marja; Jaser, Nabil; Juuti, Anne; Sørensen, Thorkild I A; Nuutila, Pirjo; Gaal, Luc Van; Yki‐Järvinen, Hannele

doi:10.1007/s00125-013-2981-2

Cited by 133 publications

(81 citation statements)

References 47 publications

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“…Hierarchical cluster analysis also shows the good clustering between the ND and HFD groups with a significant negative association of PUFA TAGs with obesity. These results were consistent with several other models, as for examples SFA containing TAGs were decreased in diet induced weight loss model 29 and increased in serum of individuals with nonalcoholic fatty-liver disease (NAFLD) 33 . In contrast, PUFA containing TAGs were decreased by 40% in ob/ob mice 34 and negatively associated with NAFLD 33 .…”

Section: Discussionsupporting

confidence: 91%

Untargeted Lipidomic Analysis of Plasma from High-fat Diet-induced Obese Rats Using UHPLC–Linear Trap Quadrupole–Orbitrap MS

Gowda

Chen

et al. 2020

Anal. Sci.

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High fat diet (HFD)-induced obesity is a primary risk factor for serious health problems. Although much research has been performed at the genomic level, lipidomic studies were limited. In this study, we aim to obtain the comprehensive profile of circulating plasma lipids, which are altered in rodent rat obesity by untargeted liquid chromatography-mass spectrometry. Rats were fed with HFD for 8 weeks have the increased body weight, liver and adipose tissue weight. The analysis results revealed that polyunsaturated fatty acids (PUFAs) and their corresponding phosphatidylcholine, phosphatidylinositol, and phosphatidylserine were significantly decreased in rats fed with HFD. In contrast, less unsaturated and ether type phosphatidylglycerols were increased. The triacylglycerides (TAGs) having saturated FA were increased in HFD condition, whereas TAGs having PUFA were decreased. The levels of many plasma lipids were altered, interestingly PUFAs derived lipids were negatively associated with obesity signifies the importance of PUFAs enriched diet to overwhelm obesity associated diseases.

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Section: Discussionsupporting

confidence: 91%

Untargeted Lipidomic Analysis of Plasma from High-fat Diet-induced Obese Rats Using UHPLC–Linear Trap Quadrupole–Orbitrap MS

Gowda

Chen

et al. 2020

Anal. Sci.

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“…Orešič et al [76] focused on UHPLC-MS analysis of lipids in blood samples of 679 well-characterized individuals in whom liver-fat content was measured using proton magnetic resonance spectroscopy or liver biopsy. Individuals with non-alcoholic fatty-liver disease (NAFLD) had increased TGs with low carbon number and double-bond content while LPCs and ether PLs were diminished in those with NAFLD.…”

Section: Highlights Of Recent Lipidomics Studiesmentioning

confidence: 99%

Comprehensive analysis of lipids in biological systems by liquid chromatography-mass spectrometry

Čajka

Fiehn

2014

TrAC Trends in Analytical Chemistry

491

417

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Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics has been a subject of dramatic developments over the past decade. This review focuses on state of the art in LC-MS-based lipidomics, covering all the steps of global lipidomic profiling. On the basis of review of 185 original papers and application notes, we can conclude that typical LC-MS-based lipidomics methods involve: (1) extraction using chloroform/MeOH or MTBE/MeOH protocols, both with addition of internal standards covering each lipid class; (2) separation of lipids using short microbore columns with sub-2-μm or 2.6–2.8-μm (fused-core) particle size with C18 or C8 sorbent with analysis time <30 min; (3) electrospray ionization in positive- and negative-ion modes with full spectra acquisition using high-resolution MS with capability to MS/MS. Phospholipids (phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, phosphatidylserines, phosphatidylglycerols) followed by sphingomyelins, di- and tri-acylglycerols, and ceramides were the most frequently targeted lipid species.

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“…The increase of TGs with low carbon number and double bond count does appear to be specific to CD progression. These TGs are associated, in adults, with elevated liver fat in non-alcoholic fatty liver disease (NAFLD) 58,59 , reflecting increased de novo lipogenesis and adipose tissue lipolysis. In fact, CD patients have, interestingly, been found to be at increased risk of NAFLD 60 .…”

Section: Discussionmentioning

confidence: 99%

Persistent alterations in plasma lipid profiles prior to introduction of gluten in the diet associate with progression to celiac disease

Sen

Carlsson

Virtanen

et al. 2018

Preprint

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Background and AimsCeliac disease (CD) is a chronic enteropathy characterized by an autoimmune reaction in the small intestine in genetically-susceptible individuals. Gluten is the required environmental trigger of clinical CD, but the underlying causes of the autoimmune reaction remain unknown. Herein, we apply lipidomics to elucidate the early events preceding clinical CD in a prospective study of children observed from birth until diagnosis of CD and subsequent introduction of a gluten-free diet. MethodsMass spectrometry-based lipidomics profiling was applied to a longitudinal series of 233 plasma samples from the Type 1 Diabetes Prediction and Prevention (DIPP) study, spanning the period between birth and the introduction of a gluten-free diet following CD diagnosis (n=23 CD progressors, n=23 controls matched for gender, HLA risk, period of birth, and age). Results23 children progressed to CD at a mean age of 4.8 years. They showed increased amounts of triacylglycerols (TGs) of low carbon number and double bond count and a decreased level of phosphatidylcholines by 3 months of age as compared to controls. These differences were exacerbated with age but were not observed at birth. No significant differences were observed in essential (dietary) TGs such as those containing polyunsaturated fatty acids. ConclusionOur findings suggest that abnormal lipid metabolism associated with development of clinical CD may occur prior to the introduction of gluten to the diet. Moreover, our data suggest that the specific TGs found elevated in CD progressors may be due to a host response to compromised intake of essential lipids in the small intestine, requiring de novo lipogenesis.

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Prediction of non-alcoholic fatty-liver disease and liver fat content by serum molecular lipids

Cited by 133 publications

References 47 publications

Untargeted Lipidomic Analysis of Plasma from High-fat Diet-induced Obese Rats Using UHPLC–Linear Trap Quadrupole–Orbitrap MS

Untargeted Lipidomic Analysis of Plasma from High-fat Diet-induced Obese Rats Using UHPLC–Linear Trap Quadrupole–Orbitrap MS

Comprehensive analysis of lipids in biological systems by liquid chromatography-mass spectrometry

Persistent alterations in plasma lipid profiles prior to introduction of gluten in the diet associate with progression to celiac disease

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