Prediction of new likely emergent amino acid point mutations from USA, UK, Italy, France, Brazil, and India SARS-CoV-2 variants: a possible source for more reliable cocktail coronavirus vaccine

Abstract

Objectives The interaction between genome components of the vaccines, host cells and SARS-CoV-2 variants can cause mutation of amino acids at high random frequency. Hence the present study is aimed at using the codon bases –amino acids components of some available strains with a view to determing new likely emergent strains of SARS-CoV-2. Methods Some genome sizes and lengths of SARS-CoV-2 variants were either searched from literatures calculated. Point mutation of a single amino acid was deduced from 2/3 of sets of codon bases responsible for expression of amino acids. One base pair of 0.0047nm Codon Base Table was used to deduce the likely missense amino acids at probability of one-twentieth. Keyfindings New generated codon bases gave rise to new emergent strains of varying number of amino acid pairs. Amino acids have reappeared and disappeared in some strains. Nine strains altogether have shown stop codon bases and the remaining strains have tendency to form stop codons. Adenine has highest frequency of the stop codon bases whereas cytosine is not among stop codon. A total of 906 new variants were deduced from 54 coronavirus strains which initially lacked stop codons. The newly predicted strains may become less pathogenic and serve as immunogen via glycosylation. Strains with higher number of codon bases undergo mutation faster than that may end up in stop codons. Conclusion Hence the likely emergent strains could be less virulent, less pathogenic and many from glycans that could serve as source for manufacturing of more reliable coronavirus vaccines.