Prediction of miRNA interaction with mRNA of stroke candidate genes

Kondybayeva, Aida; Akimniyazova, Aigul; Kamenova, Saltanat; Duchshanova, Gulsum; Aisina, Dana; Goncharova, Alla V.; Иващенко, А. Т.

doi:10.1007/s10072-019-04158-x

Cited by 14 publications

(17 citation statements)

References 29 publications

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“…In this study, we attempted to explore the association between circPHKA2 and SOD2 in cerebral IS, and its cell model was established in human brain microvascular endothelial cells (HBMEC) induced by oxygen-glucose deprivation (OGD). Eventually, a circPHKA2-microRNA (miR-574-5p)-SOD2 axis was highlighted, and miR-574-5p is recommended as one of the markers for stroke diagnosis by interacting with multiple messenger RNAs (mRNAs) of stroke candidate genes [17].…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circPHKA2 Relieves OGD‐Induced Human Brain Microvascular Endothelial Cell Injuries through Competitively Binding miR‐574‐5p to Modulate SOD2

Yang

Zhong

et al. 2021

Oxidative Medicine and Cellular Longevity

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Background. Circular RNA phosphorylase kinase regulatory subunit alpha 2 (circPHKA2; hsa_circ_0090002) has a significantly, specifically different expression in acute ischemic stroke (AIS) patients’ blood. Here, we intended to investigate the role and mechanism of circPHKA2 in oxygen-glucose deprivation- (OGD-) induced stoke model in human brain microvascular endothelial cells (HBMEC). Methods. Expression of circPHKA2, microRNA- (miR-) 574-5p, and superoxide dismutase-2 (SOD2) was detected by quantitative PCR and western blotting. Cell injury was measured by detecting cell proliferation (EdU assay and CCK-8 assay), migration (transwell assay), neovascularization (tube formation assay), apoptosis (flow cytometry and western blotting), endoplasmic reticulum stress (western blotting), and oxidative stress (assay kits). Direct intermolecular interaction was determined by bioinformatics algorithms, dual-luciferase reporter assay, biotin-labelled miRNA capture, and argonaute 2 RNA immunoprecipitation. Results. circPHKA2 was downregulated in AIS patients’ blood in SOD2-correlated manner. Reexpressing circPHKA2 rescued EdU incorporation, cell viability and migration, tube formation, B cell lymphoma-2 (Bcl-2) expression, and SOD activity of OGD-induced HBMEC and alleviate apoptotic rate and levels of Bcl-2-associated protein (Bax), glucose-regulated protein 78 kD (GRP78), C/EBP-homologous protein (CHOP), caspase-12, reactive oxygen species (ROS), and malondialdehyde (MDA). Additionally, blocking SOD2 partially attenuated these roles of circPHKA2 overexpression. Molecularly, circPHKA2 upregulated SOD2 expression via interacting with miR-574-5p, and miR-574-5p could target SOD2. Similarly, allied to neurovascular protection of circPHKA2 was the downregulation of miR-574-5p. Conclusion. circPHKA2 could protect HBMEC against OGD-induced cerebral stroke model via the miR-574-5p/SOD2 axis, suggesting circPHKA2 as a novel and promising candidate in ischemic brain injury.

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Section: Introductionmentioning

confidence: 99%

Circular RNA circPHKA2 Relieves OGD‐Induced Human Brain Microvascular Endothelial Cell Injuries through Competitively Binding miR‐574‐5p to Modulate SOD2

Yang

Zhong

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…In addition, miRNAs are considered novel non-invasive biomarkers of the instability of atherosclerotic plaques and have been associated ischemic disorders (Churov et al, 2019). Their detection in the blood of patients may be a promising direction for the diagnosis of atherosclerosis complications such as ischemic stroke and myocardial infarction (Kanuri et al, 2018;Desjarlais et al, 2019;Moghaddam et al, 2019;Vargas-Alarcon et al, 2019;Velle-Forbord et al, 2019;Wiese et al, 2019;Kondybayeva et al, 2020). When the pathogenic role of a specific miRNA is confirmed, it can be considered a potential therapeutic target (Friedman et al, 2009;Fang et al, 2013;Moghaddam et al, 2019;Kazemi et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

In silico Prediction of miRNA Interactions With Candidate Atherosclerosis Gene mRNAs

et al. 2020

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The involvement of genes and miRNAs in the development of atherosclerosis is a challenging problem discussed in recent publications. It is necessary to establish which miRNAs affect the expression of candidate genes. We used known candidate atherosclerosis genes to predict associations. The quantitative characteristics of interactions of miRNAs with mRNA candidate genes were determined using the program, which identifies the localization of miRNA binding sites in mRNA, the free energy interaction of miRNA with mRNA. In mRNAs of GAS6 and NFE2L2 candidate genes, binding sites of 21 miRNAs and of 15 miRNAs, respectively, were identified. In IRS2 mRNA binding sites of 25 miRNAs were located in a cluster of 41 nt. In ADRB3, CD36, FASLG, FLT1, PLA2G7 , and PPARGC1A mRNAs, clusters of miR-466, ID00436.3p-miR, and ID01030.3p-miR BS were identified. The organization of overlapping miRNA binding sites in clusters led to their compaction and caused competition among the miRNAs. The binding of 53 miRNAs to the mRNAs of 14 candidate genes with free energy interactions greater than −130 kJ/mole was determined. The miR-619-5p was fully complementary to ADAM17 and CD36 mRNAs, ID01593.5p-miR to ANGPTL4 mRNA, ID01935.5p-miR to NFE2L2 , and miR-5096 to IL18 mRNA. Associations of miRNAs and candidate atherosclerosis genes are proposed for the early diagnosis of this disease.

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“…In CSCC, we showed that hsa-mir-145 overexpression had a better overall survival. hsa-mir-1250, hsa-mir-1273d, hsa-mir-2277, and hsa-mir-299 play roles in many diseases (31)(32)(33).…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel circRNA, hsa_circ_0065898, that regulates tumor growth in cervical squamous cell carcinoma

Ni¹,

Liu²,

Deng³

2021

Transl Cancer Res TCR

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Background: Circular RNAs (circRNAs) were reported to play an important role in regulating tumor pathogenesis. The molecular mechanism of circRNAs in cervical squamous cell carcinoma (CSCC) remains poorly understood. We aimed to identify the circRNAs differentially expressed, and to investigate the role of a novel circRNA, hsa_circ_0065898, in regulating proliferation, migration, and invasion in CSCC.Methods: The online Kaplan-Meier Plotter was used to analyze the relationship between miRNA expression and overall survival. Bioinformatics tools, such as R, Cytoscape, and Perl, were used to analyze the Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, proteinprotein interaction (PPI) network, and regulatory network. The expression level of hsa_circ_0065898 in CSCC cell lines was evaluated using quantitative polymerase chain reaction in vitro. The cell counting kit-8 (CCK-8) and transwell assays were used to assess cell proliferation, migration, and invasion.Results: circRNA expression data (GSE102686) was downloaded from the Gene Expression Omnibus database, and this included data from 5 CSCC patients and 5 normal tissues. 13 differentially expressed circRNAs were identified, which included 9 upregulated circRNAs and 4 downregulated circRNAs. GO enrichment analysis showed that the target genes of miRNAs associated with hsa_circ_0065898 were enriched in ubiquitin-protein transferase activity, ubiquitin-like protein transferase activity, core promoter sequence-specific DNA binding, mRNA 3'-UTR AU-rich region binding, core promoter binding, and so on. KEGG showed that the Hippo and p53 signaling pathways played significant role in the pathway network.Hsa_circ_0065898 was significantly overexpressed in the CSCC cell lines. Hsa_circ_0065898 facilitated cell proliferation, migration, and invasion in CSCC.Conclusions: This study identified differentially expressed circRNAs and constructed the regulatory network of hsa_circ_0065898 targeting microRNAs and mRNAs. We demonstrated that hsa_circ_0065898 promoted CSCC cell proliferation, migration, and invasion. Hence, hsa_circ_0065898 might be useful as a biomarker for CSCC diagnosis and targeted therapy.

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Prediction of miRNA interaction with mRNA of stroke candidate genes

Cited by 14 publications

References 29 publications

Circular RNA circPHKA2 Relieves OGD‐Induced Human Brain Microvascular Endothelial Cell Injuries through Competitively Binding miR‐574‐5p to Modulate SOD2

Circular RNA circPHKA2 Relieves OGD‐Induced Human Brain Microvascular Endothelial Cell Injuries through Competitively Binding miR‐574‐5p to Modulate SOD2

In silico Prediction of miRNA Interactions With Candidate Atherosclerosis Gene mRNAs

Identification of a novel circRNA, hsa_circ_0065898, that regulates tumor growth in cervical squamous cell carcinoma

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