Prediction of K562 Cells Functional Inhibitors Based on Machine Learning Approaches

Abstract

Background: β thalassemia is a common monogenic genetic disease that is very harmful to human health. The disease arises is due to the deletion of or defects in β-globin, which reduces synthesis of the β-globin chain, resulting in a relatively excess number of α-chains. The formation of inclusion bodies deposited on the cell membrane causes a decrease in the ability of red blood cells to deform and a group of hereditary haemolytic diseases caused by massive destruction in the spleen. Methods: In this work, machine learning algorithms were employed to build a prediction model for inhibitors against K562 based on 117 inhibitors and 190 non-inhibitors. Results: The overall accuracy (ACC) of a 10-fold cross-validation test and an independent set test using Adaboost were 83.1% and 78.0%, respectively, surpassing Bayes Net, Random Forest, Random Tree, C4.5, SVM, KNN and Bagging. Conclusion: This study indicated that Adaboost could be applied to build a learning model in the prediction of inhibitors against K526 cells.

Production and Characterization of K562 Cellular Clones Hyper-Expressing the Gene Encoding α-Globin: Preliminary Analysis of Biomarkers Associated with Autophagy

“…In this context, the availability of an experimental model system that mimics the excess in α-globin chain production and that is prone to autophagy and apoptosis could be of great interest in the development of protocols aimed at reducing free α-globin chains and activating autophagy. Unfortunately, one of the most commonly used cellular systems, the erythroleukemia K562 cell line [ 18 , 19 , 20 ], exhibits an α-thalassemic-like phenotype characterized by a low transcription of α-globin genes and an absent production of α-globin protein [ 21 ]. The K562 cell line was selected for this study as well characterized with respect to its response to several fetal hemoglobin inducers, including rapamycin (sirolimus), which was recently proposed as a potent modulator of autophagy in erythroid cells, with an associated decrease in free α-globin chains [ 13 ].…”