Prediction of ingredient quality and the effect of a combination of xylanase, amylase, protease and phytase in the diets of broiler chicks. 2. Energy and nutrient utilisation

Abstract

1. In order to investigate the effects of xylanase, amylase, protease and phytase in the diets of broiler chickens containing graded concentrations of metabolisable energy (ME), two 42-d experiments were conducted using a total of 2208 broiler chicks (8 treatments with 12 replicate pens in each experiment). 2. Four diets including one positive and three negative control diets were used. Three maize/soybean meal-based negative control (NC) diets were formulated to be identical in available phosphorus (P), calcium (Ca) and amino acids but NC1 contained approximately 0.17 MJ/kg less ME than NC2 and approximately 0.34 MJ/kg less ME than NC3. A positive control (PC) was fed for comparison and was formulated to be adequate in all nutrients, providing approximately 0.63 MJ/kg ME, 0.13% available P, 0.12% Ca and 1 to 2% amino acids more than NC1. 3. The reduction in nutrient density between NC1 and PC was determined using ingredient quality models Avichecktrade mark Corn and Phychecktrade mark that can predict the response to exogenous enzymes in maize/soybean meal-based broiler diets. Supplementation of each diet with or without a cocktail of xylanase, amylase, protease and phytase gave a total of 8 dietary treatments in a 4 x 2 factorial arrangement. The same treatments and diet designs were used in both experiments but conducted in different locations using different batches of maize, soybean meal and minor ingredients. 4. In both experiments, digestibility was improved by the addition of exogenous enzymes, particularly those for P, Ca and certain amino acids. In addition, the supplementation of the PC with enzymes elicited a positive response indicating that over-the-top addition of xylanase, amylase, protease and phytase may offer a nutritionally and economically viable alternative to feed cost reduction. 5. It can be concluded that the digestibility of nutrients by broilers fed on maize/soybean meal-based diets can be improved by the use of a combination of xylanase, amylase, protease and phytase.

Effect of feed form, pellet diameter and enzymes supplementation on growth performance and nutrient digestibility of broiler during days 21-37 of age

“…The greater effect of both enzymes when added together indicated a synergetic effect and this is in agreement with the results of Cowieson et al (2006) and Yang et al (2010). The use of an enzyme complex containing carbohydrases and phytase can improve the utilization of phytate P, energy, protein, and Ca concentrations.…”

Effect of phytase with or without multienzyme supplementation on performance and nutrient digestibility of young broiler chicks fed mash or crumble diets

“…The use of an enzyme complex containing carbohydrases and phytase was suggested as a tool to decrease dietary concentration of nutrient, i.e. avP, AME, CP/amino acids, and Ca in poultry feeds due to improved nutrient utilisation (Attia, 2003;Attia et al, 2001;Cowieson et al, 2006;Yang et al, 2010). However, the lack of a significant interaction effect on performance and nutrient digestibility for the whole study period indicated that either multienzyme plus phytase or phytase alone may be adequate, that supplementation to mash or crumble feeds is essential, and that the influence of enzymes is independent of type of feed.…”

“…However, improved performance of poultry depends on dietary composition and type of supplemented enzyme (Attia, 2003;Abudabos, 2012;Nourmohammadi et al, 2012). The use of an enzyme complex containing carbohydrases and phytase should improve the ability of broilers, laying hens, ducks and Japanese quail to use the energy, protein, P and Ca they obtain from the diet (Attia, 2003;Attia et al, 2003aAttia et al, , 2003bAttia et al, , 2008Cowieson et al, 2006;Yang et al, 2010) and a small amount of these nutrients can be added to the diet. This study aimed to evaluate the effect of phytase with or without multienzyme supplementation on performance and nutrient digestibility of young broiler chicks fed mash or crumble diets.…”

Enzyme complex containing carbohydrases and phytase improves growth performance and bone mineralization of broilers fed reduced nutrient corn-soybean-based diets

“…However, the assessment of the nutrient specifications for the enzyme or the reduction in dietary nutrient concentration for a multi-enzyme complex, targeted for different substrates, with different modes of action, but sharing effects on performance and nutrient digestibility could be much more difficult to predict. Many factors can influence the response to an enzyme complex because it depends on the enzyme specificity, concentration of the substrate, doses of enzymes, interactions between enzymes, ingredient quality and type, level of nutrients in the diet, and age of animals (Bedford, 2002;Rosen, 2002;Cowieson and Adeola, 2005;Cowieson et al, 2006c).…”