Prediction of in-vitro developmental competence of early cleavage-stage mouse embryos with compact time-lapse equipment

Pribenszky, Csaba; Losonczi, Eszter; Molnár, Miklós; Láng, Zsolt; Mátyás, Szabolcs; Rajczy, Klára; Molnár, Katalin; Kovács, Péter; Nagy, Péter; Conceicao, Jason; Vajta, Gábor

doi:10.1016/j.rbmo.2009.12.007

Cited by 81 publications

(63 citation statements)

References 45 publications

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“…The closer the curve is to the upper left corner (sensitivityZ1 and specificityZ1), the higher the accuracy of the model (larger area under the curve). NT, non-treated; PsA, psammaplin A; VitC, vitamin C. embryos were similar, or even higher, than those previously obtained in our laboratory for non-imaged ICSI and SCNT embryos cultured in the same incubator (Mallol et al 2015), confirming that embryo development was not compromised by the use of the PrimoVision imaging system, as already reported by Pribenszky et al (2010) for IVF mouse embryos, which are not as sensitive as SCNT embryos to culture conditions (Gao et al 2003).…”

Section: Discussionsupporting

confidence: 83%

“…The main purpose of the present work was to obtain a detailed description of the morphokinetics of ICSI and SCNT B6CBAF1 mouse embryos, from the one-cell to the blastocyst stages, using a commercially available time-lapse monitoring system which has been shown not to compromise the development of fertilized mouse embryos (Pribenszky et al 2010). Furthermore, we aimed to analyze the effect of PsA and VitC treatments on the morphokinetics of SCNT embryos.…”

Section: Introductionmentioning

confidence: 99%

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Morphokinetics of cloned mouse embryos treated with epigenetic drugs and blastocyst prediction

et al. 2016

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Time-lapse monitoring of somatic cell nuclear transfer (SCNT) embryos may help to predict developmental success and increase birth and embryonic stem cells (ESC) derivation rates. Here, the development of ICSI fertilized embryos and of SCNT embryos, non-treated or treated with either psammaplin A (PsA) or vitamin C (VitC), was monitored, and the ESC derivation rates from the resulting blastocysts were determined. Blastocyst rates were similar among PsA-treated and VitC-treated SCNT embryos and ICSI embryos, but lower for nontreated SCNT embryos. ESC derivation rates were higher in treated SCNT embryos than in non-treated or ICSI embryos. Time-lapse microscopy analysis showed that non-treated SCNT embryos had a delayed development from the second division until compaction, lower number of blastomeres at compaction and longer compaction and cavitation durations compared with ICSI ones. Treatment of SCNT embryos with PsA further increased this delay whereas treatment with VitC slightly reduced it, suggesting that both treatments act through different mechanisms, not necessarily related to their epigenetic effects. Despite these differences, the time of completion of the third division, alone or combined with the duration of compaction and/or the presence of fragmentation, had a strong predictive value for blastocyst formation in all groups. In contrast, we failed to predict ESC derivation success from embryo morphokinetics. Time-lapse technology allows the selection of SCNT embryos with higher developmental potential and could help to increase cloning outcomes. Nonetheless, further studies are needed to find reliable markers for full-term development and ESC derivation success.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Morphokinetics of cloned mouse embryos treated with epigenetic drugs and blastocyst prediction

et al. 2016

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“…However, since images are automatically recorded, the embryo assessment can be completed whenever the laboratory technician finds it convenient, allowing for a more flexible evaluation. Furthermore, recent studies [2,18,23,32,41] have addressed the potential role of time-lapse monitoring in selection of competent embryos. Apart from a more detailed and flexible embryo evaluation, these studies suggest that time-lapse monitoring may provide new dynamic markers of embryo competence.…”

Section: Discussionmentioning

confidence: 99%

“…Time-lapse monitoring overcomes this limitation without exposing the embryos to environmental changes. Moreover, several recent studies suggest that timelapse monitoring may introduce new dynamic markers of embryonic competence ( [2,18,23,32,41]). …”

Section: Introductionmentioning

confidence: 99%

“…Before introducing timelapse monitoring in a clinical setting, the safety of the instrument must therefore be properly documented. Comparisons have been made between embryos, both mouse and human, cultured in conventional incubators and time-lapse instruments, without reports of any adverse effects on development or implantation rate [5,15,18,24,26,32,41]. However, none of the previous studies were conducted as randomized clinical trials and the included human embryos derived from oocyte donor cycles [5] may not be representative of embryos from infertile couples.…”

Section: Introductionmentioning

confidence: 99%

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A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

Kirkegaard

Hindkjær

Grøndahl

et al. 2012

J Assist Reprod Genet

129

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Purpose Time-lapse monitoring allows for a flexible embryo evaluation and potentially provides new dynamic markers of embryo competence. Before introducing timelapse monitoring in a clinical setting, the safety of the instrument must be properly documented. Accordingly, the aim of this study was to evaluate the safety of a commercially available time-lapse incubator. Methods In a two center, randomized, controlled, clinical trial 676 oocytes from 59 patients in their 2nd or third treatment cycle, age <38 years and ≥8 oocytes retrieved were cultured in the time-lapse incubator or in a conventional incubator. The primary outcome was proportion of 4-cell embryos on day 2. Secondary outcomes were proportion of 7-8 cell embryos on day 3 and proportion of blastocysts on day 5. Implantation pregnancy rates were registered based on presence of fetal heart activity visualized by ultrasound 8 weeks after embryo transfer. Results No significant difference was found between the time-lapse incubator (TLI) and conventional incubator (COI) in proportion of 4-cell embryos on day 2 irrespective of whether data was analyzed according to ITT (RR TLI/COI : 0.81 (0.65; 1.02)) or PP (RR TLI/COI : 0.80 (0.63; 1.01)). Nor were any significant differences detected in the secondary endpoints; i.e. proportion of 7-8-cell embryos on day three ITT (RR TLI/COI : 0.96 (0.73; 1.26)); PP (RR TLI/COI : 0.95 (0.72; 1.26)) and proportion of blastocysts on day five ITT (RR TLI/COI : 1.09 (0.84; 1.41)); PP (RR TLI/COI : 1.09 (0.83: 1.41)). We found no differences in clinical pregnancy rate or implantation rate. Conclusion Culture in the time-lapse incubator supports embryonic development equally to a conventional incubator.

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New Horizons/Developments in Time‐Lapse Morphokinetic Analysis of Mammalian Embryos

Serdaroğullari¹,

Fındıklı²,

Bahçeci³

2016

Human Reproduction

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Prediction of in-vitro developmental competence of early cleavage-stage mouse embryos with compact time-lapse equipment

Cited by 81 publications

References 45 publications

Morphokinetics of cloned mouse embryos treated with epigenetic drugs and blastocyst prediction

Morphokinetics of cloned mouse embryos treated with epigenetic drugs and blastocyst prediction

A randomized clinical trial comparing embryo culture in a conventional incubator with a time-lapse incubator

New Horizons/Developments in Time‐Lapse Morphokinetic Analysis of Mammalian Embryos

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