Prediction of future metastasis and molecular characterization of head and neck squamous-cell carcinoma based on transcriptome and genome analysis by microarrays

Rickman, David S.; Millon, Régine; Reyniès, Aurélien de; Thomas, Emilie; Wasylyk, Christine; Muller, Danièle; Abecassis, Joseph; Wasylyk, Bohdan

doi:10.1038/onc.2008.251

Cited by 140 publications

(128 citation statements)

References 42 publications

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“…Mining of existing arrays is a much less labor-intensive process than real-time PCR for individual microRNAs, but only limited arrays can be mined, as not all microarrays have the relevant probe. Encouragingly, the Affymetrix probes to the relevant gene for hsa-miR-210 were both prognostic in the series of Rickman et al, 30 and were particularly predictive of long-term outcome (>50 months). The probes were not significant in our series, but there were some differences between the series, particularly in terms of site (the Rickman series having more hypopharynx tumors) and stage at diagnosis (our series containing more stage IV cases).…”

Section: Discussionmentioning

confidence: 92%

“…Expression values for the genes containing the microRNA precursors and hypoxia-related genes were determined from Affymetrix GeneChips in the published datasets of Winter et al 7 and Rickman et al 30 Data were preprocessed and normalized as previously described. 7 A published 99-gene hypoxia up-regulated metagene was used that has been derived in HNSCC from genes clustering with known hypoxia-regulated genes.…”

Section: Microarray Datamentioning

confidence: 99%

“…Specifically, a lower level of pre-miR-210 was associated with an improved prognosis, but this was not statistically significant for a single probe in our series. Next, we examined a series of patients with HNSCC for which Affymetrix gene expression data and clinical follow-up were publically available; patient demographics and details may be found in Rickman et al 30 In the 81 patients studied, tumor expression of both 236480_at and 230710_at were adverse prognostic factors for overall survival in univariate analysis when considered as binary variables divided by median value (236480_at, P ¼ .009; 230710_at, P ¼ .033) (Fig. 5).…”

Section: Microrna Association With Recurrence-free Survival and Overamentioning

confidence: 99%

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hsa‐miR‐210 is a marker of tumor hypoxia and a prognostic factor in head and neck cancer

Gee

Camps

Buffa

et al. 2010

Cancer

224

157

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BACKGROUND:Hypoxia is an important mechanism of treatment resistance in head and neck squamous cell carcinoma (HNSCC). MicroRNAs are short noncoding RNAs that regulate multiple mRNAs and are frequently dysregulated in cancer. The authors have investigated the role of 3 microRNAs, including the hypoxia-induced hsa-miR-210, as potential markers of hypoxia or prognosis. METHODS: Three hypoxia-related microRNAs, hsa-miR-210, hsa-miR-21, and hsa-miR-10b, were measured in 46 samples from patients with HNSCC. Expression levels were correlated with clinicopathological variables and other markers of hypoxia: a published 99-gene hypoxia metagene, individual hypoxia-related genes such as TWIST1, and immunohistochemical expression of hypoxia-inducible factor 1 and its target gene carbonic anhydrase 9. We then performed survival analyses to investigate the prognostic significance of these microRNAs. RESULTS: Only the level of hsa-miR-210 was significantly correlated with other markers of hypoxia, including the 99-gene hypoxia metagene (rho ¼ 0.67, P < .001). We found no association between hsa-miR-210, hsamiR-21, or hsa-miR-10b and clinicopathological variables such as tumor size, differentiation, and stage. However, high levels of hsa-miR-210 were associated with locoregional disease recurrence (P ¼ .001) and short overall survival (P ¼ .008). hsa-miR-21 and hsa-miR-10b had no prognostic significance. CONCLUSIONS: Expression of hsa-miR-210 in head and neck cancer correlates with other approaches for assessing hypoxia and is associated with prognosis. This warrants further study as a classification marker of patients for therapies involving modulation of hypoxia.

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Section: Discussionmentioning

confidence: 92%

Section: Microarray Datamentioning

confidence: 99%

Section: Microrna Association With Recurrence-free Survival and Overamentioning

confidence: 99%

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hsa‐miR‐210 is a marker of tumor hypoxia and a prognostic factor in head and neck cancer

Gee

Camps

Buffa

et al. 2010

Cancer

224

157

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“…19,20 Oncomine Ò Power Tools 21 was used to analyze the relationship between CD24 gene expression and oral cancer progression as well as patient outcome based on publically available datasets. 22,23 We found that oral cavity squamous cell carcinoma cancer patients with regional lymph node metastasis showed lower expression of CD24 mRNA compared to patients without metastasis (Fig. 1A).…”

Section: Low Cd24 Expression In Oral Cancer Is Associated With Poor Pmentioning

confidence: 96%

CD24 blunts oral squamous cancer development and dampens the functional expansion of myeloid-derived suppressor cells

et al. 2016

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CD24 expression has been implicated in the oncogenesis of multiple types of cancer and high tumor expression is considered a poor prognosis factor; however, the role of CD24 in oral cancer progression is unknown. Unlike other cancer types, we found that higher CD24 levels in human oral cancers are correlated to lower clinical stage and better overall survival. We then dissected the role of CD24 and mechanisms in oral cancer pathogenesis in mice using a genetic strategy and demonstrated that CD24 deficiency increased the oral cavity tumor burden in response to the carcinogen 4-nitroquioline 1-oxide (4-NQO). Immune profile analysis showed a significant expansion as well as increased suppressive function of myeloid-derived suppressor cells (MDSCs) in CD24 ¡/¡ mice, but no apparent impairment in T cells, B cells, or dendritic cells. Further, studies with an orthotopically transplanted syngeneic squamous carcinoma model in the tongue of CD24 ¡/¡ and CD24 C/¡ mice confirmed the protective roles of CD24 against cancer. Moreover, the difference in tumor growth between CD24 ¡/¡ and CD24 C/¡ mice was blunted by immunodepletion of MDSCs. We conclude that CD24 expression impedes MDSC expansion and function, and thus slows oral cancer oncogenesis. This study is the first to examine the role of CD24 in a de novo oral cancer model, and it highlights the need to consider the immune regulatory roles of CD24 in the development of CD24-targeted therapy for cancer.

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“…An extra dataset concerning breast carcinoma 46 was used for studying expression variability and treatment response. We also used two datasets for ovarian (TCGA consortium) 47 and head and neck carcinoma 48,49 each. The total number of tumors analyzed in this study amounts to 5953 (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Impact of chemotactic factors and receptors on the cancer immune infiltrate: a bioinformatics study revealing homogeneity and heterogeneity among patient cohorts

et al. 2018

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Multiple soluble factors including proteins (in particular chemokines), non-proteinaceous factors released by dead cells, as well as receptors for such factors (in particular chemokine receptors, formyl peptide receptors and purinergic receptors), influence the recruitment of distinct cell subsets into the tumor microenvironment. We performed an extensive bioinformatic analysis on tumor specimens from 5953 cancer patients to correlate the mRNA expression levels of chemotactic factors/receptors with the density of immune cell types infiltrating the malignant lesions. This meta-analysis, which included specimens from breast, colorectal, lung, ovary and head and neck carcinomas as well as melanomas, revealed that a subset of chemotactic factors/receptors exhibited a positive and reproducible correlation with several infiltrating cell types across various solid cancers, revealing a universal pattern of association. Hence, this meta-analysis distinguishes between homogeneous associations that occur across different cancer types and heterogeneous correlations, that are specific of one organ. Importantly, in four out of five breast cancer cohorts for which clinical data were available, the levels of expression of chemotactic factors/receptors that exhibited universal (rather than organ-specific) positive correlations with the immune infiltrate had a positive impact on the response to neoadjuvant chemotherapy. These results support the notion that general (rather than organ-specific) rules governing the recruitment of immune cells into the tumor bed are particularly important in determining local immunosurveillance and response to therapy.

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Prediction of future metastasis and molecular characterization of head and neck squamous-cell carcinoma based on transcriptome and genome analysis by microarrays

Cited by 140 publications

References 42 publications

hsa‐miR‐210 is a marker of tumor hypoxia and a prognostic factor in head and neck cancer

hsa‐miR‐210 is a marker of tumor hypoxia and a prognostic factor in head and neck cancer

CD24 blunts oral squamous cancer development and dampens the functional expansion of myeloid-derived suppressor cells

Impact of chemotactic factors and receptors on the cancer immune infiltrate: a bioinformatics study revealing homogeneity and heterogeneity among patient cohorts

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