This research aimed to measure lower extremity kinematics for male and female youths during daily activities and to identify gender differences from kinematics of lower limbs. A total of 87 healthy participants, comprising 43 Chinese male youths and 44 female youths, were recruited from Anhui Polytechnic University to participate in this research. All participants were instructed to perform squatting activities on a soft mat starting from an upright position. Every participant completed six squatting exercises, and motion capture technology was used to gather 3D kinematic data. SPSS software was used for statistical analysis. The average 3D angles of the hip, knee, and ankle joints of male and female youths were calculated, and then the independent t-test was used for statistical significance. Data analysis showed no significant gender differences in the sagittal movements of the knee and ankle joints for Chinese male and female youths. The mean hip joint angle during squatting was [Formula: see text] for male youths and [Formula: see text] for female youths. The knee joint angle was [Formula: see text] for male youths and [Formula: see text] for female youths. With regard to ankle mobility, the hip and ankle ranges of motion were [Formula: see text] and [Formula: see text] for male youths and [Formula: see text] and [Formula: see text] for female youths. Both sexes showed significant gender differences in the nonsagittal plane. The range of motion (ROM) observed during deep squatting exceeds the ROM after arthroplasty for Chinese male and female youths. These findings contribute to a comprehensive understanding of deep squatting biomechanics for the Chinese population and provide a basis for mitigating hyperflexion exercises and fabricating appropriate joint injury prostheses. By understanding the specific movement patterns of Chinese male and female youths, healthcare professionals can develop individualized interventions and rehabilitation programs to improve functional outcomes, prevent joint injuries, and improve the overall quality of life. The research found significant gender differences for sagittal hip and anterior ankle movements of male youths during squatting. These findings shed light on the kinematics of lower limbs. They have the potential to influence future research and medical practice to improve joint health and functional mobility for Chinese male and female youths.
