Predicting the stability of freeze-dried Fusobacterium mortiferum proficiency testing samples by accelerated storage tests

Griffin, Charles W.; Cook, E C; Mehaffey, M A

doi:10.1016/0011-2240(81)90117-6

Cited by 9 publications

(4 citation statements)

References 4 publications

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“…Samples at these conditions were removed at periodic intervals and tested for quantitative recovery of gonococci. Degradation rate constants were calculated (3) for each temperature from the quantitative viability determinations. Thermal degradation should follow the logarithmic form of the Arrhenius relationship relative to absolute temperature (1); i.e., if the log of the degradation rate constant for samples at different temperatures is plotted against the reciprocal of the absolute temperature, a straight-line relationship should result.…”

Section: Methodsmentioning

confidence: 99%

“…The conventional method of assessing longterm stability by periodic testing of samples was burdensome because of the large number of tests that had to be performed; another disadvantage was the time, usually months or years, required to obtain stability data for samples stored at low temperatures. For these reasons the use of thermal degradation tests (1)(2)(3) to predict stability was studied.…”

mentioning

confidence: 99%

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Preparation and stability of freeze-dried Neisseria gonorrhoeae cultures used for external quality control

Mehaffey¹,

Cook²,

Griffin³

1984

J Clin Microbiol

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In 1976, the Centers for Disease Control initiated an external quality control program for the isolation and identification of Neisseria gonorrhoeae. This program required microbial samples of sufficient stability for shipment to laboratories throughout the United States. The Centers for Disease Control undertook studies to determine the most appropriate media for the propagation of strains for freeze-drying, the cell-suspending media that would afford protection during and after freeze-drying, the most favorable growth conditions, the proper times and methods for harvesting cells, the appropriate lyophilization conditions, the critical residual moisture content, and the stability of samples. These studies resulted in the development of methods for preparing and testing freeze-dried samples suitable for shipment.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Preparation and stability of freeze-dried Neisseria gonorrhoeae cultures used for external quality control

Mehaffey¹,

Cook²,

Griffin³

1984

J Clin Microbiol

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“…Gram pozitif bakteriler ve sporlar birçok farklı formülasyon ile başarılı bir şekilde liyofilize edilebilmektedir. Bunun tersine, Gram negatif bakteri türleri aynı şart- [11,26].…”

Section: Mikroorganizmaların Liyofilizasyona Yanıtıunclassified

“…Liyofilizasyon ile oluşabilecek mutasyonların önüne geçmek için, kurutma vasatının içerisine sülfhidril'den zengin bileşiklerin katılmasına ihtiyaç duyulabilmektedir. Ayrıca bazı hassas biyolojik materyaller ise aşırı kurutulduğunda inaktif olabilmektedirler [5,11,26].…”

Section: Mikroorganizmaların Liyofilizasyona Yanıtıunclassified

Biyolojik Maddelerin Kurutularak Saklanması: Liyofilizasyon

Ergün¹

2015

Etlik Veteriner Mikrobiyoloji Dergisi

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Organik maddelerden suyun uzaklaştırılması sürecini tanımlayan liyofilizasyon, günümüzde tüm dünyada gerek sağlık endüstrisinde ve gerekse gıda endüstrisinde çok fazla kullanım alanı bulan bir tekniktir. Bu teknikte azaltılmış basınç (vakum) altında, dondurulmuş haldeki solüsyonlardan suyun uzaklaştırılması ile biyolojik maddeler ve gıdalar, bozulma meydana gelmeden uzun süre saklanabilmektedir. Liyofilizasyonun, uzun bir süreç olması, sıcaklık ve basıncın içinde bulunduğu hassas dengeler üzerine kurulmuş olması gibi dezavantajları bulunmasına rağmen halen dünyada sıklıkla kullanılan bir yöntemdir. Bu teknik, aslen eski bir yöntem olmasına karşın biyolojik ve organik maddelerin saklanmasına ilişkin daha etkin bir teknik geliştirilemediğinden günümüzde tüm dünyada halen etkinliğini ve önemini korumaktadır. Bununla birlikte prensipte aynı olmakla birlikte liyofilizasyon teknolojisi sürekli geliştirilmektedir.Anahtar kelimeler: Liyofilizasyon, liyofilizasyon basamakları, liyofilizasyon dezavantaj/avantajı, liyofilizasyon mekanizması, liyofilizasyon metotları (dondurma, birincil kurutma, ikincil kurutma) Protection of biological materials by drying: LyophilizationSummary: Lyophilization which defines the process of evaporating the water from organic materials is a widely used technique over the world including health and food industries. This technique provides long term storing of biological materials and food by removing the water from frozen solutions under low pressure (vacuum). Although lyophilization has some disadvantages such as being a time taking process and built delicate balances involving temperature and pressure, it is still commonly used over the world. Even though it is originally an old technique, there has not been developed a more effective technique for long term storing of biological and organic materials yet. For this reason, lyophilization still protect its importance and effectiveness. Though their basic principles are the same, novel lyophilization techniques are continuously developing.

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The Principles of Freeze-Drying

Adams¹,

Cook

Ward

2014

Cryopreservation and Freeze-Drying Protocols

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This chapter provides an up-to-date overview of freeze-drying (lyophilization) with particular relevance to stabilizing live cells or viruses for industrial applications as vaccines or seed culture. The chapter discusses the importance of formulation, cycle development, validation, and the need to satisfy pharmaceutical regulatory requirements necessary for the commercial exploitation of freeze-dried products.

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Predicting the stability of freeze-dried Fusobacterium mortiferum proficiency testing samples by accelerated storage tests

Cited by 9 publications

References 4 publications

Preparation and stability of freeze-dried Neisseria gonorrhoeae cultures used for external quality control

Preparation and stability of freeze-dried Neisseria gonorrhoeae cultures used for external quality control

Biyolojik Maddelerin Kurutularak Saklanması: Liyofilizasyon

The Principles of Freeze-Drying

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