Predicting the influence of fertilization regimes on potential N fixation through their effect on free-living diazotrophic community structure in double rice cropping systems

Dai, Xianglin; Song, Dali; Guo, Qiankun; Zhou, Wei; Liu, Guangrong; Ma, Ruiping; Liang, Guozheng; Ping, He; Sun, Gang; Yuan, Fengming; Liu, Zengbing

doi:10.1016/j.soilbio.2021.108220

Cited by 38 publications

(29 citation statements)

References 73 publications

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“…However, several studies have shown that high inorganic N contents (e.g., NH 4 + -N and NO 3 − -N) signi cantly inhibit soil BNF capacity in agro-ecosystems with N fertilization addition (Dai et al 2021;Fan et al 2019), which is inconsistent with our results. A possible reason for this difference is that the soil N concentration tended to be higher in the other studies, resulting in excess N content limiting the growth and activity of microorganisms (Wang et al 2018).…”

Section: Discussioncontrasting

confidence: 99%

“…Dissolved organic matter, which can supply these microbes with additional available organic nutrients, is the major controlling factor for N cycling in soils (Levy-Booth et al 2014). N 2 xation by heterotrophic diazotrophs is highly dependent on organic substances from the environment, such as soil and plant systems; thus, it is necessary to maintain a high N 2 -xing ability in substrates with high C quantities (Dai et al 2021). In addition, the reaction of BNF is catalyzed by nitrogenase with high energy consumption, suggesting that the growth and functions of diazotrophs are not only controlled by C availability, but also by N, P, and K availability (Zheng et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…However, the research of Kumar et al (2017) showed that the nifH gene is not a good criterion for judging the potential for BNF. The function of N 2 -xation in soil is determined based on the diazotrophic community composed of multiple distinct N 2 -xation taxa (Che et al 2017;Dai et al 2021;Wang et al 2020a). Moreover, functional redundancy is widespread in microbial systems during exerting metabolic functions (Louca et al 2018).…”

Section: Introductionmentioning

confidence: 99%

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Alpine meadow degradation depresses soil nitrogen fixation by regulating plant functional groups and diazotrophic community composition

Zhang

Wang²,

Wang

et al. 2022

Plant Soil

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Biological nitrogen xation (BNF), a function performed by diazotrophic microbes, plays an essential role in nitrogen (N) bioavailability in terrestrial ecosystems. However, little is known about the effects of degradation on soil BNF and diazotrophic communities in alpine meadow. MethodsWe investigated the changes in soil BNF and their potential drivers in alpine meadows along a degradation gradient on the Tibetan Plateau (non-degraded, lightly degraded, moderately degraded, and severely degraded meadows) using real-time quantitative PCR and amplicon sequencing. ResultsSoil BNF rates decreased signi cantly along the meadow degradation gradient with a range of 17.34-79.84 nmol C 2 H 4 g − 1 dry soil d − 1 across all sites. The highest BNF was observed in the non-degraded meadow and was 1.5-4.6-fold higher than that in degraded meadows. Meadow degradation signi cantly reduced the gene abundance of nifH and the Shannon and Chao1 diversity indices of diazotrophs, accompanied by a decrease in plant biomass, soil moisture, and nutrient content (C, N component). Soil BNF potential was closely correlated with plant biomass, soil nutrient content, and diazotrophic abundance (including Nostoc, Scytonema, Rhodopseudomonas, Rhizobiales, and Proteobacteria). The community composition of diazotrophs differed markedly among sites with different levels of degradation, and both autotrophic (Cyanobacteria) and heterotrophic (Proteobacteria) diazotrophs contributed simultaneously to the BNF. The plant functional groups, especially the sedges family, were the primary drivers for soil BNF rates via mediating soil moisture, nutrient level (dissolved organic C and N), nifH gene abundance, and diazotrophic community composition. ConclusionsOur results reveal the underlying mechanism of changes in soil BNF during alpine meadow degradation, emphasize the importance of plant functional groups in shaping the diazotrophic community and BNF potential, and provide insights for the restoration of degraded meadow ecosystems.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Alpine meadow degradation depresses soil nitrogen fixation by regulating plant functional groups and diazotrophic community composition

Zhang

Wang²,

Wang

et al. 2022

Plant Soil

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“…For example, microbial C:N, a metric that highlights microbial C and N status, was negatively correlated with FLNF. This contrasts other recent studies that suggest FLNF is significantly and positively driven by C:N ratios (Dai et al, 2021; Zheng et al, 2019). In our study, N limitation alone did not seem to stimulate FLNF.…”

Section: Discussioncontrasting

confidence: 99%

“…Interestingly, Alphaproteobacteria abundance did not differ significantly by year, suggesting that individual taxa may play a large role in driving FLNF than higher level groupings. Previous work has identified Bradyrhizobium in the switchgrass rhizosphere, including as indicators of greater FLNF rates (Bahulikar et al, 2014; Dai et al, 2021; Roley et al, 2019; Smercina et al, 2020) and may suggest an important, yet overlooked role for these well‐studied symbionts in grassland systems. In general, we find weak, but significant links between the diazotroph community and FLNF rates.…”

Section: Discussionmentioning

confidence: 99%

Temporal dynamics of free‐living nitrogen fixation in the switchgrass rhizosphere

et al. 2021

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Free‐living nitrogen fixation (FLNF) represents an important terrestrial N source and is gaining interest for its potential to contribute plant available N to bioenergy cropping systems. Switchgrass, a cellulosic bioenergy crop, may be particularly reliant on FLNF when grown on low N systems, like marginal lands. However, the potential contributions of FLNF to switchgrass as well as the controls on this process are not well understood. In this study, we evaluated drivers of FLNF rates and N‐fixing microbial (diazotrophic) community composition in field‐grown switchgrass systems over two growing seasons with high temporal sampling. We found that climate variables are strong drivers of FLNF rates in switchgrass systems, compared to other environmental and biological factors including soil nutrients and diazotrophic community composition. Increased soil moisture availability generally promoted FLNF rates, but extreme rainfall events were detrimental. These climate‐related responses suggest a potential for loss of FLNF‐derived N contributions under projected climate shifts. We found a significant, but weak correlation between diazotrophic community composition and FLNF rates. We also observed a significant shift in the diazotrophic community composition between 2017 and 2018 and similarly measured a significant difference in FLNF rates between growing seasons. Lastly, we found that seasonal FLNF N contributions, based on measurement with high temporal resolution, has the potential to meet up to 80% of switchgrass N demands suggesting that FLNF measurements extrapolated from fewer time points or locations may underestimate these potential N contributions.

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Synergistic effects of diazotrophs and arbuscular mycorrhizal fungi on soil biological nitrogen fixation after three decades of fertilization

Zhou

Fan

et al. 2023

iMeta

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Biological nitrogen (N) fixation (BNF) via diazotrophs is an important ecological process for the conversion of atmospheric N to biologically available N. Although soil diazotrophs play a dominant role in BNF and arbuscular mycorrhizal fungi (AMF) serve as helpers to favor BNF, the response of soil BNF and diazotrophic communities to different long-term fertilizations and the role of AMF in diazotrophs-driven BNF are poorly understood. Herein, a 33-year fertilization experiment in a wheat-maize intercropping system was conducted to investigate the changes in soil BNF rates, diazotrophic and AMF communities, and their interactions after long-term representative fertilization (chemical fertilizer, cow manure, wheat straw, and green manure). We found a remarkable increase in soil BNF rates after more than three decades of fertilization compared with nonfertilized soil, and the green manure treatment rendered the highest enhancement. The functionality strengthening was mainly associated with the increase in the absolute abundance of diazotrophs and AMF and the relative abundance of the key ecological cluster of Module #0 (gained from the co-occurrence network of diazotrophic and AMF species) with dominant diazotrophs such as Skermanella and Azospirillum. Furthermore, although the positive correlations between diazotrophs and AMF were reduced under long-term organic fertilization regimes, green manuring could reverse the decline within Module #0, and this had a positive relationship with the BNF rate. This study suggests that long-term fertilization could promote N fixation and select specific groups of N fixers and their helpers in certain areas. Our work provides solid evidence that N fixation and certain groups of diazotrophic and AMF taxa and their interspecies relationship will be largely favored after the fertilized strategy of green manure.

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Predicting the influence of fertilization regimes on potential N fixation through their effect on free-living diazotrophic community structure in double rice cropping systems

Cited by 38 publications

References 73 publications

Alpine meadow degradation depresses soil nitrogen fixation by regulating plant functional groups and diazotrophic community composition

Alpine meadow degradation depresses soil nitrogen fixation by regulating plant functional groups and diazotrophic community composition

Temporal dynamics of free‐living nitrogen fixation in the switchgrass rhizosphere

Synergistic effects of diazotrophs and arbuscular mycorrhizal fungi on soil biological nitrogen fixation after three decades of fertilization

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