Camellia oleifera Abel., is an important woody plant, and its fruit contains high-quality edible oil. Production of C. oleifera has significantly expanded over the last 20 years in China. Due to the lack of appropriate information on nutrient management, its production has encountered low yield and low oil quality problems. As nitrogen (N) is an essential nutrient and the most abundant in C. oleifera tissues, the present study investigated effects of different ratios of nitrate (NO3â) and ammonium (NH4+) on the growth of a cultivar Xianglin 27 at the seedling stage. Uniform seedlings were grown in a soil-based substrate in containers and fertigated with solutions composed of six ratios of NO3â and NH4+, respectively for five months. Results showed that C. oleifera prefers both NO3â and NH4+ at a ratio of 1:1. Seedlings receiving this solution had the highest total N in leaves and total dry weight; elevated chrolophyll, soluble saccharide and protein contents as well as higher activities of peroxidase (POD), superoxide dismutase (SOD), nitrate reductase (NR), glutamine synthetase (GS), and glutamate synthase (GOGAT). Our study shows for the first time that N supply for producing C. oleifera should be an equal ratio of NO3â and NH4+.