Predicting plant immunity gene expression by identifying the decoding mechanism of calcium signatures

Lenzoni, Gioia; Liu, Junli; Knight, Marc R.

doi:10.1111/nph.14924

Cited by 40 publications

(35 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this context, the absence of PLC2 in cAMP‐sponge expressing lines could be involved in the failure of Ca 2+ entrance into cells. Lacking the correct Ca 2+ modulation, plants could not be able to activate the suitable response to a given stress, and consequently do not acclimate to the new conditions, compromising their survival (Lenzoni et al ., ). Accordingly, the delayed raise in cytosolic Ca 2+ would not allow cAS plants to initiate the suitable defence response, consequently affecting resistance.…”

Section: Discussionmentioning

confidence: 97%

Genetic buffering of cyclic AMP in Arabidopsis thaliana compromises the plant immune response triggered by an avirulent strain of Pseudomonas syringae pv. tomato

et al. 2019

View full text Add to dashboard Cite

Cyclic AMP plays important roles in different physiological processes, including plant defence responses. However, as little information is known on plant enzymes responsible for cAMP production/degradation, studies of cAMP functions have relied, to date, on non-specific pharmacological approaches. We therefore developed a more reliable approach, producing transgenic Arabidopsis thaliana lines overexpressing the 'cAMP-sponge' (cAS), a genetic tool that specifically buffers cAMP levels. In response to an avirulent strain of Pseudomonas syringae pv. tomato (PstAvrB), cAS plants showed a higher bacterial growth and a reduced hypersensitive cell death in comparison with wild-type (WT) plants. The low cAMP availability after pathogen infection delayed cytosolic calcium elevation, as well as hydrogen peroxide increase and induction of redox systems. The proteomic analysis, performed 24 h post-infection, indicated that a core of 49 proteins was modulated in both genotypes, while 16 and 42 proteins were uniquely modulated in WT and cAS lines, respectively. The involvement of these proteins in the impairment of defence response in cAS plants is discussed in this paper. Moreover, in silico analysis revealed that the promoter regions of the genes coding for proteins uniquely accumulating in WT plants shared the CGCG motif, a target of the calcium-calmodulinbinding transcription factor AtSR1 (Arabidopsis thaliana signal responsive1). Therefore, following pathogen perception, the low free cAMP content, altering timing and levels of defence signals, and likely acting in part through the mis-regulation of AtSR1 activity, affected the speed and strength of the immune response. Role of cAMP in plant immune response 597Generation of Arabidopsis cAS-mCherry plants expressing the NES-YC3.6 probeThe Arabidopsis Col-0 cAS-transgenic lines were crossed with the Col-0 pUBQ10-NES-YC3.6 line reported in Krebs et al. (2012). Seeds from cross-pollinated flowers were surface sterilized by vapour-

show abstract

Section: Discussionmentioning

confidence: 97%

Genetic buffering of cyclic AMP in Arabidopsis thaliana compromises the plant immune response triggered by an avirulent strain of Pseudomonas syringae pv. tomato

et al. 2019

View full text Add to dashboard Cite

show abstract

“…To date, it has not yet been elucidated how CaM can balance the activation of CBP60g and/or CAMTA3 upon pathogen attack with how the Ca 2+ signatures are integrated in this transcriptional pathway. Efforts are being made in this perspective and dynamic mathematical models incorporating several parameters are being developed with the aim of predicting regulation networks according to the generated Ca 2+ signatures [ 87 , 88 ].…”

Section: Ca 2+ Decoding Processes and Plant Immmentioning

confidence: 99%

“…Although the CaM contribution in plant immunity was mainly revealed by the identification of CaMBP, it is now clear how CaM plays a pivotal role in the fine tuning of immune responses by acting either as a positive or a negative regulator of defence responses. Identification of the whole set of CaMBPs is certainly not complete and other CaM-binding TFs including several members of TGA, WRKY, MYB and NAC families also contribute to plant immunity either positively or negatively [ 63 , 88 , 89 ]. For example, TGA3 and several WRKY transcription factors such as WRKY7 and WRKY53 can interact with CaM in a Ca 2+ -dependent manner [ 68 ] but the effects of this interaction on the physiological and/or biochemical function of these TFs remain unknown.…”

Section: Ca 2+ Decoding Processes and Plant Immmentioning

confidence: 99%

Calcium Signalling in Plant Biotic Interactions

Aldon

Mbengue

Mazars

et al. 2018

IJMS

247

137

View full text Add to dashboard Cite

Calcium (Ca2+) is a universal second messenger involved in various cellular processes, leading to plant development and to biotic and abiotic stress responses. Intracellular variation in free Ca2+ concentration is among the earliest events following the plant perception of environmental change. These Ca2+ variations differ in their spatio-temporal properties according to the nature, strength and duration of the stimulus. However, their conversion into biological responses requires Ca2+ sensors for decoding and relaying. The occurrence in plants of calmodulin (CaM) but also of other sets of plant-specific Ca2+ sensors such as calmodulin-like proteins (CMLs), Ca2+-dependent protein kinases (CDPKs) and calcineurin B-like proteins (CBLs) indicate that plants possess specific tools and machineries to convert Ca2+ signals into appropriate responses. Here, we focus on recent progress made in monitoring the generation of Ca2+ signals at the whole plant or cell level and their long distance propagation during biotic interactions. The contribution of CaM/CMLs and CDPKs in plant immune responses mounted against bacteria, fungi, viruses and insects are also presented.

show abstract

“…Biotic and abiotic stresses (including mechanical, salt, osmotic, and oxidative stress) trigger rapid and transient modulations in cytosolic and organellar free Ca 2+ (Knight et al, 1991(Knight et al, , 1992(Knight et al, , 1997aKiegle et al, 2000;Monshausen et al, 2009;Loro et al, 2012Loro et al, , 2016Bonza et al, 2013;Laohavisit et al, 2013;Behera et al, 2018;Manishankar et al, 2018). These Ca 2+ signatures are held to be specific to the stimulus and result in stimulusspecific outcomes, enabled by suites of decoding proteins (Whalley et al, 2011;Whalley and Knight, 2013;Liu et al, 2015;Lenzoni et al, 2018).…”

mentioning

confidence: 99%

Phosphate Starvation Alters Abiotic-Stress-Induced Cytosolic Free Calcium Increases in Roots

et al. 2019

View full text Add to dashboard Cite

Phosphate (Pi) deficiency strongly limits plant growth, and plant roots foraging the soil for nutrients need to adapt to optimize Pi uptake. Ca 2+ is known to signal in root development and adaptation but has to be tightly controlled, as it is highly toxic to Pi metabolism. Under Pi starvation and the resulting decreased cellular Pi pool, the use of cytosolic free Ca 2+ ([Ca 2+ ] cyt) as a signal transducer may therefore have to be altered. Employing aequorin-expressing Arabidopsis (Arabidopsis thaliana), we show that Pi starvation, but not nitrogen starvation, strongly dampens the [Ca 2+ ] cyt increases evoked by mechanical, salt, osmotic, and oxidative stress as well as by extracellular nucleotides. The altered root [Ca 2+ ] cyt response to extracellular ATP manifests during seedling development under chronic Pi deprivation but can be reversed by Pi resupply. Employing ratiometric imaging, we delineate that Pi-starved roots have a normal response to extracellular ATP at the apex but show a strongly dampened [Ca 2+ ] cyt response in distal parts of the root tip, correlating with high reactive oxygen species levels induced by Pi starvation. Excluding iron, as well as Pi, rescues this altered [Ca 2+ ] cyt response and restores reactive oxygen species levels to those seen under nutrient-replete conditions. These results indicate that, while Pi availability does not seem to be signaled through [Ca 2+ ] cyt , Pi starvation strongly affects stress-induced [Ca 2+ ] cyt signatures. These data reveal how plants can integrate nutritional and environmental cues, adding another layer of complexity to the use of Ca 2+ as a signal transducer.

show abstract

Predicting plant immunity gene expression by identifying the decoding mechanism of calcium signatures

Cited by 40 publications

References 42 publications

Genetic buffering of cyclic AMP in Arabidopsis thaliana compromises the plant immune response triggered by an avirulent strain of Pseudomonas syringae pv. tomato

Genetic buffering of cyclic AMP in Arabidopsis thaliana compromises the plant immune response triggered by an avirulent strain of Pseudomonas syringae pv. tomato

Calcium Signalling in Plant Biotic Interactions

Phosphate Starvation Alters Abiotic-Stress-Induced Cytosolic Free Calcium Increases in Roots

Contact Info

Product

Resources

About