“…The value of prospective, longitudinal, follow-up studies, ideally starting before conception, and extending through pregnancy and birth into childhood and beyond, is emphasized. For these studies, deployment of state-of-the art methods, including 3D/4D fetal ultrasonography for quantification of fetal growth (biometry), regional blood flow (uterine, umbilical, cerebral, hepatic and renal [109,110]), volume and growth trajectory of organs (placenta, brain, liver, kidneys, adrenals) [111,112], and body composition (arm, thigh and visceral fat/lean mass [113•, 114]), coupled with reliable assessments in newborns, infants, and children of body composition [with magnetic resonance imaging (MRI) or dual energy X-ray absorptiometry; DXA] and energy expenditure [total energy expenditure using the doubly labeled water method (DLW), and resting metabolic state using the indirect calorimetry approach], will move the field forward in an informed manner. Furthermore, recent advances in imaging techniques will likely enable the developments of protocols in infants and children for subcutaneous and visceral fat quantification (especially intrahepatic fat) [115•], and characterization of and differentiation between white and brown adipose tissue [116••].…”