Predicting early pregnancy in Egyptian buffalo cows via measuring uterine and luteal blood flows, and serum and saliva progesterone

Lasheen, Mohamed E.; Badr, Hussein M.; Kandiel, Mohamed M.M.; El‐Maaty, Amal M. Abo; Samir, Haney; Farouk, Mohammed Hamdy; Eldawy, Mohamed H.

doi:10.1007/s11250-017-1413-6

Cited by 21 publications

(17 citation statements)

References 33 publications

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“…In our study, pregnant buffaloes showed, at Day 10 post-AI, higher BFA and P 4 blood concentration (P < 0.01) but not higher CLS compared to their not-pregnant counterparts. This latter finding is in agreement with another study (13), in which no differences in the CL area were recorded during this period (Day 5-10) between pregnant and not-pregnant buffaloes. It is already known that, since Day 5 post-TAI, buffaloes that showed lower CL vascularization had a lower pregnancy rate (7).…”

Section: Discussionsupporting

confidence: 93%

“…The color Doppler ultrasound imaging approach has the potential to improve buffalo reproductive efficiency by allowing for earlier resynchronization of open females and, consequently, a reduction in the interval between services (5,13). Moreover, the use of a free post-processing tool such as ImageJ could be of great advantage for management strategies that use intensive resynchronization schedules to improve reproductive performance.…”

Section: Introductionmentioning

confidence: 99%

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Early Prediction of Corpus Luteum Functionality Using an Imaging Software

et al. 2020

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The present study aimed to assess the applicability of luteal blood flow data acquired through the use of color Doppler ultrasonography and a post-processing analysis tool (ImageJ) for predicting pregnancy in buffaloes (Bubalus bubalis). The experiment was carried out on 59 multiparous Italian Mediterranean buffaloes that underwent synchronization of estrus and fixed-time artificial insemination (TAI). Corpus luteum features (size: CLS and blood flow: BFA) were taken from Day 5 to 10 after TAI and retrospectively measured with ImageJ. In the same period, blood samples were taken to assess progesterone (P 4) concentrations. Pregnancy diagnosis was carried out on Day 45 by ultrasound and confirmed on Day 70 post-TAI. Differences in CLS, BFA, and P 4 concentrations from Day 5 to 10 after TAI measured between groups were analyzed by ANOVA repeated measures as were differences within each day of measuring. Buffaloes that established a pregnancy (n = 29; 55%) had larger CLS (2.2 ± 0.1 vs. 1.9 ± 0.1 cm 2 ; P < 0.01), higher BFA (0.6 ± 0.0 vs. 0.4 ± 0.0 cm 2 ; P < 0.01), and higher P 4 blood level (1.8 ± 0.1 vs. 1.4 ± 0.1; P < 0.01) during Day 5-10 as compared to not-pregnant buffaloes (n = 22). Throughout the entire period, the first feature that changed between groups was P 4 blood concentration at Day 7 (1.7 ± 0.1 vs. 1.2 ± 0.1; P < 0.05) followed by BFA at Day 8 (0.6 ± 0.0 vs. 0.5 ± 0.0; P < 0.05), respectively, in pregnant and not-pregnant animals. The ROC analyses indicated that P 4 was able to predict pregnancy since Day 5 (P < 0.05) although a more reliable result could be obtained from Day 8 (P < 0.01). At Day 10, it was possible to set a cutoff value for every parameter taken into account. The logistic regression analysis showed that pregnancy was positively influenced by P 4 concentration (odds ratio 534.127; P < 0.01) and BFA (odds ratio 744.893; P < 0.01). In conclusion, the use of color Doppler ultrasonography, together with ImageJ, identified different patterns of BFA between pregnant and not-pregnant buffaloes starting from Day 8 post-TAI.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Early Prediction of Corpus Luteum Functionality Using an Imaging Software

et al. 2020

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“…While in some studies, pregnant buffaloes showed a higher CL area compared to nonpregnant subjects [ 10 , 27 ], no differences were observed in other studies [ 28 , 29 ]. In a recent trial [ 30 ], a difference in CL dimensions between pregnant and nonpregnant buffaloes was observed only 14 days after insemination, while no differences were recorded during the Day 5 to Day 10 period. Therefore, further investigations are needed to clarify this aspect.…”

Section: Discussionmentioning

confidence: 99%

Corpus Luteum Color Doppler Ultrasound and Pregnancy Outcome in Buffalo during the Transitional Period

Esposito

Salzano

Russo

et al. 2020

Animals

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This study evaluated corpus luteum (CL) development in buffaloes out of breeding season and assessed an early pregnancy diagnosis. Mediterranean buffaloes (n = 29) were synchronized and artificially inseminated. CL B-mode/color Doppler ultrasonography examinations were performed daily from Days 5 to 10 post-synchronization, recording CL dimensions and blood flow parameters. Blood samples were collected on the same days for the progesterone (P4) assay. Data were grouped into pregnant or nonpregnant and retrospectively analyzed. The total pregnancy rate was 50.0% (13/26) on Day 45. A significant difference between CL average area in pregnant and nonpregnant buffaloes was recorded only on Day 10. Pregnant buffaloes showed a significantly higher mean P4 concentration and higher mean time average medium velocity (TAMV) values from Day 5 to Day 10 compared to nonpregnant buffaloes. Linear regression analysis showed a significant relationship between P4 levels and TAMV. Multiple logistic regression highlighted a significant influence of TAMV on pregnancy outcome, particularly on Day 8. This is probably due to the strong relationship between TAMV and P4 production. Both TAMV and P4 could be used to predict pregnancy starting on Day 6, although a more reliable result was obtained at Day 10. Thus, the period between Days 5 and 10 is critical for CL development during the transitional period in buffalo.

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“…The left panel is by B-mode ultrasonography (A, C, E, G), while the right panel is representing the color (B, F) and power Doppler modes (D, H).). Color and power Doppler images were analyzed for the blood flow perfusions area (s) in pixels using an image analysis program (Adobe Photoshop CC software, Adobe Systems, USA) as described in buffaloes [13]. Blood flow which is colored either blue (away from the transducer) or red (toward the transducer), and total color, and power areas were counted.…”

Section: Methodsmentioning

confidence: 99%

Ovarian follicular changes and hemodynamics in Egyptian buffaloes under CIDR-PGF2α and Ovsynch-CIDR estrus synchronization treatments

Samir

Kandiel

El‐Maaty

et al. 2019

Journal of Reproduction and Development

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This study aimed at investigating the efficacy of two protocols of estrous synchronization on follicular changes and hemodynamics. Pluriparous Egyptian buffaloes (n = 36) were synchronized either with controlled internal drug release (CIDR)-PGF2α (7-days CIDR insert with PGF2α injected on the 6th day; n = 18) or Ovsynch-CIDR (Ovsynch protocol concurrent with 7-days CIDR insert; n = 18). Blood sampling and ovarian ultrasound examinations (Grayscale, color and power Doppler modes) were conducted on the Day of CIDR removal, estrus, and luteal phase. Mean follicle diameter (MFD), first (1st-LF) and second (2nd-LF) largest follicle diameters, and E2 levels significantly increased in the CIDR-PGF2α group at CIDR withdrawal. Ovsynch-CIDR markedly fortified higher follicle population, MFD, and 1st-LF diameter at estrus and corpus luteum (CL) volume at the luteal phase in concomitant with increases (P < 0.05) in E2 (at estrus) and P4 (at luteal phase). At CIDR removal, the blue pixels in the dominant follicle (DF) were higher (1.5 times; P = 0.054) in the Ovsynch-CIDR than in the CIDR-PGF2α. At estrus, total blood flow (TBF) and power Doppler pixels (PDP) to DF(s) were noticeably higher (seven and 1.6 times; respectively) in the Ovsynch-CIDR than in CIDR-PGF2α (5906 ± 237 vs. 830 ± 60 pixels, P < 0.01 and 5479 ± 322 vs. 3377 ± 19 pixels, P < 0.05; respectively). At the luteal phase, TBF and PDP to the CL increased in the Ovsynch-CIDR group than in the CIDR-PGF2α group (11060 ± 965 vs. 7963 ± 480 pixels, 1.4 times, P = 0.05 and 18900 ± 1350 vs. 13220 ± 568 pixels, 1.1 times, P = 0.005; respectively). In conclusion, based on the improvement in synchronized follicular activity and hemodynamics, the Ovsynch-CIDR regimen is persuaded in Egyptian buffaloes.

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Predicting early pregnancy in Egyptian buffalo cows via measuring uterine and luteal blood flows, and serum and saliva progesterone

Cited by 21 publications

References 33 publications

Early Prediction of Corpus Luteum Functionality Using an Imaging Software

Early Prediction of Corpus Luteum Functionality Using an Imaging Software

Corpus Luteum Color Doppler Ultrasound and Pregnancy Outcome in Buffalo during the Transitional Period

Ovarian follicular changes and hemodynamics in Egyptian buffaloes under CIDR-PGF2α and Ovsynch-CIDR estrus synchronization treatments

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