Predicting Critical Duration and Reversibility of Damage in Acute Mesenteric Ischemia: an experimental study

Abstract

BACKGROUND:The objective of the current study was to investigate the value of the ischemic biomarkers endothelial cell-specific molecule-1 (endocan) and signal peptide-CUB-EGF domain-containing protein-1 (SCUBE-1) in the diagnosis and assessment of earlystage and irreversible damage in acute mesenteric ischemia. METHODS:An experimental mesenteric ischemia reperfusion model was designed using 54 rats. Nine groups were created: Three sham groups [Groups I (30 th minute), IV (2 nd hour), and VII (6 th hour)], in which only blood and tissue specimens were sampled; 3 ischemia groups [Groups II (30 th minute), V (2 nd hour), and VIII (6 th hour)], in which blood and tissue specimens were sampled after ligation of the superior mesenteric artery (SMA); and 3 reperfusion groups [Groups III (30 th minute), VI (2 nd hour), and IX (6 th hour)], in which blood and tissue specimens were sampled after declamping the SMA and reperfusion for 1 hour. SCUBE-1 and endocan samples obtained from blood and tissue were examined histopathologically. RESULTS:The SCUBE-1 level was higher in the ischemia groups when compared with the sham groups (p<0.05), and the endocan level was markedly different in the late ischemia (6 th hour) group. When these 2 markers were used together to assess irreversible mesenteric damage in the histopathological examination, the sensitivity in distinguishing between reversible or irreversible damage was 94.1% with a specificity of 73.7%. CONCLUSION:The elevation of SCUBE-1 alone seems to be significant for predicting early mesenteric ischemia in laboratory rats. The combination of SCUBE-1 and endocan may be useful to detect irreversible intestinal damage.

The potential of SCUBE-1 levels as a biomarker for placental malfunction in preeclampsia

“…SCUBE-1 is a protein from the SCUBE protein family that is increased in in ammatory processes and hypoxia-related conditions (14). In the literature, it has been reported that SCUBE-1 levels increase during oxidative stress such as ischemic events, mesenteric ischemia (15), testicular torsion (16), acute coronary syndrome (8), pulmonary embolism (17), cancer diseases (18).…”

Biomarkers In Prediction of Acute Mesenteric Ischaemia: a prospective multicentre study (BIPAMI study): a study protocol

“…Serum inflammatory markers interleukin-6 (IL-6) and procalcitonin (PCT), ischaemia modified albumin (IMA) and alpha-glutathione S transferase (alpha-GST) have shown relatively high predictive values for diagnosis of any type (transmural or not transmural) of AMI in humans [ 4 ]. Of coagulation factors, D-dimers performed moderately well in predicting transmural AMI in humans [ 4 ], while signal peptide-CUB-EGF domain-containing protein 1 (SCUBE-1) predicted early mesenteric ischaemia in animal experiments [ 5 , 6 ]. The enterocyte injury marker intestinal fatty acid-binding protein (I-FABP) has been extensively studied in humans and has shown moderate diagnostic accuracy for diagnosis of AMI [ 4 ].…”

Evaluation of protective effect of L-carnitine and N-acetylcysteine in mesenteric ischemia-reperfusion injury model in rats

“…Mesenteric ischemia is a vascular emergency condition with a high potential for fatal progression [1]; therefore, early diagnosis and treatment are essential. Although diagnostic methods have recently improved, the diagnosis of this condition depends on clinical suspicion [2].…”