Predicting chemoresistance in human malignant glioma cells: The role of molecular genetic analyses

Weller, Michael; Rieger, Johannes; Grimmel, Cornelia; Meir, Erwin G. Van; Tribolet, Nicolas de; Krajewski, Stanisław; Reed, John C.; Deimling, Andreas von; Dichgans, J.

doi:10.1002/(sici)1097-0215(19981218)79:6<640::aid-ijc15>3.3.co;2-q

Cited by 28 publications

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“…15,29 The IC 50 values for the cell lines tested ranged from 137 to 162 nM. Although these cell lines have diverse molecular genetic features, with various combinations of abnormalities in the p53, PTEN, MDM2, p14, and p16 genes, 33 there was minimal difference between cell lines in the magnitude of effects observed. Our observation of a concentration-dependent cyto-toxic effect against glioma cells was confirmed using a series of clonogenic assays, which showed a steep decline in colony-forming activity at nanomolar concentrations of TSA.…”

Section: Discussionmentioning

confidence: 99%

Effect of trichostatin A, a histone deacetylase inhibitor, on glioma proliferation in vitro by inducing cell cycle arrest and apoptosis

Wetzel

Premkumar²,

Arnold³

et al. 2005

Journal of Neurosurgery: Pediatrics

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The TSA caused inhibition of glioma cell growth by both cell cycle arrest and apoptosis. Cell cycle arrest was associated with an increase in p21WAF1 expression and a decrease in phosphorylated Rb. Apoptosis was mediated at least partly through the activation of caspase-3. Because of the differential effects in glioma cells compared with nonneoplastic cells, TSA may provide a novel strategy for achieving tumor growth inhibition and cytotoxicity. Further investigation is warranted.

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Section: Discussionmentioning

confidence: 99%

Effect of trichostatin A, a histone deacetylase inhibitor, on glioma proliferation in vitro by inducing cell cycle arrest and apoptosis

Wetzel

Premkumar²,

Arnold³

et al. 2005

Journal of Neurosurgery: Pediatrics

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“…21 The cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum, glutamine (2 mM), and penicillin (100 IU/ml)/streptomycin (100 g/ml).…”

Section: Cell Lines and Cell Culturementioning

confidence: 99%

“…21 The following primary antibodies were used at concentrations of 1 to 2 g/ml: mouse monoclonal anti-MMP-2, anti-MMP-3, anti-MMP-7, anti-MMP-9, anti-MT1-MMP, and anti-TIMP-2; mouse monoclonal anti-BCL2 and rabbit polyclonal anti-BAK and anti-BAX (N20); and mouse monoclonal anti-BCL-XL, anti-BAD, and anti-MCL-1. The protein bands were visualized using antimouse IgG or anti-rabbit IgG and the enhanced chemiluminescence system.…”

Section: Sodium Dodecyl Sulfate-polyacrylamide Gel Electrophoresis Mementioning

confidence: 99%

“…We asked whether an assessment of endogenous BCL-2 family protein expression could allow us to predict the migratory and invasive potential of glioma cell lines. Although BCL-2 family protein expression was examined and reported 2 years ago, 21 we believed that it was necessary to reassess these data precisely with the cells that were used for the experiments reported herein. The levels for the antiapoptotic proteins BCL-2, BCL-XL, and MCL-1 were rather variable among the cell lines, as were the levels of the proapoptotic BAK and BAD proteins.…”

Section: Protein Levels Of the Bcl-2 Family: Relationship With Mmp Mmentioning

confidence: 99%

“…21 The U87MG, D247MG, and U138MG cell lines have wild-type p53; the LN-229 cell line is heterozygous but exhibits functional p53 activity, and the status of p53 is therefore regarded as wild type in this study. The other eight cell lines have mutant or deleted p53.…”

Section: Status Of P53 Modulates Migration Of Glioma Cellsmentioning

confidence: 99%

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Molecular determinants of glioma cell migration and invasion

Wild-Bode¹,

Weller²,

Wick

2001

Journal of Neurosurgery

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120

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Chromosomal imbalances associated with response to chemotherapy and cytotoxic cytokines in human malignant glioma cell lines

et al. 2001

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The median survival for human malignant glioma patients treated with neurosurgery and postoperative radiotherapy does not exceed one year. Only a minority of patients benefit from adjuvant chemotherapy. It was the aim of our study to determine which genomic alterations in malignant gliomas modulate the sensitivity to chemotherapy or cytotoxic cytokines such as CD95 ligand (CD95L) or Apo2L/tumor necrosis factor‐related apoptosis‐inducing ligand (Apo2L/TRAIL). Therefore, we analyzed 12 human malignant glioma cell lines for chromosomal gains and losses by comparative genomic hybridization (CGH). The gains most commonly identified were on chromosomes 7q, 19, 1, and 20q, whereas the most frequent losses were on 13q, 11q, 18q, and 4q. By comparison with previously published data on this panel of glioma cell lines11, 12, we defined candidate regions which may carry genes responsible for sensitivity to chemotherapy or cytotoxic cytokines. All but one of the chromosomal regions associated with response to chemotherapy, i.e. 1p12, 3p21, 11p11.2–p13, 12q23, 17p11.2–p13, were different from those associated with response to cytotoxic cytokines, i.e. lp12, 1q22, 12q12–q21. Genomic regions known to harbor major candidate genes, including genes encoding death ligands, death receptors, caspases and BCL‐2 family proteins, were not found to be imbalanced. In addition, we identified 5q13–q14, 5q34, 10p11.2, 9q21–q34 as genomic regions associated with the proliferative activity of malignant glioma cell lines. Cell lines with gain on proximal 5q, where CCNB1 and CCNH reside, showed an increased growth rate, suggesting that cyclins activating cdc2, the dominant G2/M phase kinase, may play a role in glioma tumorigenes. © 2001 Wiley‐Liss, Inc.

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Predicting chemoresistance in human malignant glioma cells: The role of molecular genetic analyses

Cited by 28 publications

References 0 publications

Effect of trichostatin A, a histone deacetylase inhibitor, on glioma proliferation in vitro by inducing cell cycle arrest and apoptosis

Effect of trichostatin A, a histone deacetylase inhibitor, on glioma proliferation in vitro by inducing cell cycle arrest and apoptosis

Molecular determinants of glioma cell migration and invasion

Chromosomal imbalances associated with response to chemotherapy and cytotoxic cytokines in human malignant glioma cell lines

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