Predicting Anatomical Therapeutic Chemical (ATC) Classification of Drugs by Integrating Chemical-Chemical Interactions and Similarities

Fang

Journal of Biomolecular Structure and Dynamics

et al. 2015

Self Cite

130

A microRNA (miRNA) is a small non-coding RNA molecule, functioning in transcriptional and post-transcriptional regulation of gene expression. The human genome may encode over 1000 miRNAs. Albeit poorly characterized, miRNAs are widely deemed as important regulators of biological processes. Aberrant expression of miRNAs has been observed in many cancers and other disease states, indicating that they are deeply implicated with these diseases, particularly in carcinogenesis. Therefore, it is important for both basic research and miRNA-based therapy to discriminate the real pre-miRNAs from the false ones (such as hairpin sequences with similar stem-loops). Particularly, with the avalanche of RNA sequences generated in the post-genomic age, it is highly desired to develop computational sequence-based methods for effectively identifying the human pre-miRNAs. Here, we propose a predictor called "iMiRNA-PseDPC", in which the RNA sequences are formulated by a novel feature vector called "pseudo distance-pair composition" (PseDPC) with 10 types of structure statuses. Rigorous cross-validations on a much larger and more stringent newly constructed benchmark data-set showed that our approach has remarkably outperformed the existing ones in either prediction accuracy or efficiency, indicating the new predictor is quite promising or at least may become a complementary tool to the existing predictors in this area. For the convenience of most experimental scientists, a user-friendly web server for the new predictor has been established at http://bioinformatics.hitsz.edu.cn/iMiRNA-PseDPC/, by which users can easily get their desired results without the need to go through the mathematical details. It is anticipated that the new predictor may become a useful high throughput tool for genome analysis particularly in dealing with large-scale data.

Section: Metrics For Validation and Evaluationmentioning

confidence: 99%

iMiRNA-PseDPC: microRNA precursor identification with a pseudo distance-pair composition approach

Fang

Journal of Biomolecular Structure and Dynamics

et al. 2015

Self Cite

130

“…[28] The set of the above metrics is valid only for the single-label systems. For the multi-label systems whose existence has become more frequent in system biology [81][82] and system medicine, [83][84] a different set of metrics as defined in Reference [85] is needed. The proposed methods were first evaluated on the benchmark dataset (see Supporting Information S3), which is similar to the dataset used by DNAbinder.…”

Section: Evaluation Methodologymentioning

confidence: 99%

PseDNA‐Pro: DNA‐Binding Protein Identification by Combining Chou’s PseAAC and Physicochemical Distance Transformation

Fan

et al. 2014

Molecular Informatics

154

112

Identification of DNA-binding proteins is an important problem in biomedical research as DNA-binding proteins are crucial for various cellular processes. Currently, the machine learning methods achieve the-state-of-the-art performance with different features. A key step to improve the performance of these methods is to find a suitable representation of proteins. In this study, we proposed a feature vector composed of three kinds of sequence-based features, including overall amino acid composition, pseudo amino acid composition (PseAAC) proposed by Chou and physicochemical distance transformation. These features not only consider the sequence composition of proteins, but also incorporate the sequence-order information of amino acids in proteins. The feature vectors were fed into Support Vector Machine (SVM) for DNA-binding protein identification. The proposed method is called PseDNA-Pro. Experiments on stringent benchmark datasets and independent test datasets by using the Jackknife test showed that PseDNA-Pro can achieve an accuracy of higher than 80 %, outperforming several state-of-the-art methods, including DNAbinder, DNA-Prot, and iDNA-Prot. These results indicate that the combination of various features for DNA-binding protein prediction is a suitable approach, and the sequence-order information among residues in proteins is relative for discrimination. For practical applications, a web-server of PseDNA-Pro was established, which is available from http://bioinformatics.hitsz.edu.cn/PseDNA-Pro/.

“…Compared to the Jackknife test [39], [40], the 10-fold cross-validation test requires less computing time and provides similar results for a given dataset. Therefore, the current study adopted this cross-validation method to evaluate the performance of the prediction method.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Tumor Suppressor Genes Based on Gene Ontology and the KEGG Pathway

et al. 2014

Self Cite

Cancer is a serious disease that causes many deaths every year. We urgently need to design effective treatments to cure this disease. Tumor suppressor genes (TSGs) are a type of gene that can protect cells from becoming cancerous. In view of this, correct identification of TSGs is an alternative method for identifying effective cancer therapies. In this study, we performed gene ontology (GO) and pathway enrichment analysis of the TSGs and non-TSGs. Some popular feature selection methods, including minimum redundancy maximum relevance (mRMR) and incremental feature selection (IFS), were employed to analyze the enrichment features. Accordingly, some GO terms and KEGG pathways, such as biological adhesion, cell cycle control, genomic stability maintenance and cell death regulation, were extracted, which are important factors for identifying TSGs. We hope these findings can help in building effective prediction methods for identifying TSGs and thereby, promoting the discovery of effective cancer treatments.