Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia

Suárez‐Cuenca, Juan Antonio; Vera-Gómez, Eduardo; Hernández-Patricio, Alejandro; Ruíz-Hernández, Atzin Suá; Gutiérrez-Buendía, Juan Ariel; Zamora-Alemán, Carlos Ramiro; Melchor-López, Alberto; Rizo-García, Yasser Alberto; Lomán-Zúñiga, Oscar Antonio; Escotto-Sánchez, Ignacio; Rodríguez-Trejo, Juan Miguel; Vaca, Rebeca Pérez‐Cabeza de; Téllez-González, Mario Antonio; Mondragón-Terán, Paul

doi:10.3791/61503-v

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Suarez-Cuenca et al 6 presented a protocol for the quantification of circulating mononuclear progenitor cells (MPCs) from vessels close to the angioplasty site, in order to predict the risk of limb amputation in patients with critical limb ischemia (CLI). This is another interesting topic, since the risk of amputation in CLI nowadays is still very high 7 , and there is a substantial lack of available predictive models 8 .…”

mentioning

confidence: 99%

Research Methodologies In Vascular Disease

Fittipaldi

Francesco²

2022

JoVE

View full text Add to dashboard Cite

The main purpose of the present collection is to provide readers with up-to-date knowledge on the new methodology to study vascular disease on cell, protein, and mRNA levels, both in vivo and in vitro, in humans or in a mouse model. This includes new imaging and artificial intelligence modalities, as well as histopathological analysis extended to difficult tissue retrieval 1 , like calcified arteries. The collection presents four methods that fully answer these questions, providing very interesting cues on how to approach old issues with a modern and technological perspective, showing a rapid technical evolution in this field. Ischemia-reperfusion injury (IRI) is a major risk factor for acute kidney injury (AKI) 2 . No specific diagnostic biomarkers have been established, although miRNAs are excellent candidates 3 . Aomatsu et al. 4 developed an interesting AKI mouse model, purifying and quantifying miRNAs by qRT-PCR. They identified 17 upregulated miRNAs with AKI, induced by ischemia-reperfusion damage. The method allows studying the profile of miRNAs in AKI kidneys with a dynamic range, accurate quantification, and high specificity.

show abstract

mentioning

confidence: 99%