Predictable Tissue Shrinkage During Frozen Section Histopathologic Processing for Mohs Micrographic Surgery

Gardner, Erin S.; Sumner, William T.; Cook, Jonathan L.

doi:10.1046/j.1524-4725.2001.01017.x

Cited by 29 publications

(28 citation statements)

References 20 publications

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“…The results are clearly indicating a significant tendency of radiological imaging to underestimate the size of metastasis, the pathological size being significantly higher than the radiological one, despite the fact that the pathological measures were obtained on specimens treated with formalin which normally induce shrinkage of the tumor size by dehydration [24,25]. Figure 1 shows that the difference between pathological and radiological size increases with pathological size (correlation factor = 0.4, p = 0.01).…”

Section: Discussionmentioning

confidence: 95%

Radiological estimation of size in colorectal liver metastases: is it reliable? Comparison with post-resectional measurements

et al. 2010

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Since the efficiency of percutaneous ablation techniques in treating colorectal liver metastases is dependent on tumor size, the aim of this study was to verify the accuracy of computed tomography or magnetic resonance in estimating the maximum diameter of colorectal liver metastases by comparing these findings with those of pathology in a series of patients who underwent liver resection. Radiological and pathological tumor measurements in 39 patients operated for 69 colorectal liver metastasis were recorded. The radiological measurement was performed by magnetic resonance in 40 tumors (23 cases) and by computed tomography in 29 tumors (16 patients). The mean difference between pathological and radiological sizes was 0.4 cm (p < 0.001). Radiological size was smaller than pathological size in 60.9% of tumors, equal in 16% and bigger in 23% of tumors. The results indicated that radiology significantly underestimates the diameter of liver metastasis. To avoid on-site recurrence after percutaneous ablation therapies due to inaccurate radiological measurement, a radiological size of colorectal metastasis up to 2.5 cm should be considered as selection criteria for this treatment.

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Section: Discussionmentioning

confidence: 95%

Radiological estimation of size in colorectal liver metastases: is it reliable? Comparison with post-resectional measurements

et al. 2010

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“…[12][13][14][15][16][17][18][19][20][21] To better understand the relationship between in vivo margins and final histologic margins, causal factors of specimen shrinkage have been evaluated. Factors such as tissue type, tissue location, age of patient, and type of histologic processing can influence the degree of shrinkage.…”

Section: Conclusion and Clinical Relevancementioning

confidence: 99%

“…Factors such as tissue type, tissue location, age of patient, and type of histologic processing can influence the degree of shrinkage. [12][13][14][15] Guidelines to determine the magnitude of surgical margins required for complete excision have been developed on the basis of histologic margins and recurrence rates and known tissue shrinkage patterns for a given neoplasm. [22][23][24] In veterinary medicine, there is a lack of precise guidelines for excision of all tumor types.…”

Section: Conclusion and Clinical Relevancementioning

confidence: 99%

Effect of histologic processing on dimensions of skin samples obtained from cat cadavers

Jeyakumar¹,

Smith²,

Schleis³

et al. 2015

ajvr

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OBJECTIVE To determine changes in dimensions of feline skin samples as a result of histologic processing and to identify factors that contributed to changes in dimensions of skin samples after sample collection. SAMPLE Cadavers of 12 clinically normal cats. PROCEDURES Skin samples were obtained bilaterally from 3 locations (neck, thorax, and tibia) of each cadaver; half of the thoracic samples included underlying muscle. Length, width, and depth were measured at 5 time points (before excision, after excision, after application of ink to mark tissue margins, after fixation in neutral-buffered 10% formalin for 36 hours, and after completion of histologic processing and staining with H&E stain). Measurements obtained after sample collection were compared with measurements obtained before excision. RESULTS At the final time point, tissue samples had decreased in length (mean decrease, 32.40%) and width (mean decrease, 34.21%) and increased in depth (mean increase, 54.95%). Tissue from the tibia had the most shrinkage in length and width and that from the neck had the least shrinkage. Inclusion of underlying muscle on thoracic skin samples did not affect the degree of change in dimensions. CONCLUSIONS AND CLINICAL RELEVANCE In this study, each step during processing from excision to formalin fixation and histologic processing induced changes in tissue dimensions, which were manifested principally as shrinkage in length and width and increase in depth. Most of the changes occured during histologic processing. Inclusion of muscle did not affect thoracic skin shrinkage. Shrinkage should be a consideration when interpreting surgical margins in clinical cases. 945).

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“…A recent study suggests however that clip migration is rare (19). Histopathological excision margins are likely to be underestimated due to tissue shrinkage and electrocautery, one study suggesting that tissue can shrink by up to 10% (20). With a median coronal margin of 15mm the median errors due to tissue shrinkage would be in the order of 1-2mm and, although these might have lead to a small overestimation of the margins required to generate CTV tailored , they are unlikely to affect the conclusions of this study.…”

Section: Discussionmentioning

confidence: 99%

How does knowledge of three-dimensional excision margins following breast conservation surgery impact upon clinical target volume definition for partial-breast radiotherapy?

Kirby

Evans

Nerurkar³

et al. 2010

Radiotherapy and Oncology

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Predictable Tissue Shrinkage During Frozen Section Histopathologic Processing for Mohs Micrographic Surgery

Cited by 29 publications

References 20 publications

Radiological estimation of size in colorectal liver metastases: is it reliable? Comparison with post-resectional measurements

Radiological estimation of size in colorectal liver metastases: is it reliable? Comparison with post-resectional measurements

Effect of histologic processing on dimensions of skin samples obtained from cat cadavers

How does knowledge of three-dimensional excision margins following breast conservation surgery impact upon clinical target volume definition for partial-breast radiotherapy?

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