By tracking the divergence of two initially close trajectories in phase space in an Eulerian approach to forced turbulence, the relation between the maximal Lyapunov exponent λ, and the Reynolds number Re is measured using direct numerical simulations, performed on up to 2048 3 collocation points. The Lyapunov exponent is found to solely depend on the Reynolds number with λ ∝ Re 0.53 and that after a transient period the divergence of trajectories grows at the same rate at all scales. Finally a linear divergence is seen that is dependent on the energy forcing rate. Links are made with other chaotic systems.In Press Physical Review Letters 2018 Using the Eulerian approach, we track the divergence of fluid field trajectories, which initially differ by a small perturbation. We do a model independent analysis, evolving the Navier-Stokes equations for three dimensional homogeneous isotropic turbulence (HIT) using direct numerical simulation (DNS). The Eulerian approach to the study of the chaotic properties of turbulence has received only limited numerical tests prior to this Letter. Amongst approximate models, there have been EDQNM closure approximations [18] and shell model studies [19][20][21]. Amongst exact DNS studies, there have been some in two dimensions [22][23][24] and single runs in three dimensions at comparatively small box sizes [25,26], all more than a decade and a half ago. This Letter tests the theory of Ruelle [27] relating the maximal Lyapunov exponent λ and Re in DNS of HIT in a Eulerian sense. The paper also examines the time history of the divergence and finds a uniform exponential growth rate across all scales at an intermediate time and to show a linear growth for late time in three dimensional HIT. The simulations are also the largest yet for measuring the Eulerian aspects of chaos in HIT for DNS, performed on up to 2048 3 collocation points and reach an integral scale Reynolds number of 6200. This allows a more accurate measurement of the Re dependence of λ.For a chaotic system, an initially small perturbation |δu 0 | should grow according to |δu(t)| ≃ |δu 0 |e λt where t is time. It is theoretically predicted that the Lyapunov exponent should depend on the Reynolds number according to the rule [27,28] The Holder exponent, h, is given by |u(x + r) − u(x)| ∼ V l h , where V is the rms velocity, l the size of the eddy, Re = V L/ν the integral scale Reynolds number, L = (3π/4E) (E(k)/k)dk the integral length scale, E the energy, ν the viscosity, T 0 = L/V the large eddy turnover time, τ = (ν/ǫ) 1/2 the Kolmogorov time scale, and ǫ the dissipation rate. In the Kolmogorov theory, h is predicted to be 1/3 and so α is predicted to be 1/2 [27][28][29].Some of the new results found in this Letter from the Eulerian approach are inaccessible to the Lagrangian approach, such as the linear growth rate of the divergence at late times which has no direct Lagrangian counterpart. The paper also highlights different results from the two approaches. For instance, within the Lagrangian approach, the relation...