Predatory bacteria can reduce Pseudomonas aeruginosa induced corneal perforation and proliferation in a rabbit keratitis model

Romanowski, Eric G.; Stella, Nicholas A.; Brazile, Bryn; Lathrop, Kira L.; Franks, Jonathan; Sigal, Ian A.; Kim, Tami; Elsayed, Mennat; Kadouri, Daniel E.; Shanks, Robert M Q

doi:10.1016/j.jtos.2023.05.002

Cited by 8 publications

(7 citation statements)

References 54 publications

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“…Unlike the tested pathogens, predatory bacteria failed to significantly induce intraocular cytokine levels above that of eyes injected with the PBS vehicle. This is in agreement with prior in vitro and in vivo studies [12, 13, 19, 20, 28, 32, 40–42] and, at least for B. bacteriovorus due to its unusual outer membrane composition and membrane-sheathed flagellum which reduce TLR4 and TLR5 activation [43, 44], which are major mediators of inflammation in bacterial endophthalmitis [45, 46].…”

Section: Discussionsupporting

confidence: 91%

“…Recent studies have evaluated the use of predatory bacteria for treatment of ocular surface infections [17][18][19]. These have demonstrated some efficacy against Escherichia coli [17] in a mouse model and Pseudomonas aeruginosa in rabbit models including the prevention of corneal perforations [19]. However freeze-dried B. bacteriovorus proved to be ineffective in treating Moraxella bovis in a large animal keratoconjunctivitis model [20].…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have evaluated the use of predatory bacteria for treatment of ocular surface infections [17–19]. These have demonstrated some efficacy against Escherichia coli [17] in a mouse model and Pseudomonas aeruginosa in rabbit models including the prevention of corneal perforations [19].…”

Section: Introductionmentioning

confidence: 99%

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Predatory bacteria prevent the proliferation of intraocular Serratia marcescens and fluoroquinolone-resistant Pseudomonas aeruginosa

Romanowski,

Brothers,

Calvario

et al. 2024

Microbiology

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Endogenous endophthalmitis caused by Gram-negative bacteria is an intra-ocular infection that can rapidly progress to irreversible loss of vision. While most endophthalmitis isolates are susceptible to antibiotic therapy, the emergence of resistant bacteria necessitates alternative approaches to combat intraocular bacterial proliferation. In this study the ability of predatory bacteria to limit intraocular growth of Pseudomonas aeruginosa, Serratia marcescens, and Staphylococcus aureus was evaluated in a New Zealand white rabbit endophthalmitis prevention model. Predatory bacteria Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus were able to reduce proliferation of keratitis isolates of P. aeruginosa and to a lesser extent S. marcescens. However, it was not able to significantly reduce the number of intraocular S. aureus, which is not a productive prey for these predatory bacteria, suggesting that the inhibitory effect on P. aeruginosa and S. marcescens requires active predation rather than an antimicrobial immune response. Similarly, UV-inactivated B. bacteriovorus were unable to prevent proliferation of P. aeruginosa. Together, these data indicate in vivo inhibition of Gram-negative bacteria proliferation within the intra-ocular environment by predatory bacteria.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Predatory bacteria prevent the proliferation of intraocular Serratia marcescens and fluoroquinolone-resistant Pseudomonas aeruginosa

Romanowski,

Brothers,

Calvario

et al. 2024

Microbiology

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“…Nevertheless, the reduced severity observed in the clinical data from this study were consistent with a recent paper using a rabbit corneal infection model that demonstrated reduced corneal perforation and bacterial proliferation of a lasR deletion mutant of strain PA14 compared to an isogenic wild type suggesting that LasR promotes keratitis severity (Romanowski et al, 2023). Studies with mice show mixed results with strain PA01 with lasR deletion mutations.…”

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confidence: 91%

A rise in the frequency of lasR mutant Pseudomonas aeruginosa among keratitis isolates between 1993 and 2021

Shanks,

Atta,

Stella

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionPseudomonas aeruginosa causes vision threatening keratitis. The LasR transcription factor regulates virulence factors in response to the quorum sensing molecule N-3-oxo-dodecanoyl-L-homoserine lactone. P. aeruginosa isolates with lasR mutations are characterized by an iridescent high sheen phenotype caused by a build-up of 2-heptyl-4-quinolone. A previous study demonstrated 22% (n=101) of P. aeruginosa keratitis isolates from India between 2010 and 2016 were sheen positive lasR mutants, and the sheen phenotype correlated with worse clinical outcomes for patients. In this study, a longitudinal collection of P. aeruginosa keratitis isolates from Eastern North America were screened for lasR mutations by the sheen phenotype and sequencing of the lasR gene.MethodsKeratitis isolates (n=399) were classified by sheen phenotype. The lasR gene was cloned from a subset of isolates, sequenced, and tested for loss of function or dominant-negative status based on an azocasein protease assay. A retrospective chart review compared outcomes of keratitis patients infected by sheen positive and negative isolates.ResultsA significant increase in sheen positive isolates was observed between 1993 and 2021. Extracellular protease activity was reduced among the sheen positive isolates and a defined lasR mutant. Cloned lasR alleles from the sheen positive isolates were loss of function or dominant negative and differed in sequence from previously reported ocular lasR mutant alleles. Retrospective analysis of patient information suggested significantly better visual outcomes for patients infected by sheen positive isolates.DiscussionThese results indicate an increase in lasR mutations among keratitis isolates in the United States and suggest that endemic lasR mutants can cause keratitis.

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“…Finally, the diversity indices (Chao1, Coverage, Simpson, and Shannon indices) of soil bacteria were calculated by IIME 2 software (Puga et al, 2015;Kiran and Prasad, 2019). Fisher's exact test was carried out to detect species with abundance difference between groups, and hypothesis testing was carried out to evaluate the significance of observed differences (Romanowski et al, 2023). Bacterial diversity analysis was performed under the soil Cd content 4 mg•kg −1 (H0T, H3T, H3B, and H3J).…”

Section: Determination Of Soil Bacterial Community Diversitymentioning

confidence: 99%

Differences in soil bacterial community structure during the remediation of Cd-polluted cotton fields by biochar and biofertilizer in Xinjiang, China

Zhu,

An,

Anwar

et al. 2024

Front. Microbiol.

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IntroductionHeavy metal pollution is a major worldwide environmental problem. Many remediation techniques have been developed, these techniques have different performance in different environments.MethodsIn this study, soil sampling was conducted in multiple cotton fields in Xinjiang, China, and found that cadmium (Cd) was the most abundant soil heavy metal. Then, to find the most suitable technique for the remediation of Cd pollution in cotton fields, a two-year study was conducted to explore the effects of cotton straw-derived biochar (BC, 3%) and Bacillus-based biofertilizer (BF, 1.5%) on cotton Cd uptake and transport and soil microbial community structure under Cd exposure conditions (soil Cd contents: 1, 2, and 4 mg·kg−1).ResultsThe results showed that the bioaccumulation coefficients (Cd content of cotton organs / soil available Cd content) of cotton roots, stems, leaves, and buds/bolls reduced by 15.93%, 14.41%, 23.53%, and 20.68%, respectively after the application of BC, and reduced by 16.83%, 17.15%, 22.21%, and 26.25%, respectively after the application of BF, compared with the control (no BC and BF). Besides, the application of BC and BF reduced the transport of Cd from soil to root system, and enhanced the diversity of soil bacterial communities (dominant species: Alphaproteobacteria and Actinobacteria) and the metabolic functions related to amino acid synthesis. It was worth noting that the differential species for BF group vs BC group including Alphaproteobacteria, Gemmatimonadetes, Bacilli, and Vicinamibacteria were associated with the enrichment and transport of Cd, especially the transport of Cd from cotton roots to stems.DiscussionTherefore, the application of BC and BF changed the soil bacterial diversity in Cd-polluted cotton field, and then promoted the transport of Cd in cotton, ultimately improving soil quality. This study will provide a reference for the selection of soil heavy metal pollution remediation techniques in Xinjiang, China.

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Predatory bacteria can reduce Pseudomonas aeruginosa induced corneal perforation and proliferation in a rabbit keratitis model

Cited by 8 publications

References 54 publications

Predatory bacteria prevent the proliferation of intraocular Serratia marcescens and fluoroquinolone-resistant Pseudomonas aeruginosa

Predatory bacteria prevent the proliferation of intraocular Serratia marcescens and fluoroquinolone-resistant Pseudomonas aeruginosa

A rise in the frequency of lasR mutant Pseudomonas aeruginosa among keratitis isolates between 1993 and 2021

Differences in soil bacterial community structure during the remediation of Cd-polluted cotton fields by biochar and biofertilizer in Xinjiang, China

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