Precursor feeding enhances L-Dopa production in hairy root culture of Hybanthus enneaspermus (L.) F.Muell

Sathish, Selvam; Vasudevan, Venkatachalam; Karthik, Sivabalan; Pavan, Gadamchetty; Siva, Ramamoorthy; Manickavasagam, Markandan

doi:10.1007/s11756-022-01308-z

Cited by 4 publications

(1 citation statement)

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“…This is similar to the ndings of Zhang et al (2020), who noted that higher production of wilforgine and wilforine was achieved at 35 days (exponential growth phase) of Tripterygium wilfordii hairy roots. The higher yield of L-Dopa was found at the 40th day in the growth curve of Hybanthus enneaspermus hairy root (Sathish et al 2023). In another study, reported that the highest production of plumbagin in Plumbago zeylanica hairy root cultures was achieved at day 25.…”

Section: Discussionmentioning

confidence: 92%

Elicitation boosts withaferin A and withanolide A production in Withania somnifera (L.) Dunal hairy root cultures

Mahendran,

Vimolmangkang

2024

Preprint

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Withania somnifera (L.) Dunal, an Ayurvedic herb rich in withanosides, has anti-cancer and health-rejuvenating properties. These bioactive chemicals, primarily found in the roots, are of significant interest for production in hairy root cultures. In this study, researchers investigated a hairy root line produced by Rhizobium rhizogenes (ATCC 43057), varying cell density, infection period, and acetosyringone concentrations. The most successful root induction frequency (31.66% ± 2.98%) occurred at a bacterial cell density of OD600 = 0.8 combined with a 20-min infection period and 200 µM acetosyringone. After 25 days, the hairy root culture achieved its highest biomass (5.20 ± 0.15 g/50 mL flask fresh weight (FW)) in MS liquid medium with 3% sucrose. At 30 and 25 days, the greatest amounts of withanolide A (0.824 ± 0.01 mg/g DW) and withaferin A (1.292 ± 0.02 mg/g DW) were observed. Hairy roots were further treated with methyl jasmonate (MJ) and salicylic acid (SA) at various concentrations (25, 50, and 100 µM). Notably, the maximum content of withanolide A (2.90 ± 0.08 mg/g DW, 3.86-fold) and withaferin A (3.40 ± 0.01 mg/g DW, 2.61-fold) was obtained in the hairy roots treated with MJ at 100 µM. After 48 h, the content of withanolide A reached 3.16 ± 0.03 mg/g DW (6.72-fold), and withaferin A peaked at 4.32 ± 0.05 mg/g DW (4.59-fold) when treated with 50 µM SA + 100 µM MJ. This study demonstrated that W. somnifera hairy root culture enhances large-scale production of withanolide A and withaferin A.

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Section: Discussionmentioning

confidence: 92%