Precursor-directed biosynthesis of fluorinated iturin A in Bacillus spp.

Moran, Stephen; Dilip, K.; Clark, Benjamin R.; Murphy, Cormac D.

doi:10.1039/b816345f

Cited by 35 publications

(33 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The PCR analysis further confirms the presence of ItuD and SrfC lipopeptide genes in isolate YSPMK11 which were responsible for the antifungal activities. Iturin A has reported to have strong haemolytic activity which kills wide range of fungal phytopathogens, whereas surfactin help in swarming, cell spreading, and biofilm production which favours the bacteria to colonize the root cells of plants (Moran et al 2009;Yuan et al 2011). In this study, we also found the potential antifungal compounds produced by B. pumilus.…”

Section: Discussionsupporting

confidence: 59%

Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum

2017

View full text Add to dashboard Cite

A study was executed in a direction to attenuate Sclerotinia stalk rot (SSR) disease through biocontrol agent and also to enhance crop productivity. Culture filtrate of bacterial strain YSPMK11 inhibited growth of Sclerotinia sclerotiorum in vitro which also exhibited higher plant growth promoting attributes. Interaction studies revealed maximum (81.50%) growth inhibition at 35°C and pH 7.0 after 72 h incubation period with 15% culture filtrate. Based upon 16S rRNA gene sequence strain, YSPMK11 was identified as Bacillus pumilus. Furthermore, the genome of this isolate was searched for antimicrobial lipopeptide, i.e., ItuD and SrfC genes. The PCR amplification results showed the presence of both these lipopeptide genes in isolate YSPMK11. Iturin A as antifungal compound was identified as major components of fraction through GC/MS. In field experiments, the application of strain YSPMK11 cell suspension (10 8 CFU/ml) suppressed disease severity by 93% and increased curd yield by 36% which was more that of commercially used fungicide in farmer practices under mid-hills of Himachal Pradesh.Conclusively, our study is first to demonstrate the effect of B. pumilus strain YSPMK11 in suppression of SSR under field conditions and would be employed as an efficient biocontrol agent to replace commercial fungicides in cauliflower cropping system. In addition, the presence of both lipopeptide genes (ItuD and SrfC) and iturin A in this isolate makes him potent strain for biological control application in agriculture.

show abstract

Section: Discussionsupporting

confidence: 59%

Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum

2017

View full text Add to dashboard Cite

show abstract

“…The flow rate and the temperature of the column was optimized by using standard Iturin A >90% (Sigma Aldrich, Canada) to allow for appropriate separation of iturin A from any impurities. Standard curve was made by running different concentrations of standard iturin A (10,20,30,40,50,60,70,80, 90 and 100 ppm). The optimized flow rate was 1.0 mL/min and temperature of column compartment was set at 25°C with injection volume of 10 μL.…”

Section: Quantification Of Iturin a By Liquid Chromatographymentioning

confidence: 99%

Genetic, physiological and biochemical characterization of Bacillus sp . strain RMB7 exhibiting plant growth promoting and broad spectrum antifungal activities

et al. 2014

View full text Add to dashboard Cite

Background: Plant growth promoting rhizobacteria (PGPR) are functionally diverse group of bacteria having immense potential as biofertilizers and biopesticides. Depending upon their function, they may serve as partial replacements for chemical fertilizer or pesticides as an eco-friendly and cost-effective alternatives as compared to their synthetic counterparts. Therefore, isolation, characterization and practical evaluation of PGPRs having the aforementioned multifaceted beneficial characteristics, are essentially required. This study describes the detailed polyphasic characterization of Bacillus sp. strain RMB7 having profound broad spectrum antifungal activity and plant growth promoting potential. Results: Based on 16S rRNA gene sequencing, strain RMB7 was identified as Bacillus specie. This strain exhibited the production of 8 mg. L −1 of indole-3-acetic acid (IAA) in tryptophan-supplemented medium. It was able to solubilize 50.6 mg. L −1 tri-calcium phosphate, reduced 601ηmol acetylene h −1 /vial and inhibited >70% growth of nine fungal phytopathogens tested in vitro. Under natural pathogen pressure, inoculation with strain RMB7 and RMB7-supernatant conferred resistance by arugula plant against Pythium irregulare with a concurrent growth improvement over non-inoculated plants. The T-RFLP analysis based on 16S rRNA gene showed that inoculation with RMB7 or its supernatant have a major impact on the indigenous rhizosphere bacterial population. Mass spectrometric analysis revealed the production of lipopeptide surfactins as well as iturin A presence in crude extract of RMB7. PCR-amplification further confirmed the presence of genes involved in the biosynthesis of these two bioactive lipopeptide compounds.

show abstract

“…The growth inhibitory effects caused by NCAAs, which have been demonstrated in different species including bacteria [65,66,67], yeasts [68], insects [69,70], and mammals [71], comprise one major challenge encountered during genetic code modification. The inhibitory effects of NCAAs are mainly attributable to two aspects.…”

Section: Challenges Of Genetic Code Engineeringmentioning

confidence: 99%

Efforts and Challenges in Engineering the Genetic Code

Lin

Chan

2017

Life

View full text Add to dashboard Cite

This year marks the 48th anniversary of Francis Crick’s seminal work on the origin of the genetic code, in which he first proposed the “frozen accident” hypothesis to describe evolutionary selection against changes to the genetic code that cause devastating global proteome modification. However, numerous efforts have demonstrated the viability of both natural and artificial genetic code variations. Recent advances in genetic engineering allow the creation of synthetic organisms that incorporate noncanonical, or even unnatural, amino acids into the proteome. Currently, successful genetic code engineering is mainly achieved by creating orthogonal aminoacyl-tRNA/synthetase pairs to repurpose stop and rare codons or to induce quadruplet codons. In this review, we summarize the current progress in genetic code engineering and discuss the challenges, current understanding, and future perspectives regarding genetic code modification.

show abstract

Precursor-directed biosynthesis of fluorinated iturin A in Bacillus spp.

Cited by 35 publications

References 17 publications

Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum

Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum

Genetic, physiological and biochemical characterization of Bacillus sp . strain RMB7 exhibiting plant growth promoting and broad spectrum antifungal activities

Efforts and Challenges in Engineering the Genetic Code

Contact Info

Product

Resources

About