Precursor Cells to Erythroblasts and to Small Lymphocytes of the Bone Marrow

Rosse, Cornelius

doi:10.1002/9780470719961.ch5

Cited by 10 publications

(1 citation statement)

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“…The comparable blast cell activity and the nearly identical slopes of the labelling curves of bone marrow lymphocytes during injections of 3H-TdR demonstrates that the production of lymphocytes and the sojourn of cells in the marrow are comparable in Splx and Sh-Splx normal mice. Furthermore, the findings in Splx nude mice of 'normal' kinetics of the remaining bone marrow lymphocytes and unchanged blood lymphocyte levels indicate that the increased numbers of newly-formed lymphocytes in the peripheral tissues af Splx mice may be maintained in part by an increased release from the bone marrow of 'in excess' produced lymphocytes (Rosse 1973), which are allowed to survive outside the bone marrow as a compensation for a loss of thymusderivedpredominantly long-lived (Osmond 1972) -lymphocytes.…”

Section: Discussionmentioning

confidence: 96%

Kinetics of Small Lymphocytes in Normal and Nude Mice after Splenectomy

Hougen

Hansen

Jensen

et al. 1977

Scandinavian Journal of Haematology

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Autoradiography and various quantitations on lymphoid tissues have been used to evaluate the kinetics of small lymphocytes in normal (+/nu or +/+) and congenitally athymic nude (nu/nu) NMRI mice 1 month after splenectomy or sham‐splenectomy. The results indicate that splenectomy causes depressed thymic activity and diminished numbers of T lymphocytes in peripheral lymphoid tissues. The total number of cells in these tissues as well as the blast cell activity, were within normal limits. Bone marrow lymphocyte numbers and kinetics as well as blood lymphocyte levels in splenectomized and sham‐splenectomized normal animals were comparable. Blood lymphocyte numbers were at normal levels in splenectomized nude mice, in spite of reduced numbers of bone marrow and thoracic duct lymphocytes. It is suggested that increased number of newly‐formed lymphocytes, found in lymph nodes and blood of splenectomized mice, are released from the lympho‐myeloid organs in compensation for the loss of long‐lived, thymus‐derived cells.

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Section: Discussionmentioning

confidence: 96%

Kinetics of Small Lymphocytes in Normal and Nude Mice after Splenectomy

Hougen

Hansen

Jensen

et al. 1977

Scandinavian Journal of Haematology

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A cytochemical and radioautographic analysis of erythropoiesis at the ultrastructural level

Rosse

Trotter

1974

Am. J. Anat.

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The morphogenetic events of erythroid differentiation in guinea pig bone marrow were studied by electron microscopy. The diaminobenzidine (DAB ) reaction and radioautographic detection of Fe55 incorporation permitted a more objective identification of erythroid cells than was possible in previous studies. With the combined use of these two methods morphological events of cell maturation could be correlated reliably with cytoplasmic hemoglobin content, thus providing a basis for the description of various maturation stages.The oxidation of DAB by hemoglobin coupled with OsOl treatment results in a generalized increase in cytoplasmic electron opacity proportional to the amount of hemoglobin present. Measurements of cytoplasmic density were carried out on electron microscopic negatives of DAB-reacted specimens that were not stained otherwise. Only those cells were regarded as erythroblasts in which the cytoplasm was at least twice as dense as in recognizable, differentiated, non-erythroid cells, using reticular cell cytoplasm in each specimen as the base line for the comparisons.In bone marrow cells, iron incorporation occurred overwhelmingly for hemoglobin synthesis. In normal marrow, blast cells which incorporated iron but did not contain hemoglobin in detectable amounts were defined as Stage I erythroid cells. They were morphologically of the same type as erythroblasts with the minimum amount of detectable hemoglobin (Stage 11). Cells in maturation stages of progressively increasing hemoglobin content were assigned to Stages I11 to V. Stage I11 erythroblasts correspond morphologically to proerythroblasts of light microscopic preparations. The DAB reaction in them was obvious without densitometric measurements but remained mottled.The morphological characteristics of each of the maturation stages are described with particular emphasis on the earliest erythroid cells, since the methods employed permit their identification with greater degrees of discrimination and objectivity than is possible with the light microscope or with conventional methods of electron microscopy.The DAB reaction was positive in the heterochromatin in Stages I11 and IV but it was negative in the pyknotic chromatin of erythroblasts in Stage V of maturation. The predominantly extended chromatin and also the large and complex nucleoli of earlier cells began to condense during Stage IV. Silver grains due to Fej5 disintegration were observed over the nuclei of erythroblasts in all maturational stages.The perinuclear cisterna was DAB-negative and it became wider as maturation progressed. It was bridged in places by DAB-negative material connecting the cytoplasm to euchromatic areas of the nucleus. DAB-positive substance was not seen in nuclear pores at any stage of maturation. At the time of extrusion, nuclei did not contain DAB-positive material but were occasionally labeled with Fe5j.

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Progenitor cells of erythroblasts: An in vitro investigation of erythropoietin‐responsive cells of guinea pig bone marrow

Rosse

Beaufait

1978

Anat. Rec.

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The experiments were designed to test whether or not erythroblast progenitor cell function could be demonstrated in a morphological cell type designated as "transitional cells." Two cell fractions, were obtained from the bone marrow of normal and polycythemic guinea pigs. One fraction (F1) was enriched in transitional cells and contained few, if any, other cell types which could be considered as candidates for erythropoietin responsive cells (ERC). The other fraction (F2) contained undifferentiated blast cells as well as transitional cells. The effect of human urinary erythropoiesis stimulating factors (ESF) on heme synthesis was compared in these two fractions by measuring 59Fe incorporation into heme. ESF was more effective in stimulating heme synthesis in guinea pig bone marrow cells than homologous sera obtained from anemic or hypoxic animals. The majority of ERC sedimented in F2, but the stimulation index was comparable in the two fractions. It was confirmed by radioautography that the ESF response in F1 was due to the generation of proerythroblasts and basophilic erythroblasts that incorporated 55Fe. The generation of these cells in F1 was dependent on the addition of ESF to the cultures, whereas 55Fe-labeled erythroblasts were recovered from cultured of F2 not supplemented with ESF. ESF induced a proportion of transitional cells to incorporate 55Fe in both F1 and F2. Transitional cells were the only cell type in which heme synthesis was dependent on ESF. in other cells of clearly nonerythroid morphology (mononuclear phagocytes and reticular cells), 55Fe incorporation occurred independent of ESF. Although the fractionation procedure employed is unsuitable for the separation of ERC from bone marrow, it permitted the enrichment of transitional cells, a cell type defined by morphology. Radioautography with 55Fe identified a proportion of these cells as ERC in both F1 and F2 fractions of bone marrow obtained from normal and polycythemic guinea pigs. Although there may be other cell types in F2 capable of responding to ESF, the present studies show that some transitional cells function as progenitors of erythroblasts because they respond to ESF by initiation of heme synthesis and by transformation into the earliest recognizable erythroid cells.

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Precursor Cells to Erythroblasts and to Small Lymphocytes of the Bone Marrow

Cited by 10 publications

References 20 publications

Kinetics of Small Lymphocytes in Normal and Nude Mice after Splenectomy

Kinetics of Small Lymphocytes in Normal and Nude Mice after Splenectomy

A cytochemical and radioautographic analysis of erythropoiesis at the ultrastructural level

Progenitor cells of erythroblasts: An in vitro investigation of erythropoietin‐responsive cells of guinea pig bone marrow

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