Preconditioning with far-infrared irradiation enhances proliferation, cell survival, and migration of rat bone marrow-derived stem cells via CXCR4-ERK pathways

Abstract

Far-infrared radiation (FIR) has been shown to exert positive effects on the cardiovascular system. However, the biological effects of FIR on bone marrow-derived stem cells (BMSCs) are not understood. In the present study, BMSCs were isolated from rat femur bone marrow and cultured in vitro. To investigate the effects of an FIR generator with an energy flux of 0.13 mW/cm2 on rat BMSCs, survival of BMSCs was measured by crystal violet staining, and cell proliferation was additionally measured using Ez-Cytox cell viability, EdU, and Brd U assays. FIR preconditioning was found to significantly increase BMSC proliferation and survival against H2O2. The scratch and transwell migration assays showed that FIR preconditioning resulted in an increase in BMSC migration. qRT-PCR and Western blot analyses demonstrated that FIR upregulated Nanog, Sox2, c-Kit, Nkx2.5, and CXCR4 at both the mRNA and protein levels. Consistent with these observations, PD98059 (an ERK inhibitor) and AMD3100 (a CXCR4 inhibitor) prevented the activation of CXCR4/ERK and blocked the cell proliferation and migration induced by FIR. Overall, these findings provide the first evidence that FIR confers a real and significant benefit on the preconditioning of BMSCs, and might lead to novel strategies for improving BMSC therapy for cardiac ischemia.

FAM3C‐YY1 axis is essential for TGFβ‐promoted proliferation and migration of human breast cancer MDA‐MB‐231 cells via the activation of HSF1

“…The method for transwell migration assay was detailed elsewhere. 6,31 In brief, MDA-MB-231 cells treated with different conditions for 24 hours were seeded into the upper transwell chambers (Corning, Lot#3422) containing DMEM medium without FBS (30 000 cells seeded per chamber). The chambers were inserted into each well of 24-well plates containing 600 μL DMEM medium with 10% FBS.…”

Instability of Nucleic Acids in Airborne Microorganisms under Far Infrared Radiation

“…FIR radiation could be applied directly into patient's mouth to diminish large amounts of RNA viruses on surface of cells, which could shut off the circulation of them and improve lung activation by absorbing fresh and clean air. As FIR radiation could improve cell proliferation (Jeong et al, 2017), it might improve highly injured lung to recover. Further, as FIR radiation could penetrate about 4 cm tissue (Mero et al, 2015), it would be very helpful to apply FIR radiation to RNA virus infected patients' skin to improve cell activity and inhibit RNA virus in blood.…”

Substance P enhances the local activation of NK1R-expressing c-Kit+ cardiac progenitor cells in right atrium of ischemia/reperfusion-injured heart

“…To determine the priming effects of SP on c-Kit + CPC migration, a cell migration assay was performed using 0.8 mm pore size, and 24 well transwell migration chambers coated with Type IV collagen (10 mg/ml) as previously described [16].…”

“…StepOnePlus real-time PCR system (Applied Biosystems). The relative gene expression from all data were obtained using the ΔCt method with normalization versus RPL-32 as previously described [16,19]. The primers used were: NK 1 R (Forward-TACACTGTGGGGCCAGTGAGATC, Reverse-GGTACACACAACCACGATCATCA); c-KIT (Forward-AGACGTACAGATCCAGAATG, Reverse-TGCTCTTTGCTGTTACCTT); NANOG (Forward-CTCTCTACCATTCTGAACCTGAGC, Reverse-TCAGGCCGTTGCTAGTCTTC); OCT4 (Forward-GCCCCCATTTCACCACACT, Reverse-CCAGAGCAGTGACAGGAACA); SOX2…”

“…The c-Kit + CPCs were xed with 4% paraformaldehyde. They were stained with standard IFS methods as previous described [2,16,19]. After nuclear DAPI or Hoechst 33342 staining, immunostained confocal images were acquired using an inverted Zeiss Axio Observer Z1 microscope with 405, 458, 488, 514, 561, and 633 nm laser lines.…”