Preconditioning with Cations Increases the Attachment of Anoxybacillus flavithermus and Geobacillus Species to Stainless Steel

Somerton, Ben; Flint, Steve; Palmer, John W.; Brooks, J.; Lindsay, D.

doi:10.1128/aem.00462-13

Cited by 19 publications

(7 citation statements)

References 25 publications

(39 reference statements)

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“…Geobacillus is very well reported in the literature for biofilm and spore formation [95,96]. Anthranilate phosphoribosyltransferase (EC 2.4.2.18) [97] and 5 -nucleotidase (EC 3.1.3.5), reported to have an impact on virulence and biofilm formation, were found in our Geobacil-lus sp.…”

Section: Genetic Determinants For Biofilm Formation and Regulationsupporting

confidence: 78%

Genome Annotation of Poly(lactic acid) Degrading Pseudomonas aeruginosa, Sphingobacterium sp. and Geobacillus sp.

Satti

Castro‐Aguirre

Shah

et al. 2021

IJMS

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Pseudomonas aeruginosa and Sphingobacterium sp. are well known for their ability to decontaminate many environmental pollutants while Geobacillus sp. have been exploited for their thermostable enzymes. This study reports the annotation of genomes of P. aeruginosa S3, Sphingobacterium S2 and Geobacillus EC-3 that were isolated from compost, based on their ability to degrade poly(lactic acid), PLA. Draft genomes of the strains were assembled from Illumina reads, annotated and viewed with the aim of gaining insight into the genetic elements involved in degradation of PLA. The draft genome of Sphinogobacterium strain S2 (435 contigs) was estimated at 5,604,691 bp and the draft genome of P. aeruginosa strain S3 (303 contigs) was estimated at 6,631,638 bp. The draft genome of the thermophile Geobacillus strain EC-3 (111 contigs) was estimated at 3,397,712 bp. A total of 5385 (60% with annotation), 6437 (80% with annotation) and 3790 (74% with annotation) protein-coding genes were predicted for strains S2, S3 and EC-3, respectively. Catabolic genes for the biodegradation of xenobiotics, aromatic compounds and lactic acid as well as the genes attributable to the establishment and regulation of biofilm were identified in all three draft genomes. Our results reveal essential genetic elements that facilitate PLA metabolism at mesophilic and thermophilic temperatures in these three isolates.

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Section: Genetic Determinants For Biofilm Formation and Regulationsupporting

confidence: 78%

Genome Annotation of Poly(lactic acid) Degrading Pseudomonas aeruginosa, Sphingobacterium sp. and Geobacillus sp.

Satti

Castro‐Aguirre

Shah

et al. 2021

IJMS

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“…Also, it was hypothesized that low Ca 2+ or Mg 2+ concentrations have the potential to inhibit biofilm formation by some A. flavithermus and Geobacillus spp. strains during the processing of milk formulations (Somerton et al, 2013 ). Another recent study has showed that biofilm formation decreased with increasing concentration of Mg 2+ in Enterobacter cloacae (Zhou et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Magnesium ions mitigate biofilm formation of Bacillus species via downregulation of matrix genes expression

2015

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The objective of this study was to investigate the effect of Mg2+ ions on biofilm formation by Bacillus species, which are considered as problematic microorganisms in the food industry. We found that magnesium ions are capable to inhibit significantly biofilm formation of Bacillus species at 50 mM concentration and higher. We further report that Mg2+ ions don't inhibit bacterial growth at elevated concentrations; hence, the mode of action of Mg2+ ions is apparently specific to inhibition of biofilm formation. Biofilm formation depends on the synthesis of extracellular matrix, whose production in Bacillus subtilis is specified by two major operons: the epsA-O and tapA operons. We analyzed the effect of Mg2+ ions on matrix gene expression using transcriptional fusions of the promoters for eps and tapA to the gene encoding β galactosidase. The expression of the two matrix operons was reduced drastically in response to Mg2+ ions suggesting about their inhibitory effect on expression of the matrix genes in B. subtilis. Since the matrix gene expression is tightly controlled by Spo0A dependent pathway, we conclude that Mg2+ ions could affect the signal transduction for biofilm formation through this pathway.

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“…Bellona and Drewes () reported that positively charged ions such as sodium, calcium, magnesium, and cationic surfactants can bind and neutralize negatively charged surfaces and enhance bacterial attachment to a filter membrane. Somerton and others () found that changes in salt concentration (calcium and magnesium) influenced the attachment of Geobacillus and Anoxybacillus species to SS surfaces. In our study, 1% RWPC80 with additional lactose and minerals (calcium and magnesium) did increase the magnitude of biofilm formation by B. licheniformis isolates by 1‐ to‐ 2‐fold except for one strain (D55C04) which was unable to form biofilm.…”

Section: Resultsmentioning

confidence: 99%

“…All these factors will vary during the process and are likely to have an influence on biofilm development. For example, Somerton and others () found that changes in salt concentration influenced the attachment of Geobacillus and Anoxybacillus species to SS surfaces. Understanding the source of contamination and development of B. licheniformis biofilms will be important in developing methods to control or prevent biofilm formation and the contamination of WPC.…”

Section: Introductionmentioning

confidence: 99%

The Potential Source of B. licheniformis Contamination During Whey Protein Concentrate 80 Manufacture

Zain

Bennett

Flint

2017

Journal of Food Science

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The objective of this study was to determine the possible source of predominant Bacillus licheniformis contamination in a whey protein concentrate (WPC) 80 manufacturing plant. Traditionally, microbial contaminants of WPC were believed to grow on the membrane surfaces of the ultrafiltration plant as this represents the largest surface area in the plant. Changes from hot to cold ultrafiltration have reduced the growth potential for bacteria on the membrane surfaces. Our recent studies of WPCs have shown the predominant microflora B. licheniformis would not grow in the membrane plant because of the low temperature (10 °C) and must be growing elsewhere. Contamination of dairy products is mostly due to bacteria being released from biofilm in the processing plant rather from the farm itself. Three different reconstituted WPC media at 1%, 5%, and 20% were used for biofilm growth and our results showed that B. licheniformis formed the best biofilm at 1% (low solids). Further investigations were done using 3 different media; tryptic soy broth, 1% reconstituted WPC80, and 1% reconstituted WPC80 enriched with lactose and minerals to examine biofilm growth of B. licheniformis on stainless steel. Thirty-three B. licheniformis isolates varied in their ability to form biofilm on stainless steel with stronger biofilm in the presence of minerals. The source of biofilms of thermo-resistant bacteria such as B. licheniformis is believed to be before the ultrafiltration zone represented by the 1% WPC with lactose and minerals where the whey protein concentration is about 0.6%.

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Preconditioning with Cations Increases the Attachment of Anoxybacillus flavithermus and Geobacillus Species to Stainless Steel

Cited by 19 publications

References 25 publications

Genome Annotation of Poly(lactic acid) Degrading Pseudomonas aeruginosa, Sphingobacterium sp. and Geobacillus sp.

Genome Annotation of Poly(lactic acid) Degrading Pseudomonas aeruginosa, Sphingobacterium sp. and Geobacillus sp.

Magnesium ions mitigate biofilm formation of Bacillus species via downregulation of matrix genes expression

The Potential Source of B. licheniformis Contamination During Whey Protein Concentrate 80 Manufacture

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