Preclinical safety evaluation of the aqueous extract from Mangifera indica Linn. (Anacardiaceae): genotoxic, clastogenic and cytotoxic assessment in experimental models of genotoxicity in rats to predict potential human risks

Boas, Gustavo Roberto Villas; Lemos, João Marcos Rodrigues; Oliveira, Matheus William de; Santos, Rafael Claudino dos; Silveira, Ana Paula Stefanello da; Bacha, Flávia Barbieri; Ito, Caren Naomi Aguero; Cornelius, Ediane Bortolotte; Lima, Fernanda Brioli; Rodrigues, Andrea Marisa Sachilarid; Costa, Nathália Belmal; Bittencourt, Felipe Francisco; Lima, Fernando Freitas de; Paes, Marina Meirelles; Gubert, Priscila; Oesterreich, Silvia Aparecida

doi:10.1016/j.jep.2019.112086

Cited by 12 publications

(10 citation statements)

References 53 publications

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“…The lowest dose (ZAad 1 ) triggered a lower frequency of PCEMNs than the negative control (p > 0.05), blank zein nanoparticles (p > 0.05), ZAad 2 (p > 0.05) and DXR (p < 0.001). DXR acts through the topoisomerase II inhibition, causing evident genotoxicity by damaging the DNA, used as positive control in micronucleus assays [ [64] , [65] , [66] ].…”

Section: Resultsmentioning

confidence: 99%

Toxicological and genotoxic evaluation of anacardic acid loaded-zein nanoparticles in mice

et al. 2020

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Section: Resultsmentioning

confidence: 99%

Toxicological and genotoxic evaluation of anacardic acid loaded-zein nanoparticles in mice

et al. 2020

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“…In addition to genotoxicity, micronucleus test allowed detecting the extract's cytotoxicity, since there was significant PCE:NCE ratio reduction in the animals treated with the aqueous extract in compared to the negative group. The research group in charge of the current study had already shown the cytotoxic potential of M. indica leaves, which was proven by assessing their aqueous extract -at lower concentrations (15mg/mL and 30 mg/mL) than that used in the present study -through the Allium cepa system (LIMA et al, 2016) Studies carried out by other authors with aqueous M. indica leaf extract based on methodologies similar the herein adopted one did not detect the genotoxic and cytotoxic potential of this extract (REDDEMAN et al, 2019;VILLAS BOAS et al, 2019) Therefore, changes in these abiotic and biotic factors can explain differences in biological effects observed in previous research.…”

Section: Discussionmentioning

confidence: 51%

“…The wide array of applications and use in popular medicine of M. indica leaf extract highlight the importance of studies regarding its cytotoxic and/or genotoxic potential. Previous studies did not detect any genotoxic activity in aqueous extract from M. indica leaves (LIMA et al, 2016;REDDEMAN et al, 2019;VILLAS BOAS et al, 2019). Other studies reported anticarcinogenic activity in aqueous extract from M. indica leaves (BONTEMPO and ORSOLIN, 2016).…”

Section: Introductionmentioning

confidence: 92%

“…Mangifera indica L., popularly known as mango tree, is a large tree belonging to family Anacardiaceae, native to Asia, and widely grown in tropical and subtropical regions worldwide. The species' bark, roots and leaves are used in popular medicine; its leaves are used to treat diseases such as pain, diarrhea, dysentery, fever, cough, throat affections inflammation, diabetes and burns among others (SEVERI et al, 2009;YAKUBU and SALIMON, 2015;VILLAS BOAS et al, 2019). Studies about the chemical components of M. indica date back to the 20th century and the scientific interest in its biological properties has grown in recent years.…”

Section: Introductionmentioning

confidence: 99%

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Mangifera indica L.: Assessing the cytogenotoxicity of aqueous extract through micronucleus and comet assays

Rocha

Souza

Silva

et al. 2022

CeN

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Mangifera indica L., known as mango tree, is a species belonging to family Anacardiaceae, widely used in popular medicine whose leaves are used to treat several diseases. Although several studies did not detect the cytogenotoxic activity of M. indica leaf extract, other authors have observed these feature and highlighted the importance of further research about the toxic potential of M. indica leaf extract. The aim of the current research is to assess the cytogenotoxic potential of the aqueous extract of M. indica leaves through comet DNA assay and micronucleus test. It was possible observing significant increase in the number of micronucleated polychromatic erythrocytes (MNPCE) in mice subjected to the treatment with the extract, in addition to significant increase in DNA damage index in comparison to the negative control group. The extract also presented cytotoxic effect caused by significant reduction in the polychromatic:normochromatic erythrocyte (PCE/NCE) ratio in comparison to the negative control group. The extract of M. indica leaves is cytogenotoxic under the conditions used in the current research; this outcome highlighted the importance of comparative studies applied to the several used processing methods, as well as to cultivation conditions, to certify the safety in the popular use of medicinal plants.

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“…It also inhibits, in a non-competitively way, the a-amylase enzyme and the substrateenzyme complex, causing a decrease in starch degradation. In addition, some studies have shown that mango leaves do not have a genotoxic, cytotoxic or clastogenic effect making them suitable for human consumption (Zhang et al, 2014;Reddeman et al, 2019;Villas et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Mango (Mangiferaindica L.) leaf extracts as ingredient for the formulation of functional beverages with biological activity

Medina‐Saavedra

Herrera‐Corredor

Vargas‐Rivera

et al. 2020

Int J of Food Sci Tech

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The biological activity of mango leaf extracts from different mango varieties was evaluated in terms of total phenolic content, flavonoids, antioxidant activity and inhibition of a-amylase and a-glucosidase enzymes. Leaf extract-based beverages were formulated and evaluated for some physicochemical, microbiological and sensory properties. Results indicated that the extract from 'Tommy Atkins' mango leaves had the highest total phenols content (137.08 mg of GAE g À1 ) and antioxidant activity (DDPH = 38.26 mg TEAC 100 g À1 ; ABTS = 59.13 mg TEAC 100 g À1 ). The beverage formulated with 20% leaf extract presented the highest percentage of antioxidant activity (38.63%) and inhibition of enzymes a-amylase (41.9%) and a-glucosidase (37.53%). All beverages presented a yellow hue and consumers rated it with a degree of liking between 4.8 and 7.3 according to a hedonic scale. Results showed that the biological properties of beverages could be an alternative for the control of free radicals and glucose levels.

show abstract

Preclinical safety evaluation of the aqueous extract from Mangifera indica Linn. (Anacardiaceae): genotoxic, clastogenic and cytotoxic assessment in experimental models of genotoxicity in rats to predict potential human risks

Cited by 12 publications

References 53 publications

Toxicological and genotoxic evaluation of anacardic acid loaded-zein nanoparticles in mice

Toxicological and genotoxic evaluation of anacardic acid loaded-zein nanoparticles in mice

Mangifera indica L.: Assessing the cytogenotoxicity of aqueous extract through micronucleus and comet assays

Mango (Mangiferaindica L.) leaf extracts as ingredient for the formulation of functional beverages with biological activity

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