Preclinical In Vivo Evaluation and Screening of Zinc-Based Degradable Metals for Endovascular Stents

Guillory, Roger J.; Oliver, Alexander A.; Davis, Emma K.; Earley, Elisha J.; Drelich, Jaroslaw; Goldman, Jeremy

doi:10.1007/s11837-019-03371-5

Cited by 22 publications

(38 citation statements)

References 26 publications

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“…There are lots of research works to study the biosafety of different Mg alloys on different cell lines. [52][53][54][55][56][57][58] This study was novel to evaluate the biosafety of AZ31B alloy on nerve cells by applying an assay on the SY5Y cell line. In this study, the effects of Mg 2 + and Al 3+ concentrations on SY5Y cells were obtained by indirect in vitro experiments.…”

Section: Discussionmentioning

confidence: 99%

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Biosafety and biodegradation studies of AZ31B magnesium alloy carotid artery stent in vitro and in vivo

Erişen

Zhang

et al. 2021

J Biomed Mater Res

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Biosafety of AZ31B magnesium (Mg) alloy and the effect of its degradation products on tissues, organs, and whole systems are highly needed to be evaluated before clinical application. This study serves a wide variety of safety evaluations of biodegradable AZ31B alloy on nerve cells. As a result of this in vitro study, the maximum aluminum (Al) ion and Mg ion concentrations in the medium were estimated to be 22 μmol/L and 2.75 mmol/L, respectively, during degradation. In addition, the corresponding cell mortality was observed to be 36% and lower than 5% according to the resistance curves of the cell to Mg and Al ions. Furthermore, the maximum Al ion and Mg ion concentrations in serum and cerebrospinal fluid were detected to be 26.1 μmol/L and 1.2 mmol/L, respectively, for 5 months implantation. Combining the result of in vivo dialysis with the result of ion tolerance assay experiments, the actual death rate of nerve cells is estimated between 4 and 10% in vivo, which is lower than the result of in vitro cytotoxicity evaluation. Moreover, no psychomotor disability during clinical studies is observed. Consequently, stent made of AZ31B alloy with surface treatment is feasible for carotid artery stenosis, and it is safe in terms of cell viability on the nervous system.

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Section: Discussionmentioning

confidence: 99%

“…There are lots of research works to study the biosafety of different Mg alloys on different cell lines 52‐58 . This study was novel to evaluate the biosafety of AZ31B alloy on nerve cells by applying an assay on the SY5Y cell line.…”

Section: Discussionmentioning

confidence: 99%

Biosafety and biodegradation studies of AZ31B magnesium alloy carotid artery stent in vitro and in vivo

Erişen

Zhang

et al. 2021

J Biomed Mater Res

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“…Mo and platinum (Pt) (bioinert control material) wires were cut into 1 cm long segments and sanitized in 70% ethanol before being surgically implanted into live mouse arteries (n = 16 for Mo and n = 12 for Pt implants). Each wire was implanted into the abdominal aorta mouse lumen following adaptation of the surgical protocol reported previously [ 42 ]. Briefly, the wall of the abdominal aorta was penetrated by the sharpened wire, which was then advanced near the arterial endothelium for ∼7 mm before being punctured out of the artery, leaving approximately 5 mm of the metal wire within the arterial lumen.…”

Section: Methodsmentioning

confidence: 99%

In-vivo evaluation of molybdenum as bioabsorbable stent candidate

Sikora-Jasinska

Morath

Kwesiga

et al. 2022

Bioactive Materials

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“…A detailed description of the methods for the morphometric analysis are described in our previous publication. 33 2.6. Statistical Analysis.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Corrosion and in Vivo Response to Zinc Implants with Electropolished and Anodized Surfaces

Guillory

Sikora-Jasinska

Drelich

et al. 2019

ACS Appl. Mater. Interfaces

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Zinc (Zn)-based biodegradable metals have been widely investigated for cardiovascular stent and orthopedic applications. However, the effect of Zn surface features on adverse biological responses has not been well established. Here, we hypothesized that a metallic zinc implant's surface oxide film character may critically influence early neointimal growth and development. Electropolishing of surfaces has become the industry standard for metallic stents, while anodization of surfaces, although not practiced on stents at present, could increase the thickness of the stable oxide film and delay early-stage implant degradation. In this study, pure zinc samples were electropolished (EP) and anodized (AD) to engineer oxide films with distinctive physical and degradation characteristics, as determined by potentiodynamic polarization, electrochemical impedance spectroscopy, and static immersion tests. The samples were then implanted within the aortic lumen of adult Sprague−Dawley rats to determine the influence of surface engineering on biocompatibility responses to Zn implants. It was found that in vitro corrosion produced a porous corrosion layer for the EP samples and a densified layer on the AD samples. The AD material was more resistant to corrosion, while localized corrosion and pitting was seen on the EP surface. Interestingly, the increased variability from localized corrosion due to the surface film character translated directly to the in vivo performance, where 100% of the AD implants but only 44% of the EP implants met the biocompatibility benchmarks. Overall, the results suggest that oxide films on degradable zinc critically affect early neointimal progression and overall success of degradable Zn materials.

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Preclinical In Vivo Evaluation and Screening of Zinc-Based Degradable Metals for Endovascular Stents

Cited by 22 publications

References 26 publications

Biosafety and biodegradation studies of AZ31B magnesium alloy carotid artery stent in vitro and in vivo

Biosafety and biodegradation studies of AZ31B magnesium alloy carotid artery stent in vitro and in vivo

In-vivo evaluation of molybdenum as bioabsorbable stent candidate

In Vitro Corrosion and in Vivo Response to Zinc Implants with Electropolished and Anodized Surfaces

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