Preclinical Feasibility of the Bio-Airbrush for Arthroscopic Cell-Based Cartilage Repair

Dijkstra, Klaas; Huitsing, Roeland P.J.; Custers, Roel J.H.; Kouwenhoven, J. W. M.; Bleys, Ronald L. A. W.; Vonk, Luciënne A.; Saris, Daniël B.F.

doi:10.1089/ten.tec.2019.0050

Cited by 6 publications

(5 citation statements)

References 35 publications

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“…In fact, several cell spraying devices have been introduced in tissue repair and regeneration applications. For example, a bio-airbrush was developed for the treatment of cartilage defects to facilitate cartilage repair [23]. Devices for aerosolizing cell suspensions have been previously used to spray fibroblasts and keratinocytes on leg ulcers to accelerate healing [37,38]; cell-spray auto-grafting technology was performed for deep partial-thickness burns [39]; and the aerosol spraying of bladder urothelial cells and smooth muscle cells was reported to reconstitute muscle segments for bladder augmentation [21].…”

Section: Discussionmentioning

confidence: 99%

“…To our knowledge, the first automated device for cell suspension atomization was a pump-action aerosol nozzle designed to spray cells in vitro [20]. Several other types of spray devices have been subsequently designed, especially for applications involving tissue repair and regeneration [21], including air-brush pistols [22,23], atomizers [24], or spray nozzles [25]. However, these devices proved to have several shortcomings limiting their effectiveness in wound healing.…”

Section: Introductionmentioning

confidence: 99%

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Novel pneumatically assisted atomization device for living cell delivery: application of sprayed mesenchymal stem cells for skin regeneration

Zhang

Yan

et al. 2021

Bio-des. Manuf.

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Large cutaneous wounds pose a severe medical problem and may be deadly in cases when regeneration is impaired. Recently, topical stem cell therapy has been realized as a promising strategy for wound healing and skin regeneration. However, stem cells must be administrated uniformly to the wound area, otherwise treatment will be ineffective, which has been a limitation of current administration methods. Specifically, the delivery pressure and nozzle features of most clinical cell spray devices are unknown, which may significantly affect the viability of sprayed cells and their capacity for proliferation. Herein, we developed a novel pneumatically assisted atomization device (PAAD) in which cell suspensions were uniformly atomized at a low delivery pressure. We optimized the applied fluidic pressure and air pressure to maximize cell survival and function for the application of multiple cell types, while ensuring uniform dispersal across the wound site. Moreover, we found that the application of sprayed umbilical cord-derived mesenchymal stem cells to wound sites significantly accelerated wound healing and promoted appendage regeneration on an excisional cutaneous wound model. Overall, the novel PAAD system delivered living cells uniformly and maintained the viability and differentiation of sprayed cells, strongly suggesting its potential for application in clinical cell therapy, especially for large, irregular, and severe skin wounds.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Novel pneumatically assisted atomization device for living cell delivery: application of sprayed mesenchymal stem cells for skin regeneration

Zhang

Yan

et al. 2021

Bio-des. Manuf.

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“…[147] On the contrary, the in situ strategy has the potential to be paired with arthroscopy techniques. [144,148,149] This is highly advantageous as the vast majority of knee cartilage surgeries are performed using arthroscopic methods. [150] Arthroscopies are shorter and less costly than open surgeries, and lead to faster recovery times with less scarring, and with generally less discomfort and morbidity.…”

Section: Open Knee Arthroscopy and The Potential For Single-stage Pro...mentioning

confidence: 99%

Within or Without You? A Perspective Comparing In Situ and Ex Situ Tissue Engineering Strategies for Articular Cartilage Repair

O’Connell

Duchi

Onofrillo

et al. 2022

Adv Healthcare Materials

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Human articular cartilage has a poor ability to self-repair, meaning small injuries often lead to osteoarthritis, a painful and debilitating condition which is a major contributor to the global burden of disease. Existing clinical strategies generally do not regenerate hyaline type cartilage, motivating research toward tissue engineering solutions. Prospective cartilage tissue engineering therapies can be placed into two broad categories: i) Ex situ strategies, where cartilage tissue constructs are engineered in the lab prior to implantation and ii) in situ strategies, where cells and/or a bioscaffold are delivered to the defect site to stimulate chondral repair directly. While commonalities exist between these two approaches, the core point of distinction-whether chondrogenesis primarily occurs "within" or "without" (outside) the body-can dictate many aspects of the treatment. This difference influences decisions around cell selection, the biomaterials formulation and the surgical implantation procedure, the processes of tissue integration and maturation, as well as, the prospects for regulatory clearance and clinical translation. Here, ex situ and in situ cartilage engineering strategies are compared: Highlighting their respective challenges, opportunities, and prospects on their translational pathways toward long term human cartilage repair.

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“…Meanwhile current work aims at designing a fully closed system which will allow intra-operative cell processing. In addition, first steps in the development of a full-arthroscopic approach with a bio airbrush technique 13 have been taken. In addition, the technology has been expanded to be able to be applied to the hip joint and if indicated could be applied to other joints.…”

Section: Future Prospects Impactmentioning

confidence: 99%

Use of Allogeneic Mesenchymal Signaling Cells (MSCs) to Augment Cartilage Repair

Saris¹,

Windt²,

Custers³

et al. 2022

Operative Techniques in Sports Medicine

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Preclinical Feasibility of the Bio-Airbrush for Arthroscopic Cell-Based Cartilage Repair

Cited by 6 publications

References 35 publications

Novel pneumatically assisted atomization device for living cell delivery: application of sprayed mesenchymal stem cells for skin regeneration

Novel pneumatically assisted atomization device for living cell delivery: application of sprayed mesenchymal stem cells for skin regeneration

Within or Without You? A Perspective Comparing In Situ and Ex Situ Tissue Engineering Strategies for Articular Cartilage Repair

Use of Allogeneic Mesenchymal Signaling Cells (MSCs) to Augment Cartilage Repair

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