Preclinical ex vivo evaluation of the diagnostic performance of a new device for in situ label-free fluorescence spectral analysis of breast masses

Mathieu, Marie‐Christine; Toullec, Alexis; Benoît, Charlotte; Berry, Richard G.; Validire, Pierre; Beaumel, Pauline; Vincent, Y.; Maroun, Pierre; Vielh, Philippe; Alchab, Lama; Farcy, René; Moniz-Koum, H; Fontaine‐Aupart, Marie‐Pierre; Delaloge, Suzette; Balleyguier, Corinne

doi:10.1007/s00330-017-5228-7

Cited by 9 publications

(7 citation statements)

References 23 publications

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“…[29][30][31][32] In addition, an important advantage of FS is the technical possibility of making measurements during minimally invasive procedures using small diameter probes. 25,[33][34][35] We suppose that to solve the problem described, FS can be applied through a minimally invasive approach during primary antegrade decompression of the biliary tract under ultrasound and X-ray control. The additional diagnostic information obtainable by using FS would allow surgeons to assess liver functional reserves before surgery, predict the course of the postoperative period, adjust therapy regimens in a timely manner, including indications for repeat surgery, include extracorporeal detoxification methods, or immediately initiate intensive therapy for progressive liver failure in particularly severe cases.…”

Section: Introductionmentioning

confidence: 99%

Deconvolution of the fluorescence spectra measured through a needle probe to assess the functional state of the liver

et al. 2023

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ObjectivesCurrently, one of the most pressing issues for surgeons in the treatment of obstructive jaundice is the ability to assess the functional state of the liver and to detect and determine the degree of liver failure in a timely manner with simple and objective techniques. In this regard, the use of fluorescence spectroscopy method can be considered as one of the ways to improve the informativity of existing diagnostic algorithms in clinical practice and to introduce new diagnostic tools. Thus, the aim of the work was to study in vivo the functional state of liver parenchyma by the method of fluorescence spectroscopy implemented through a needle probe and assess the contribution of the main tissue fluorophores to reveal new diagnostic criteria.Materials and MethodsWe compared data from 20 patients diagnosed with obstructive jaundice and 11 patients without this syndrome. Measurements were performed using a fluorescence spectroscopy method at excitation wavelengths of 365 and 450 nm. Data were collected using a 1 mm fiber optic needle probe. The analysis was based on the comparison of the results of deconvolution with the combinations of Gaussian curves reflecting the contribution of the pure fluorophores in the liver tissues.ResultsThe results showed a statistically significant increase in the contribution of curves reflecting NAD(P)H fluorescence, bilirubin, and flavins in the group of patients with obstructive jaundice. This and the calculated redox ratio values indicated that the energy metabolism of the hepatocytes may have shifted to glycolysis due to hypoxia. An increase in vitamin A fluorescence was also observed. It may also serve as a marker of liver damage, indicating impaired vitamin A mobilization from the liver due to cholestasis.ConclusionsThe results obtained reflect changes associated with shifts in the content of the main fluorophores characterizing hepatocyte dysfunction caused by accumulation of bilirubin and bile acids and after disturbance of oxygen utilization. The contributions of NAD(P)H, flavins, and bilirubin as well as vitamin A can be used for further studies as promising diagnostic and prognostic markers for the course of liver failure. Further work will include collecting fluorescence spectroscopy data in patients with different clinical effects of obstructive jaundice on postoperative clinical outcome after biliary decompression.

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Section: Introductionmentioning

confidence: 99%

Deconvolution of the fluorescence spectra measured through a needle probe to assess the functional state of the liver

et al. 2023

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“…The functional state of the liver can be estimated by various optical technologies capable of providing new opportunities and diagnostic criteria. Spectroscopy methods are especially promising for researchers, as they allow one to assess the perfusion-metabolic state of tissue in real time and can be used through standard biopsy tools for biopsies of different organs [5][6][7] .…”

Section: Introductionmentioning

confidence: 99%

Optical needle biopsy for multimodal detection of the malignant liver tumours

Zherebtsov

Mamoshin

Shupletsov

et al. 2022

Tissue Optics and Photonics II

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Malignant tumours of the liver are one of the most common causes of cancer death worldwide. The presence and type of tumours can be verified only after histological and cytological analysis of a sample obtained during percutaneous needle biopsy. However, the relatively high probability of false-negative results caused by insufficient tissue samples remains a problem. The introduction of real-time methods to navigate the needle during the biopsy can serve as a solution that reduces the number of mistakes. The paper presents the results of measurements in the murine model of hepatocellular carcinoma by a system equipped with fluorescence lifetime and diffuse reflectance channels, as well as a novel needle optical probe compatible with 17.5G standard biopsy needles. The experimental setup developed allowed us to evaluate the parameters of short and slow fluorescence lifetimes, fluorescence intensities and blood oxygen saturation in tissues. We demonstrated that the set of recorded parameters allows us to distinguish the malignant tissue, control liver tissue and metabolically changed liver tissues near the tumour. We suppose that the proposed technique, supported by machine learning, can significantly decrease the rate of false-negative results obtained with percutaneous needle biopsy.

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“…To discriminate benign and malignant lesions of both superficial and inner organs, a minimally invasive medical device, Probea, with an optical fibre integrated into a 25G needle was developed based on FS. The Probea has been used efficiently for detecting tumor tissues in lung and breast cancer 26 – 28 . A. Keller et al published the research on creating an optical biopsy needle to detect liver tumors by DRS 29 .…”

Section: Introductionmentioning

confidence: 99%

Optical percutaneous needle biopsy of the liver: a pilot animal and clinical study

Dremin

Potapova

Zherebtsov

et al. 2020

Sci Rep

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This paper presents the results of the experiments which were performed using the optical biopsy system specially developed for in vivo tissue classification during the percutaneous needle biopsy (PNB) of the liver. The proposed system includes an optical probe of small diameter acceptable for use in the PNB of the liver. The results of the feasibility studies and actual tests on laboratory mice with inoculated hepatocellular carcinoma and in clinical conditions on patients with liver tumors are presented and discussed. Monte Carlo simulations were carried out to assess the diagnostic volume and to trace the sensing depth. Fluorescence and diffuse reflectance spectroscopy measurements were used to monitor metabolic and morphological changes in tissues. The tissue oxygen saturation was evaluated using a recently developed approach to neural network fitting of diffuse reflectance spectra. The Support Vector Machine Classification was applied to identify intact liver and tumor tissues. Analysis of the obtained results shows the high sensitivity and specificity of the proposed multimodal method. This approach allows to obtain information before the tissue sample is taken, which makes it possible to significantly reduce the number of false-negative biopsies.

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Preclinical ex vivo evaluation of the diagnostic performance of a new device for in situ label-free fluorescence spectral analysis of breast masses

Abstract: • A new device allows in situ label-free fluorescence analysis of ex vivo breast masses • Maximum fluorescence intensity discriminates benign from malignant masses (p < .0001) • Five random measurements allow a high negative predictive value (97.2%).

Cited by 9 publications

References 23 publications

Deconvolution of the fluorescence spectra measured through a needle probe to assess the functional state of the liver

Deconvolution of the fluorescence spectra measured through a needle probe to assess the functional state of the liver

Optical needle biopsy for multimodal detection of the malignant liver tumours

Optical percutaneous needle biopsy of the liver: a pilot animal and clinical study

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